ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb01486.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Cells and histocompatibility antigen systems involved in graft‐versus‐host reactions (GVHR) were analyzed using Simonsen's splenomegaly assay employing various combinations of donor and F1hybrid recipients mice. Most of the cells proliferating in spleens of mice undergoing GVHR were J11d+, and had histological features of cells of the hematopoietic lineage. The proportions of CD3+T cells were decreased in the spleens. Disparity at minor histocompatibility determinants of AKR, I‐E and H‐2D regions between B10.A(4R) donors and (4R × AKR) F1recipients evoked only negligible GVHR. On the contrary, disparity at H‐2K and/or I‐A regions appeared to be sufficient to permit induction of full GVHR. When surface markers of donor spleen cells were analyzed, it was shown that Thy‐1+and/or MEL‐14+cells caused a strong effect on GVHR. Further, either CD4+or CD8+T cell subset could induce significant GVHR. However, synergistic influences of these two T cell subsets on one another in GVHR were observed. The present results raise the possibility of using Simonsen's assay along with a number of reagents to identify the contribution of subsets of T lymphocytes and in analyzing precise contributions of cellular components from both donor and recipient, and also of the target antigen systems of the recipient that contribute to early events

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb01487.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

To investigate the effects of volume loading of the heart, the endocardium was studied histologically and ultrastructurally. Thirteen adult female beagle dogs were used. An arterio venous shunt was constructed between the right common carotid artery and the right external jugular vein in nine animals to induce a volume load. Four animals were used as controls. All were kept for 6–12 months. Heart weight, relative heart weight (heart weight/body weight), cardiac output index, stroke volume index and volumes of both ventricles in the experimental animals were significantly larger than in the controls. Shunted blood volume was significantly correlated with heart weight. The endocardium of the left ventricle in the experimental animals showed elastofibrosis and was significantly thicker than in the controls. In 5 hearts, it was more than 20 μm thick and its endothelial cells showed many long microvilli with a very thick basement membrane (1.5–2.0 μm). The thickness of the endocardium was significantly correlated with the heart weight, relative heart weight and cardiac output index, within 1% 5% and 5% risk, respectively. These endocardial changes were thought to be induced by hemodynamic changes in the left ventricle of the volume‐loaded heart, probably being correlated with changes in cardiac function and mor

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb01488.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The effects of TSH on peroxidase activity (PO) and thyroglobulin (TG) production were investigated using primary cultures of thyroid cells obtained from patients with Basedow's disease (Basedow's cells). PO activity of cultured cells and TG concentration in the culture medium were measured by biochemical and sandwich enzyme immunoassays, respectively. The addition of TSH (10 mU/ml/day) to the medium did not increase the cell number but did increase the PO activity and TG concentration. It took more than 3 days for the PO activity of cells cultured with TSH (stimulated group) to reach a level twice that of cells cultured without TSH (control group), whereas 2 days of incubation with TSH was sufficient for increasing the TG concentration. When actinomycin D (AD) was added to the medium on the first day of 3‐day incubation with TSH, the stimulatory effect of TSH on PO was completely blocked and the TG concentration was reduced to half that of the control group. AD given to the stimulated group on the last day of induction produced no inhibitory effect on the induction of PO activity by TSH, but reduced the TG concentration to almost half that in the stimulated group. An electron microscopic study of Basedow's cells cultured with AD and TSH failed to reveal any cytopathic change. The findings of the present study suggested that in cultured Basedow's cells TSH induces PO activity and TG production through the synthesis of new messenger RN

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb01489.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Rheumatoid arthritis (RA) is one of the immune complex (IC) diseases in which lymphoid germinal centers (GCs) are found in the synovial tissue. Simultaneously, patients with RA often show swelling of lymph nodes. The morphology and function of the lymph node GCs in patients with RA is not clear. The aim of this study was to evaluate the differences in morphology and immunoreactions to complement (C) components, their receptors, and lgM‐rheumatoid factor (RF) between synovial GCs and lymph‐node GCs in RA. Furthermore, the relationship between these immuno‐reactive substances and follicular dendritic cells (FDCs) in GCs was investigated. The tissues examined were 41 RA synovial specimens, seven RA lymph nodes with massive lymphadenopathy, and 10 non‐RA lymph nodes. The number of synovial GCs was relatively decreased in comparison with lymph‐node GCs in RA, and the diameter of each synovial GC was smaller than that of each lymph‐node GC. The synovial GCs were edematous and less cellular, and moreover, those from RF‐seronegative cases were smaller than those from RF seropositive cases. On the other hand, the lymph‐node GCs in RA were larger, more cellular and hyperplastic, but contained more tingible‐body macro‐phages (TBMs) and neutrophils. In the GCs of both synovial tissues and lymph nodes in RA, early C components (C1q, C4, C3c, C3d), IgM RF, and C3b receptor (C3bR) and C3d receptor (C3dR) were expressed as a lacy network by light microscopy, and were demonstrated on the surfaces of FDCs and lymphocytes, and in the intercellular spaces by electron microscopy. Furthermore, immuno‐staining for dendritic reticulum cells (DRC, DAKO DRC1) was observed in a lacy pattern by light microscopy and on the cell surface of FDCs by electron microscopy. In the GCs of non‐RA lymph nodes, early C components, C3bR, C3dR, and DRC showed a similar reaction pattern, but IgMRF did not. Consequently, no marked difference in immunoreactions in the GCs, except for the immunoreactions of late C components, was found between synovial tissues and lymph nodes in RA. On the basis of these findings, we discuss the possibility of

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb01490.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The pathologic features of a case of spinal pencil‐shaped softening (PS) were studied by detailed step‐sectioning and reconstruction using a three‐dimensional (3D) model. The spinal cord was obtained at autopsy from a patient who had developed paraplegia due to extradural involvement of lung cancer. Above and below the area of transverse necrosis were PSs containing necrotic debris. They compressed the surrounding tissue and extended longitudinally over several segments. The PS was continuous with the area of transverse necrosis. These findings support the hypothesis that PS is formed by penetration of necrotic debris. The 3D model revealed that the PS was cylindrical to spindle‐shaped. It showed a remarkable change in width along its course, with an unusual expanding shape. The PS was mainly located in the ventral part of the posterior column but was also observed in other locations in the segment near the transverse necrosis. Detailed stepsectioning showed that the PS changed in width mainly in response to intrapial pressure, and that it occurred where the parenchymal tissue was fragile. The expansion produced by penetration of necrotic debris may thus play an important role in the morphogenesis of this unusual lesion. Furthermore, the condition of the surrounding tissue may also be an important factor influencing the width and locatio

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb01491.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

We report the immunohistochemical and ultrastructural features of a case of duodenal carcinoid (somatostatinoma) combined with cutaneous type von Recklinghausen's disease in a 65‐year‐old woman. The duodenal tumor located at the 2nd portion was composed of tumor cells arranged in a trabecular, glandular or nest‐like pattern, occasionally associated with eosinophilic globules in the glandular structures. The tumor cells mostly showed strong immuno‐reactivity with anti somatostatin antibody. Electron microscopy revealed that every tumor cell contained intracytoplasmic granules with electron‐dense cores, 100–300 nm in size, in varying numbers, and intracytoplasmic lumina were rarely detected. The present case including identical cases reported so far suggest that the disease entity is likely to be a combination of duodenal somatostatinoma and von Recklinghausen's disease and/or phaeochromocytoma. This is the tenth case of duodenal carcinoid associated with von Recklinghausen's disease and/or phaeochromocytoma in the world literature and the third case reported as duodenal soma

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb01492.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Combined choriocarcinoma and adenocarcinoma in the lung of a 71‐year‐old Japanese male is reported. In the upper lobe of the right lung (S1+ 2), a choriocarcinoma coexisted with an adenocarcinoma, but distinct metastatic lesions were noticed separately in the lungs, kidney, bone marrow and lymph nodes. Although immunohistochemical examination revealed intensely positive reactivity with anti‐human chorionic gonadotropin (HCG) in trophoblastic cells, weak immunoreactivities were also observed in a few cells with anti‐human placental lactogen (HPL), anti‐pregnancy specific β glycoprotein (SPI), anti‐epithelial membrane antigen (EMA), anti‐carcinoembryonic antigen (CEA), anti cytokeratin (keratin) and KM‐93 (lung adenocarcinoma‐associated antibody). In the adenocarcinoma, the tumor cells were positively stained for CEA, EMA, keratin and KM‐93, but there were no positive reactivities for HCG, HPL and SPI. These findings suggest that primary choriocarcinoma of the lung may arise through dedifferentiati

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb01493.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb01485.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY