摘要:

Electron microscopic morphometry was undertaken to quantitate the morphological change of the renomedullary interstitial cells (RIC) of hypokalemic rats by using large montages. Two weeks of potassium depletion resulted in an increase of the RIC, which were restricted to the interbundle region of the inner stripe of the outer medulla. The increase of the RIC is characterized by a preferential increase in volume density (+340.0%;P<0.01) and numerical density (+61.4%;P<0.01) in the interbundle region but not in the vascular bundle of the inner stripe or in the inner medulla. The increased RIC in the interbundle region of the inner stripe demonstrated an increase of lipid droplets, which are known to contain prostaglandin precursors. The selective zonal change of RIC with increased lipid droplets is a characteristic lesion of hypokalemic rats and suggests an enhanced vasoactive function of RIC associated with hypokalemic nephropathy.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01115.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

An epidemic outbreak of hepatitis E occurred in an army recruit camp of Yangon, Myanmar, in October 1989. One hundred and eleven patients among 600 residents were hospitalized. As high as 83.7% of these patients were positive for the acute phase antibody against hepatitis E virus by an enzyme‐linked immunosorbent assay developed in our laboratory. Also, 30.6% of 49 symptom‐free residents examined were positive for the antibody.We prepared a stool extract from six patients and inoculated it into 10 rhesus monkeys for a series of three subpassages. All of them developed acute biochemical hepatitis along with an elevation of antibody levels. A rechallenge with viruses of the present outbreak failed to provoke hepatitis in two monkeys that had previously recovered from acute hepatitis caused by an isolate of sporadic hepatitis E of the same area. Similarly, the rechallenge of the sporadic strain did not induce hepatitis in two monkeys that had been previously infected with the epidemic virus. These data suggested that the subjects would obtain neutralizing antibodies against the hepatitis E virus once infected, and many adult inhabitants of the endemic area had no protective antibodies and were still susceptible to hepatitis E infect

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01116.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Immunohistochemical investigations were carried out on the localization and expression of the Ca2+‐dependent intercellular adhesion molecule E‐cadherin in human gingiva and gingival carcinoma. Although E‐cadherin did not appear in the parakeratinized layer of either clinically healthy or inflamed gingiva, it did appear strongly in the prickle layer and somewhat more weakly in the basal layer. Immunogold particles reactive to anti‐E‐cadherin monoclonal antibody in the electron microscopic findings were localized only in the vicinity of the desmosomes of the prickle and basal layers.In the case of gingival carcinoma, although E‐cadherin was strongly expressed in the cells surrounding the keratinized region in the cancer nests, the expression decreased towards the marginal portion of the cancer nests. The distribution of E‐cadherin in these cells may be dependent on the condition of the cancer cells that are potentially invasive.These findings suggest that cells of the parakeratinized layer of gingiva and cells of the marginal portion of the gingival carcinoma nests may easily detach or invade. In addition, the findings suggest that the gingival carcinoma used in this study tended t

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01117.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

The Epstein‐Barr virus‐encoded small nuclear RNA, EBER‐1, has been shown to be a suitable target for thein situhybridization detection of EBV in routinely processed tissue specimens. We evaluated the presence of EBV in thymic carcinoma and invasive thymoma using EBER‐1in situhybridization on formalin‐fixed paraffin‐embedded tissue sections. EBER‐1 expression was demonstrated in a case of lymphoepithelioma‐like thymic carcinoma, but was not detectable in other thymic carcinomas including six squamous cell carcinomas, a clear cell carcinoma and seven invasive thymomas. As reported in three previous cases of EBV‐associated thymic carcinoma, lymphoepithelioma‐like thymic carcinoma was shown to be closely associated wit

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01118.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

We investigated the cell kinetics and morphologies of cholangiocellular carcinoma (CCC) using 48 autopsied or surgically resected cases (47 were adenocarcinoma and the remaining adenosquamous cell carcinoma), all of which were formalin‐fixed and paraffin‐embedded. Cell kinetics were analyzed by counting the number of argyrophilic nucleolar organizer regions (AgNOR) using immunostaining of proliferating cell nuclear antigens (PCNA) and flow cytometric DNA analysis. Dedifferentiation of CCC was positively correlated with AgNOR number (2.22 ± 0.21 in well differentiated, 3.66 ± 0.85 in moderately differentiated and 4.17 ± 0.49 in poorly differentiated adenocarcinomas, respectively). In 22 cases, the labeling index (LI) of PCNA was higher in moderately and poorly differentiated adenocarcinomas (24.0 ± 2.35 and 26.0 ± 4.89, respectively) than in well differentiated ones (10.8 ± 2.14). A majority of well differentiated ones were diploid, while aneuploidy prevailed in moderately to poorly differentiated ones. These data suggest that cell proliferative indices and nuclear DNA analysis of CCC accurately reflect their histological grading. The anatomical location of CCC along the biliary tree had no relation to either of the cell kinetic data. In autopsy cases, the patients with organ and lymph node metastases tended to show a higher DNA index and aneuploidy. This study implies that a combination of several cell kinetic data is valuable for the evaluation of the biological behaviors of CCC, and also supports further studies of cell kinetics of CCC using small‐sized biopsy specimens, as a prognosti

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01119.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

DNA analysis of testicular seminoma (typical seminoma [TS] and anaplastic seminoma [AS]) and spermatocytic seminoma (SS) was performed to evaluate the relationship between the proliferative activity and clinical outcome, and also to determine the proliferative characteristics of SS. Nuclear DNA contents of 15 cases of TS, 17 of AS and three of SS were measured by flow cytometry. Cytofluorometric DNA analysis was performed on three cases of every tumor. All 35 cases showed aneuploidy in the flow cytometry. In comparison with TS, AS tended to have a higher percentage of G2M phase in one cell cycle, having more cell cycle numbers and containing cells of various DNA values. Many mitotic figures with larger atypical cells that characterize AS could be explained by these results. In SS, the diploid formation by the small cells and the appearance of large cells with a maximum DNA value of 40C were demonstrated. These ploidy characteristics were considered to result in the morphological expression of large, small and intermediate cells. The application of both flow cytometry and cytofluorometry to the same cases was beneficial.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01120.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

A case of extracranial meningioma in the parapharyngeal space is reported. A 24 year old woman presented with swelling and tenderness of the parapharyngeal region. A tumor was palpable in this region, and the tumor was surgically removed. Macroscopically the tumor occurred from the portion between the axis and atlas. Histologically the tumor cells, which had oval nuclei and a slightly eosinophilic cytoplasm, proliferated in fibrous connective tissues to form small nests. As the cell borders were not clear, the tumor structure appeared to be syncytium‐like. Immunohisto‐chemically the tumor cells were positive for anti‐vimentin antibodies, anti‐S‐100 protein antibodies and anti‐epithelial membrane antigen (EMA) antibodies in part. Electron microscopically the tumor cells had complex interdigitations of their adjacent plasma membranes. These were studded with many desmosomes. Bundles of intermediate filaments were visible in the cytoplasm. On the basis of the clinical, histological, immunohistochemical and electron microscopical features, the tumor was diagnosed as extracranial meningotheliomatous meningioma. The parapharyngeal space is an extremely rare location for extracranial meningioma, and our case is the first in Japan as far

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01121.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Three cases of pulmonary hypertension caused by tumor emboli to the lungs are described. Two of the three cases had a clinical diagnosis of pulmonary thromboembolism until surgical embolectomy, and the other had a diagnosis of primary pulmonary hypertension. Autopsy disclosed chondrosarcoma, choriocarcinoma and gastric cancer as the primary tumors, respectively. Pulmonary vascular obstruction due to tumor embolism leading to pulmonary hypertension is a previously rare clinical entity, and obstructed pulmonary vessels are believed to tend to be small vessels. We compared the autopsy and radiological findings and concluded that pulmonary tumor embolism involved not only the small peripheral arteries but also the segmental and/or lobar arteries.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01122.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

A 38 year old man died suddenly of widespread lymphoid neoplasm after a short clinical course. Histologically the tumor was composed of pleomorphic large cells that often demonstrated phagocytic activity. Moreover, histochemical and immunohistochemical tests revealed that the tumor cells had the phenotypes of both anaplastic large cell lymphoma and histiocytic cells. lmmunogenotyping failed to demonstrate any lymphoid nature. This case might be categorized as anaplastic large cell lymphoma with histiocytic phenotypes.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01123.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb01124.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY