摘要:

Spindle‐cell sarcomas in the somatic soft tissue and soft parts, including fibrosarcoma, leiomyosarcoma, malignant fibrous histiocytoma (MFH), and malignant schwannoma were examined by electron microscopy in order to delineate the most reliable cellular features for their diagnosis. Fibrosarcoma consisted largely of fibroblastic cells and leiomyosarcoma cells were packed in forming small cell groups with constant junctional complexes of nexus and zonula adherens types. MFH showed variable cellular features containing the cells with myofibroblastic and histiocytic differentiation. Malignant schwannoma was characterized by tumor cells having slender cytoplasmic processes with overlapping or interdigitation and thick basement membrane. These ultrastructural features were contributory to the differential diagnosis of the sarcomas examine

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb00350.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Morphologic and radiologic studies were undertaken on 26 human embryos and fetuses to determine the stage and site of the earliest bone marrow formation. Up to the 10th week of gestation, primary bone marrow is not present anywhere although the intramembranous ossification occurs in the maxilla and mandible and also in the middle portion of the clavicle. At the 11th week of gestation, X‐ray examination showed in two fetuses the bone formation in the clavicle, scapula, maxilla, mandible, and the diaphysis of the long bones. Serial sections of these fetuses revealed that the primary bone marrow occurs first in the middle portion of the clavicle. From a series of our embryological studies, the concept of the mononuclear phagocyte system which involves the bone‐marrow‐derived monocytic origin of tissue macrophages, is not accepted, at least, on the origin of Kupffer cells in human fetal l

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb00351.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The fine structure of the hepatic sinusoids of 81 human embryos and fetuses and their development from 5 to 12 weeks gestation were studied. At 5 weeks gestation, sinusoid‐like structures and Kupffer‐like cells were observed between liver cell cords. Between 6 and 8 weeks gestation the sinuosids were completely developed. Definite Kupffer cells appear at this developmental stage, when the bone marrow has not yet formed. Floating macrophages form cell aggregates in the sinusoids which contact endothelial cells and settle as Kupffer cells. Erythroblastophagia is observed in Kupffer cells and macrophages. The endothelial linings are closed, with the attenuated cell processes and intercellular junctions between the adjoining endothelial cells. No transition was observed between Kupffer cells and endothelial cells. The findings suggest that Kupffer cells in the human embryo are extrahepatic in origin and that they reach the sinusoids via the circulatory system. Ito cells, which store fat, originate from mesenchymal cells in the septum transver

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb00352.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

A total of 84 autopsied and 10 operative pancreatic carcinoma cases were studied to Investigate their morphological behavior, by light and electron microscopies. The autopsied materials were classified into 70 duct cell carcinomas, 11 undifferentiated carcinomas including 2 giant cell carcinomas simulating giant cell tumor of bone, and 3 endocrine cell carcinomas which might be called carcinoid or oat cell carcinoma. No acinar cell carcinoma was found. Duct cell carcinomas were further subclassined into 37 large and 24 small duct‐forming adenocarcinomas, 8 adenosquamous carcinomas, and 1 cystadenocarcinoma. The majority of undifferentiated carcinomas were considered to be of ductal or ductular cell origin. Rare tumors, such as cystadenocarcinoma, giant cell carcinoma, and endocrine cell carcinoma, were shown as case presentations. Electron microscopic features of duct cell carcinoma and its related findings were also presented. These studies should be pursued, because it may be imporant clinically and disclose, in the future, possible differences in etiology or effective therapeutic agents among the categories, although the classification, made from a viewpoint of histogenesis, did not reflect prognostic differences at the presen

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb00353.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The incidence and the behavior of endocrine cells In carcinomatous tissues were studied in 84 autopsy cases and 10 surgical or autopsy cases of pancreatic carcinoma, by light and electron microscope, respectively. It was found that the islets persistently survived in the carcinomatous tissues. In large duct type carcinomas, the islets showed unique familiarity with the carcinomatous ducts, which may be interpreted as suggesting interactions between ductal cells and islet cells in pancreatic carcinogenesis. Aside from these survivor islet cells, carcinomas exhibited different degree and kind of differentiation to endocrine cells, depending upon their histological types. Namely, neoplastic argyrophil cells were found at the incidence of 82% (large duct type carcinoma), 39% (small duct), 43% (adenosquamous), and 18% (undifferentiated one). Moreover, argyrophil cells with their apexes stretching far to the neoplastic lumen were found only in large duct type carcinoma and cystadenocarcinoma. In three cases of carcinoid or oat cell type carcinoma, argyrophil cells were diffusely scattered and irregular in shape. These findings further justify the classification, reported in the previous article. The differentiation to endocrine cells was confirmed also by electron microscope.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb00354.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Aminopeptidase (AMP) activity of leukemic cells in acute non‐lymphocytic leukemia (36 cases) was examined by comparing peroxidase (PO) activity. The examination of AMP activity was useful for the diagnosis of non‐lymphocytic leukemia in 19 of 36 cases rather than PO activity. Especially in acute monocytic leukemia its tendency was conspicuous. Furthermore, in 5 cases AMP was positive whereas there was no evidence of PO activity. On the contrary, PO was evident in 11 cases. The cases which revealed the same degree of both activities were only 4 and in 2 cases they were completely negative. Although the total number of PO negative cases was 7, ultrastructural endogenous myeloperoxidase reaction was clearly observed in granules of leukemic cells in all cases examined (6 cases). The diagnosis and classification of leukemia must be made using several techniques, and especially in non‐lymphocytic leukemia cases, not only PO activity but also the examination of AMP activity is important. Therefore, one must be prudent in applying the FAB classification in acute leu

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb00355.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Whole brain cells, taken from Sprague‐Dawley rats of various ages: 13‐, 14‐, and 16‐day fetuses, and 2‐ and 13‐day‐old animals were transformedin vitroby adenovirus type 12 (Ad. 12). Immature or dedifferentiated astrocytes (As cells), neurons, and ependymal cells could clearly be identified by conventional staining methods and scanning electron microscopy. Ependy‐moglioblast‐like cells, oligodendrocytes, and undifferentiated cells were also recognized. Sequential observation disclosed that a probable candidate for the target of transformation by Ad. 12 might be As cell which was the most predominant cell type. The transformation rate was highest in the secondary cultures from 16‐day fetuses and lowest in the secondary cultures from

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb00356.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Using neonates born from experimental autoimmune myasthenic rabbits, the authors demonstrated antibodies to acetylcholine receptor (AChR) in the newborn sera. By radioimmunoassay, antibody titers of 1‐day‐old neonates were roughly one seventh to one ninth of the mothers. At 8 weeks postpartum, the antibody was no longer detectable. Ultrastructural observations of the intercostal muscles of the neonates revealed two types of changes. The first type was degenerative alterations in the postsynaptic membrane. The second type of change, which was morphometrically analyzed, was immaturity of postsynaptic membrane structure with underdeveloped secondary synaptic clefts. After 28 days postpartum, these changes were not visible, thereby indicating that the process is reversible as the antibody titer decreases. These results suggested the possibility that the antibody to AChR, transferred transplacental, arrested the development of postsynaptic structure, although reversible, by blocking of the receptor sites in the end‐

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb00357.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Immunoperoxidase staining for myoglobin was performed on formalin‐fixed, paraffin‐embedded tissues of normal human skeletal muscle and rhabdomyosarcomas in order to assess its potential diagnostic value. Myoglobin‐positive cells were observed in normal adult and fetal muscles, and in 13 of 26 rhabdomyosarcomas. In normal skeletal muscle, the intensity of staining for myoglobin varied in individual cells. Of the 13 myoglobin‐positive rhabdomyosarcomas tested, 6 showed cross striations in tumor cells, whereas the others did not. Myoglobin‐positive tumor cells were not demonstrated in two rhabdomyosarcomas in which cross striations were observed. In other types of tumors examined that sometimes resembled rhabdomyosarcomas, no myoglobin was detected. Thus tests for myoglobin by the immunoperoxidase method seem helpful in identifying rhabdomyosarcomas and confirming their histological

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb00358.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

An electron microscopic immunohistochemical localization of thyroglobulin (TG) using PAP methods from Epok‐812 embedded tissues has been made in human thyroids obtained from 15 patients with treated Basedow's disease aged 13 to 43 years. Follicular cells vary considerably in shape and content of cytoplasmic organelles depending mainly upon the size of follicles. Reaction product for TG coincided well with previous investigations studied by electron microscopic autoradiography and is strongly positive in most follicular lumina having no relation to their size, reabsorbed colloid droplets or small subapical vesicles. The reabsorbed colloid droplets are rarely observed in cells surrounding distended follicles and more frequently appeared in small follicles. The reabsorbed colloid droplets after fusion with lysosomes show a variable amount of reaction products from intense deposition to a complete absence of product indicating hydrolysis of TG by lysosomal enzymes. There is no reaction product in rough endoplasmic reticulum or Golgi apparatus in spite of positive reaction product in the subapical vesicles. These subapical vesicles are composed of both endocytotic and exocytotic vesicle

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1983.tb00359.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY