摘要:

To deliniate the histopathological features of liver diseases seen in Japanese alcoholics, 130 Japanese alcoholic patients were studied in comparison with 238 American alcoholic patients. In Japan female alcoholic patients were extremely rare. The male to female ratio was 127 to 3 in Japan and 152 to 89 in U.S.A. Although all aspects of alcoholic liver disease did exist in Japan, typical cases of acute alcoholic hepatitis with alcoholic hyalins were remarkably smaller in number (9.2% in Japan versus 39.5% in the U.S.A.). The severity based on histopathological findings was also less in the Japanese cases. In contrast to this, 46 percent of Japanese alcoholic patients had chronic hepatitis, and the incidence of multilobular cirrhosis was much greater in Japan wherewith higher prevalence of viral hepatitides.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1980.tb01346.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Detection of high incidence of antinuclear antibodies (ANA) was reported in young homozygous rhino mice employing formalinized chicken erythrocyte nuclei as substrate for indirect immunofluorescence (IF) assay. The titers of ANA heightened with increasing age, and attained to 1:1024 by the time mice reached 5 months of age. The occurrence of ANA was associated with development of splenic and hepatic fibrosis, glomerulonephritis and abnormalities of lymphoreticular tissue. The granular deposits of IgG and C3 detected by direct IF were initially found at the basement membrane of dermal‐epidermal junction of rhino mice aged 2.5 months. These deposits distributed progressively in the fibrotic areas of spleen and liver, and renal glomerular tufts at 5 months of age. Dense deposits revealed by electron microscopy were found in the regions where IF of IgG and G3 was observed. Acid buffer eluates from liver and kidney contained IgG reactive with nuclear antigens. Importance of homozygous rhino gene was discussed in relation to development of autoimmune disorders of these mic

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1980.tb01347.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The process of granule formation in mast cells was investigated with young mast cells in the milky spot of adult rats, seven days after an intraperitoneal injection of distilled water. Serial ultrathin sections were made and examined with an electron microscope to reveal the following results. The early step of granule formation is observed as a complex which consists of an irregularly shaped narrow vestibular space and several primary aggregate granules which are bowl‐like in form, attached to the vestibular space and contain a few subgranules. The vestibular space has microvilli. The precursor materials seem to be secreted from the microvilli and transported through the vestibular space into the primary aggregate granules, being coagulated to become subgranules. As the subgranules increase in number, the primary aggregate granules become aggregate granules which grow further to their maximum size. With further addition of the materials, the aggregate granules become, through a step of ropy cord granule, mature dense homogeneous granules. The complex seems to remain as a unit after the maturation of granules and to react as a whole to any stimulu

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1980.tb01348.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Three cultured cell lines of human lung cancer with varied degrees of thromboplastic and fibrinolytic activities were injected subcutaneously or intravenously into congenitally athymice nude mice, and their fate was observed with light and electron microscopes. All the cell lines formed solid tumor masses at the sites of subcutaneous injection. When the cancer cells with high or moderate thromboplastic activity were injected intravenously, marked platelet aggregation and fibrin deposition, followed by neutrophilic infiltration, were seen around the cancer cells arrested in pulmonary arterioles or capillaries. Platelet aggregation and fibrin deposition were less conspicuous, when the cancer cells with low thromboplastic activity were injected. No metastatic foci were found 2 weeks after the intravenous injection of each cancer cell line. These results suggest that thromboplastic activity of human cancer cells is closely related to thrombus formation at the sites of lodgement and that thrombus develops without participation of immunological mechanisms.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1980.tb01349.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Several experimental methods inducing murine amyloidosis were tested using six strains of mice including congential asplenic mice and athymic nude mice. Traditional induction method of amyloidosis by casein injections failed to cause amyloidosis in C3H/He mice. Single injection with complete Freund's adjuvant reinforced with Mycobacterium butyricum was also unable to induce amyloidosis in C3H/He, CBA, and BALB/c mice. Six or four injections with complete Freund's adjuvant at an interval of once a week successfully induced amyloidosis in CBA mice with high incidence but not in C3H/He, ICR/SLC, and BALB/c mice. Athymic nude mice with genetic background of BALB/c and congential asplenic mice, cross‐bred with G57BL/6 × C3H, were free from amyloidosis after six or fourteen injections with the adjuvant. Experimental amyloidosis in mice, therefore, might mainly depend on the strain of mice used and the induction method chos

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1980.tb01350.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

Leupeptins, protease inhibitors, suppress the appearance of experimental amyloidosis in CBA mice induced by the injections with complete Freund's adjuvant. This substance, however, should be administered continuously from 1 week prior to amyloid induction to the end of the experiment. On the other hand, Trypan blue, inhibitors of lysosomal enzymes, accelerates experimental amyloidosis in the mouse model above‐mentioned. Trypan blue is effective when given either prior to or at the same time of the initiation of amyloid induction. Organic Germanium has not been confirmed to be a potent suppressor of experimental murine amyloidosis but the experimental group administered this substance continuously from 1 week prior to the induction shows a rather low incidence of amyloidosis, and the average number of amyloidotic organs per affected mouse is about half of that of the control group. The suppression and acceleration of experimental murine amyloidosis presented here are a useful tool for investigating the pathogenesis of amyloidosi

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1980.tb01351.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

An autopsy case of erythremia with sideroblastic tumor cell proliferation is described. A 60‐year‐old man was admitted to the hospital due to general fatigue and anorexia. Bone marrow aspiration revealed abnormalities in erythropoiesis (megaloblasts, 4%; sideroblasts, 84%; ring‐formed, 39%, and PAS‐positive, 5%). Therapy was directed to pulmonary tuberculosis. Anemia was not improved despite repeated whole blood and platelet transfusions. Serum iron and percentage saturation of the total iron‐binding capacity rose during the course. Administration of vitamin B12, B6or folic acid was ineffective. INAH was replaced by its derivative, IHMS, during the course, but the population of sideroblasts especially of ring‐sideroblasts was invariably large (78%‐100% and 39%‐65% for total sideroblasts and ring‐sideroblasts, respectively). He died with increasing abdominal pain and jaundice after three months’hospitalization. Main autopsy findings were: diffuse proliferation of atypical erythroblasts in the bone marrow, systemic lymph nodes, liver, spleen and kidneys. Most of the cells positively stained with iron. Tuberculosis of lungs with cavity formation. Discussion is focussed on the relationship between erythremia and s

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1980.tb01352.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

The patient had been employed in an asbestos factory for four years from the age of 16. Five years ago, she complained of sputum and cough, and she was treated for chronic bronchitis. From March, 1977, When she was 53 years old, hydrothorax and ascites increased, her weight decreased and she was hospitalized for cachexia. The clinical diagnosis of malignant diffuse mesothelioma was made on the presence of atypical cells in the effusions. Atypical cells showed a positive colloidal iron staining test and positive hyaluronidase digestion test. Asbestos bodies were found in the sputum. The patient died on February 1978. Postmortem confirmed asbestosis and mesothelioma which was scattered over the pleura, pericardial sac, diaphragma, peritoneum and pancreas. In addition, bronchiolo‐alveolar cell type lung cancer was found localized in the lower lobe of the left lung. The electron beam diffraction disclosed the asbestos as amosite (brown asbestos

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1980.tb01353.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

In an autopsy case of sporadic amyotrophic lateral sclerosis, there were intracytoplasmic eosinophilic inclusions of the Bunina type in motor neurons. Electron microscopically, these bodies were observed as amorphous substances, irregularly deposited around the endoplasmic reticulum to form masses. Similar small masses were also visible in mitochondria. These substances did not stain for acid phosphatase. By analytical electron microscopy they contained silicon. Bunina bodies, therefore, seem to be due to deposition of some metabolite, but their nature is still obscure.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1980.tb01354.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY

摘要:

A case of a 44‐year‐old man with hepatic form of glycogenosis was presented. The patient had abdominal distension and muscular weakness. The glucose tolerance test showed a diabetic pattern, though he had hypoglycemia in fasting state. The fructose tolerance test showed an ability of conversion from fructose to glucose. The double glucagon test showed no rise of blood glucose in fasting state but a rise 2 hours after meal. These symptoms and laboratory data supported the clinical diagnosis of type III glycogenosis. At autopsy, glycogen was markedly deposited in the liver, and slightly in the kidneys and heart. The glycogen pooled in the hepatic cells histochemically showed a normal reaction to several glycogen stainings. Electron microscopy by using Thiéry's method revealed that the pooled glycogen particles were clearly arranged as rosettes measuring 1.000Å in largest diameter composed of clustered monoparticulates. There were marked hyalinization of the islets of Langerhans containing amyloid. As to its pathogenesis, this change can be interpreted as a morphological expression of the hypofunction of β‐cells ascribed to long‐standing hy

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1980.tb01355.x

出版商:Blackwell Publishing Ltd    

年代:1980

数据来源: WILEY