摘要:

Mallory bodies (MBs) were isolated from the livers of 8 alcoholic patients and a malignant hepatoma occurring in a non‐alcoholic patient. MBs were isolated in large quantity and high purity. The isolates were devoid of liver cell organelles except in the case of malignant hepatoma where dense and membranous material resembling nuclear substances and endoplasmic reticulum were found. The "nuclear" material had continuity with MBs. Electron microscopic examination suggested the presence of a dense body and very fine filaments within in situ and isolated MBs in addition to the tubular structure of so‐called MB fibers. Amino acid analysis indicated that the major component of MBs is a protein which does not contain unusual amino acids or any particular amino acid in a large quantity to characterize this protein. The isolated fractions were solubilized in 6 M guanidine hydrochloride by sonication or in 1% sodium dodecyl sulfate (SDS) with 2 mercapto‐ethanol and 8 M urea. The solubilized MB protein produced 3 peaks by Sephadex G 100 chromatography and at least five intense protein bands by SDS polyacryl‐amide gel electrophoresis. Serum sample and the fractions from control livers contained several protein bands which were similar to the major components of isolated MB protein. These morphological and biochemical findings suggest that MBs are not homogeneous protein, but they are probably complexes of various proteins and that some components of MBs are present in normal hepa

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1976.tb00522.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Changes in the regional lymph nodes from guinea pigs stimulated with oxazolone painting or LPS injection were examined by light and electron microscopy at various intervals. The proportions of T and B cells in the nodes were estimated by rosette formation technique30,34and weights of the thymus and spleen were measured at the same time. The first event occurring in the nodes after antigenic stimulation was increase in number and activity of post‐capillary venules (PGV) which resulted in augmentation of an influx of small lymphocytes from the recirculating pool. Lymphocytes which streamed into the nodes promptly flowed out through the sinus endothelium into the pool. The second event was specific accumulation of committed lymphocytes35. The proportion of T cell in the node suspension reached a peak 4 days after oxazolone painting when morphological changes became maximum and the thymus enlarged. On the other hand, in LPS group, B cells reached a peak on day 5 or 6 when relatively immature germinal centers increased in the nodes and decreased on day 7 because of their differentiation into plasma cell

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1976.tb00523.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

In an attempt to clarify the kinetics of Increase of mast cells, autoradiographic studies were performed on the mice which received a painting of 20‐methylcholanthrene on the skin. In the first experiment, mice received 20‐methylcholanthrene painting on their back for two, four and eight weeks. A fourty‐eight hours’cumulative labeling with 3H‐thymidine was performed directly before sacrifice. No labeled mast cells were found in the painted skin, despite a marked increase of mast cells in number. In the second experiment, mice received 20‐methylcholanthrene painting for thirty‐one days. A twenty‐four hours’cumulative labeling was performed three, five, seven, fourteen, twenty‐one and thirty‐one days before sacrifice. A high labeling index of mast cells, up to 61.29%, was obtained in each group. When the time lapse between the time of cumulative labeling and the sacrifice is long, the labeling index is higher In the subcutaneous tissue, and when the time lapse is short, the labeling index is higher in the subepidermal tissue. From these results it was concluded that; 1) An increase of mast cells is due predominantly to the proliferation of precursor cells and their transformation into mast cells. 2) The proliferation of precursor cells occurs mainly in the subepidermal layer of the skin. 3) After the transformation, mast cells may migrate into the dee

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1976.tb00524.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

The ultrastructure of eight 7,12‐dlmethylbenz (a) anthracene (DMBA) or 4‐nltroquinollne‐N‐oxide (4‐NQO) Induced malignant tumors of placental and uterine origin in the rat were studied. Three of eight tumors had the ultra‐structural characteristics of choriocarcinoma. The other five tumors had different fine structures. Two were classified as squamous cell carcinoma, one was as a leiomyosarcoma and the other two were undifferentia

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1976.tb00525.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

Sarcoma botryoides (embryonal rhabdomyosarcoma) originating from the common bile duct is reported in a 4‐year‐old girl. Grossly, the tumor is characterized by multiple polypoid and grape‐like projections into the lumen of the common bile duct and shows plate‐like thickening of the common bile duct wall. On multiple cut‐sections these polypoid masses are soft and light yellow white except for multifocal hemorrhage. Histologically the tumor consists of undifferentiated mesenchymal cells with a myxomatous appearance; rhabdomyoblasts with varing degree of maturation are scattered here and there within the matrix. These cells are characterized by a strap and racket shape with abundant eosinophilic cytoplasm and elongated cytoplasm with cross striations. The histogenesis of the tumor may be related possibly to an abnormal differentiation of the primitive mesenchymal cells toward striated mus

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1976.tb00526.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

A case of a malignant epithelial tumor of tail of the pancreas in a 3‐year‐old Japanese girl is presented. This is the fourth case reported with ultrastructural study. This type of pancreatic carcinoma of the infant has no endocrine granules or endocrine function. The ultrastructural study suggests that the tumor is derived from a primitive epithelial cell with differentiation toward acinar c

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1976.tb00527.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

The female patient was diagnosed as having Von Gierke's disease at 14 years of age, based on clinical manifestations, laboratory examination and liver biopsy. At 19 years of age she had uremia and died from its deterioration at 24 years of age. The parents were consanguineous, and a 27‐year‐old sister is presently hospitalized for renal Insufficiency with hepatomegaly. On autopsy, the patient's kidneys were highly contracted and contained a number of small cysts, mainly in the medulla. Histological examination indicated periglomerular fibrosis, glomerular hyalinization, tubular atrophy or cystic dilatation and intersitial fibrosis with round cell infiltration. These findings correspond to Fanconi's familial juvenile nephronophthlsis, except for age. The liver was markedly enlarged and Indicated severe glycogen deposits, but the kidney did not contain glycogen deposits. It can, therefore, be presumed that the renal lesions were not a secondary consequence of long‐term glycogen deposits but that renal and hepatic lesions were associated with each

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1976.tb00528.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

An autopsy case of a 9 years and 5 months old gargoyle girl diagnosed as Sanfilippo B syndrome by the biochemical demonstration of a large amount of heparan sulfate in urine and some organs and of deficiency of α‐N‐acetyl‐D‐glucosaminidase in the liver and brain was reported. The morphological changes characterized by cytoplasmic swelling and vacuolization were more generalized than those which had been described in previously reported cases. Histochemically, accumulation of variable amounts of acidic glycosaminoglycans and compound lipids, presumably gangliosides and phospholipids, was substantiated in the vacuolated cells of various visceral organs and in the ballooned neuronal cells. Ultrastructurally, numerous inclusions found in these cells were largely divided Into two types; flocculent reticulogranular and osmiophilic, mostly laminated materials, many of which were bound by a single unit membrane. Enzyme cytochemistry proved acid phosphatase activity in the majority of the inclusions in fibroblasts and nbrocytes biopsied from the skin. Rough endoplasmic reticulum in these cells was markedly dilated with reticulogranular materials. The morphological changes of the present case and their pathogenesis were d

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1976.tb00529.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY

摘要:

An autopsy case of Behçet disease which was considered to be typical both clinically and histopathologically was reported. Cerebral abscess was also noted. Consequently it was presumed that some of the findings which had been called softening, demyelination, cavitation among others as the lesions of Neuro‐Behçet might be closely related to abscess. Besides suppurative endoaortitls which was thought to be of interest as the lesions of Vascular‐Behçet was observed. In order to substantiate the above, a general review of the literature of Behçet disease w

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1976.tb00530.x

出版商:Blackwell Publishing Ltd    

年代:1976

数据来源: WILEY