摘要:

Morphological alterations of the glomerulus were induced by infusion of cationized ferritin. After a direct injection of highly cationized ferritin (CF) into the left kidney of rats, endothelial injuries were followed by activation of platelets and the coagulation system after 1‐2h, which occluded capillary loops. In most glomeruli, resolving processes occurred from 2 h onward, leaving a mild thickening of the mesangial region at 7 days. On the other hand, in severely involved glomeruli, capillary loops were still obstructed even at 24 h by hypertrophic and proliferated endothelial cells as well as mesangial cells, instead of platelets and fibrin strands. After this period, exfoliation of podocytes and endothelial cells occurred over a wide area, which resulted in glomerular obsolescence at 7 days. These progressive glomerular injuries were assumed to be closely related to the persistence of CF in glomeruli, which might be caused by disturbances of glomerular clearing systems. Mild proteinuria was only noticed in severely involved cases. It is concluded that an assault on glomerular endothelial cells by cationic macromolecules can cause thrombotic complications leading to glomerular obsolescenc

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01157.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

The influence of sex hormones on induction of intestinal rnetaplasia was examined in 5 week old Crj: CD (SD) rats of both sexes. At the age of 4 weeks, the animals were gonadectornized and given testosterone or dimethyl estradiol (DES). One week after operation, they were irradiated with two 10 Gy doses of X‐rays to the gastric region at a 3 day interval for a total of 20 Gy. At the termination of the experiment, 6 months after the X‐irradiation, the incidence of intestinal metaplasia with alkaline phosphatase (ALP) positive foci in males was significantly higher than in females, in orchidectomized males or orchidectomized plus DES treated rats (P<0.01). On the other hand, the incidence of intestinal metaplasia with ALPpositive foci in normal females appeared lower than in ovariectomized females (P<0.01), and was increased in rats by treatment with testosterone or decreased by DES. Numbers of foci of intestinal metaplasias with Paneth cells and total numbers appeared to increase in males treated with DES. The results suggested a promoting role for testosterone in the development of ALP positive lesions and indicated considerable heterogeneity between intestinal metaplasia subty

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01158.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

An experimental model for induction of gliomas corresponding to human anaplastic astrocytomas and glioblastomas is reported. Eleven week old F344 and ACI rats were given 100 or 200 p.p.m.N‐methyl‐N‐nitrosourea (MNU) solution as their drinking water for 42 weeks. Gliomas were induced at very high incidences (82.5‐92.5%) in each group. Induced gliomas showed apparent evidence of morphologic malignancy by an analysis based on diagnostic criteria of human astrocytomas. All of the gliomas from the killed animals were classified histologically into subtypes according to the classification scheme used in the diagnosis of human gliomas. The majority of macrotumors more than 1 mm in diameter in both strains were diagnosed as anaplastic astrocytomas and glioblastomas. lmmunohistochemically, tumor cells in these tumors were almost negative for glial fibrillary acidic protein, while ultrastructurally neoplastic astrocytes contained glial filaments. A strain difference was observed in the ratio of histological subtypes of macrotumors. In F344 rats, astrocytic tumors diagnosed as anaplastic astrocytomas and glioblastomas of an astrocytic type formed the majority, whereas glioblastomas of mixed oligo‐astrocytic type predominated in ACI rats. The results indicate that MNU‐administration to adult F344 rats may provide a suitable experimental model for gliomas which occur in a

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01159.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

The contrast medium Thorotrast, an agent well known to be carcinogenic, was injected into 400 congeneic Syrian hamsters. The resulting incidence of malignant hepatic tumors such as cholangiocarcinoma, hepatocellular carcinoma and hemangiosarcoma, was significantly higher in the male experimental group than in the control group, and the 50% survival period in the male group was shortened by about 100 days (P<0.01). However administration of the antitumor drug PSK (Polysaccharide Kureha), a protein bound‐polysaccharide extracted from basidiomycete fungi, prevented this carcinogenic effect. The incidence of malignant hepatic tumors in the experimental group was 22.5% compared with 2.8% in the control group (P<0.01) and 10.5% in the PSK‐treated group (P<0.01). PSK also increased the 50% survival period by 61 days (P<0.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01160.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

A cell line derived from medial smooth muscle cells (SMC) was established from the porcine coronary artery by transfection with ori‐defective simian virus 40 plasmid DNA (SV40 DNA). The characteristics of transfected cells (SV40‐SMC) such as cell growth, collagen and non‐collagen syntheses were investigated. SV40‐SMC expressed SV40 large T antigen, c‐myc and c‐myb encoded proteins in the nuclei. SV40‐SMC demonstrated a ‘hills and valleys’ ‐like arrangement in overconfluence and actin filaments upon immunofluorescent staining. Under electron microscopic observation, SV4O‐SMC had larger amounts of synthetic organelles and smaller amounts of filament bundles than those of SMC. SV40‐SMC demonstrated three times higher growth activity and 4.4 times greater cellular density than SMC. Smooth muscle cells did not grow in media containing 5% plasma derived serum (PDS) instead of normal serum, whereas SV40‐SMC proliferated in this medium. SV40‐SMC did not grow in soft agar gel, while HeLa S3 cells, a cell line of human cervical carcinoma, formed colonies in this gel. By immunofluorescent (IF) staining, collagen phenotypes I, Ill, IV and V were detected in both SV4O‐SMC and SMC. However protein synthesis including collagen and non‐collagen was higher in SV40‐SMC than in the control sample. It was concluded that SV40‐SMC were a continuous cell line for vascular SMC regarding morphological characteristics, and demonstrated a higher growth activity, with increased collagen and noncollagen syntheses. This cell line is useful for the investigation of atherogenesis in relation to a proliferation of SMC and an accumulation of extrac

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01161.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

A human hepatocellular carcinoma (HCC) cell line, KIM‐1, was cultured in three different concentrations (0.1, 0.2 and 0.3%) of type‐l collagen gel matrix and its morphologic features, growth kinetics and alpha‐fetoprotein and albumin productions were compared with each other or with those of cells growing on a plastic dish. KIM‐1 cells in any concentration of collagen gel matrix formed various sized threedimensional colonies with compact to trabecular cell arrangement. Larger colonies with a more definitive trabecular cell arrangement, resembling thein vivostructure of HCC, tended to form in the collagen gel matrix of a low concentration. The prolongation of doubling time was identified as the collagen concentration in the gel became higher. The cells on a plastic dish proliferated in a monolayered sheet with a shorter doubling time than others. Ultrastructurally, the cells in collagen gel matrix have more distinct cell membranes, junctional complexes and bile canaliculus‐like structures, and less cytoskeletons than those on plastic dishes, similar to thosein vivo. The productions of alphafetoprotein per 104cells and albumin per 1O5cells were much higher in the collagen gel matrix culture than on a plastic dish in a stationary phase. These data suggest that collagen gel matrix culture is suitable to monitor the morphologic features and protein production of the tumor cells in similar conditions to thosein vivo, and that the three‐dimensional presence of an extracellular matrix is important in cellular proliferation and diff

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01162.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Cellular differentiation of gastric cancer cells allows the classification of cell type into surface mucous cell, pyloric gland cell, intestinal absorptive cell and goblet cell types by mucin histochemistry and pepsinogen (Pg) immunohistochemistry. Surface mucous cell differentiation of gastric cancers of each histologic type has previously been detected by the galactose oxidase‐Schiff (GOS) reaction although this is not always positive in all cases. Mucus granules of surface mucous cells of normal gastric mucosa show an intense reactivity for SH‐9 (monoclonal antibody against CA125‐bearing antigenic molecule fragments). Cathepsin E is also expressed in the cytoplasm of surface mucous cells, weakly in absorptive cells of duodenal villi and occasionally in pyloric gland cells. Expression of SH‐9 reactive mucin and of cathepsin E were therefore investigated as possible additional markers to distinguish between the gastric cancer cell type in 203 primary stomach cancers. SH‐9 reactive mucin was found selectively in GOS positive cancer cells of surface mucous cell type and/or cancer cells unclassified by mocin histochemistry. These latter cells were therefore classified into the surface mucous cell category. Cathepsin E was found mainly in cancer cells of the GOS positive surface mucous cell type and occasionally, in intestinal absorptive and pyloric gland cell types. Galactose oxidase‐Schiff, SH‐9 and cathepsin E reactive or positive cancer cells were found in 145 (71.4%), 151 (74.4%) and 144 (70.9%), respectively, of the 203 primary stomach cancers

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01163.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Sclerosing hemangioma of the lung is a rare benign neoplasm of uncertain histogenesis. In this study, thirteen cases of sclerosing hemangiomas were analyzed by immunohistochemistry for various histochemical markers including surfactant apoprotein. The pale cells were shown to share several lmmunohistochemical markers with epithelial lining cells and were suggested to be immature cells differentiating toward epithelial lining cells. Electron microscopic study failed to characterize the exact nature of pale cells, but it disclosed the precise structure of large lamellar bodies, which are frequently present in the cystic spaces of sclerosing hemangioma. These large lamellar bodies had common morphological characteristics to lamellar inclusion bodies of type II pneumocytes or epithelial lining cells. Eight cases of sclerosing hemangioma obtained freshly at surgery had a quantitative analysis of estrogen and progesterone receptors performed by enzyme immunoassay. The value of estrogen receptors ranged from 0 to 7.8fmol/mg protein. In addition to this quantitative analysis, the immunohistochemical study demonstrated estrogen receptors mainly in the pale cell nuclei of several sclerosing hemangiomas. Estrogen receptors may play an important role in growth, differentiation and surfactant production in the sclerosing hemangloma.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01164.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Clinicopatholigical and cytofluroremetric studies were performed on nine encapsulated (EPC) and 23 non‐encapsulated (non‐EPC) carcinomas of the thyroid gland. The average age of the patients with EPC was 33 years, which was significantly younger than that of those with non‐EPC. The average tumor size of EPC was twice as large as that of intraglandular non‐escapsulated carcinoma. All patients with EPC were alive without disease, but three out of 23 patients with non‐EPC had a recurrence of the disease of died. Cytofluorometric studies showed that the mean nuclear DNA content and percentage of tumor cells in the S‐G2M phase of EPC were lower than that of non‐EPC. According to the DNA histogram pattern, all EPC showed distinct modal DNA values in the diploid or near diploid region of normal cells. However non‐EPC, especially extra‐glandular non‐encapsulated papillary carcinoma, showed a wide variety of DNA histogram patterns. The present study suggested that EPC is a distinct subtype of papillary carcinoma of the throid gland clinicopathologically and the cytofluorometrically it is di

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01165.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

A patient is described with severe IgA nephropathy associated with psoriatic arthritis, idiopathic interstitial pneumonia and brain hemorrhage that developed serially over one and a half years. The histological findings of the renal biopsy showed severe endo‐and extracapillary proliferative glomerulonephritis. Massive IgA deposits were observed by immunoflurorescence not only in the mesangium but also along the capillary walls. Electron microscopy revealed abundant electron‐dense deposits in the mesangial and subendothelial areas. The overlapping or coexistence of these conditions has rarely been repor

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01166.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY