摘要:

Radiofrequency catheter ablation of the atrioventricular (AV) node or bundle of His was performed in 12 adult mongrel dogs. The aim was to create chronic incomplete AV block (first‐ and second‐degree AV block) and to examine the histopathology of the ablated lesions. However, the late electrophysiological results (2 4 weeks follow up) were various: normal in 2 dogs, mild PR prolongation (<50%) in 2 dogs, first‐degree AV block (PR prolongation a 50%) in 2 dogs, second degree AV block in 2 dogs, complete AV block in 4 dogs. The maximally ablated area (%) of the atrioventricular conduction system in serial histologic sections from dogs with these conditions was 69%, 75%, 89.5%, 95% and 99.5%, respectively. The number of intact conduction cells at the maximally ablated site varied from 6 to 30 in the four cases of incomplete AV block. The mean ablated volume (%) of either the AV node or penetrating His bundle correlated roughly with the degree of AV block. The ablated lesions were well demarcated and almost replaced by dense fibrous tissue at 4 weeks. Interruption (3 dogs) or thinning (1 dog) of the endocardial elastic lamellae was detected, in association with endocardial thickening (mean 913 μm). Endocardial thrombi were found in 3 dogs (2 fresh, 1 organized). We conclude that radiofrequency catheter ablation does not cause severe complicated lesions. Several possible conditions for creating chronic incomplete AV block are discussed. Acta Pathol Jpn 41: 487–4

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1991.tb02513.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Bone marrow stromal cells play an essential role in the proliferation and differentiation of hematopoietic stem cells (1,2). As a means of analyzing of the bone marrow microenvironment immunohistochemically, we attempted to produce a rat monoclonal antibody against the murine preadipocyte line HI derived from long‐term bone marrow culture (LTBMC) of C57BL/6 mice (3,4). A newly established monoclonal antibody, designated R4‐A9, was obtained from a hybridoma prepared by fusion of Y.B2/ 3.0Ag20(YO) rat myeloma cells with spleen cells of LEW rats immunized with HI cells. The immunofluorescence of live HI cells showed that the antigen reacting with this antibody was strongly expressed on the cell surface. The specificity of R4‐A9 was assessed immunohistochemically on frozen sections of various tissues from normal adult mice. R4‐A9 demonstrated specificity for hematopoietic stroma in bone marrow and spleen. No staining was observed in thymus, lymph nodes or other tissues examined, with the exception of Leydig cells in the testis and the endothelium of small arteries in several organs. Detailed immunohistochemical observations at both the light microscopy and electron microscopy level showed that R4‐A9 selectively reacted with the sinusoidal endothelium, peri‐sinusoidal adventitial cells (5) (adventitial reticular cells (6)) and intersinusoidal reticular cells (5) and the reticular cells of the splenic red pulp. These findings indicate that reticular cells and the endothelium of the bone marrow possess the common cell surface molecules recognized by R4 A9. SDS‐PAGE analysis showed that R4‐A9‐immuno‐precipitated proteins had a molecular mass of 100 kDa under reducing conditions. These data show that R4‐A9 is highly specific for reticular cells and the endothelium of murine bone marrow and spleen, providing a new approach for detailed structural analysis of the constituents of bone marrow stroma. Acta Pathol

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1991.tb02514.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

A permanent cell line established from a xenograft of neuroblastoma which occurred in a 5 year old girl was investigated for its morphological and biological characteristics. The cultured cells were tumorigenic in nude mice. Microscopically, each tumor consisted of small round to polygonal cells with irregular nuclei and prominent nucleoli, corresponding to the features of the primary and xenografted tumor cells. Electron microscopic examination revealed that both the transplanted tumor cells and the cultured cells contained scanty microtubules and dense‐core neurosecretory granules. Chromosome analysis of this cell line showed monosomy for chromosomes 1,10,19 and X, and structural rearrangements involving chromosomes 8, 17 and 20, in addition to numerous double minutes. The N‐myconcogene was found to be amplified 40 to 80 fold in the transplanted and cultured tumor cells, as well as in the primary tumor cells.In situhybridization with a digoxigenin labeled uridine‐triphosphate N‐mycRNA probe detected abundant mRNA in the tumor cells. This neuroblastoma line may become a valuable in vitro experimental model system for studies aimed at better characterization of neuroblastoma. Acta Pathol Jpn 41: 507 51

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1991.tb02515.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

In order to assess the expression of different cytokeratins in the collecting duct cells (CDCs) of the human kidney, three consecutive sections were stained with periodic acid‐Schiff, CAM 5.2, and AE‐1 (CAM 5.2 recognizes cytokeratins #19,18,8 and AE 1 #19,16,15,14,10 of Moll's catalog.), respectively. By comparing these sections, it was found that most CDCs in the inner medulla were both CAM 5.2 and AE 1 positive, whereas in the outer medulla and cortex, 77% of the CDCs were both CAM 5.2 and AE 1 positive, 15% CAM 5.2 positive and AE 1 negative, 8% both CAM 5.2 and AE‐1‐negative, and 0.4% CAM 5.2 negative and AE 1 positive. Recent studies have shown that most CDCs express low‐molecular weight cytokeratins #7,8,18 and 19(17,18,19,20). Of these cytokeratins, CAM 5.2 recognizes cytokeratins #8,18,19 and AE‐1 recognizes cytokeratin #19. Therefore, most CDCs belong to one of the following three major types; 1. Those positive for cytokeratins #8,18 and 19 (CAM 5.2 and AE 1 positive), 2. Those positive for cytokeratins #8 and 18 and negative for #19 (CAM 5.2‐positive and AE 1 ‐negative) and 3. Those negative for cytokeratins #8,18 and 19 (CAM 5.2 and AE 1 negative). A few CAM 5.2 negative and AE 1 positive cells were thought to express high molecular weight cytokeratins. The significance of these various cytokeratin expressions is discussed. Acta Pathol Jpn 41

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1991.tb02516.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Pathologic changes in superficial temporal arteries (STA) and middle meningeal arteries (MMA) biopsied from 15 patients with moyamoya disease (MD) who had undergone cerebro temporal arterio synangiosis were studied histologically, ultrastructurally and immunohistochemically. The main pathologic features were: proliferation of smooth muscle cells (SMCs) and thickening of the intima, degeneration and destruction of SMCs in the media and intima, and the presence of condensed organelles in necrosed SMCs or the interstitium among SMCs, or both outside and within the elastica interna (El). The El had become thin, porous, fragmented and was even absent in some segments. These changes are different from those of other forms of angiopathy, but identical with those at the ends of internal carotid arteries (ICA) reported by us previously, being pathognomonic for MD. These changes in the STA and MMA reveal that MD involves not only the ICA but also the intra and extracranial branches of external carotid arteries. The medial necrosis of SMCs seems to be the primary injury of the arterial wall in MD. STA tissue blocks from two cases of MD were stained immunohistochemically. By electron microscopy, IgG, IgM, and C3 positive granules were observed on the ER of endothelial and intimal cells. Further studies on more cases are needed to determine whether an immunoreaction has occur ed in these arteries. Acta Pathol Jpn 41: 521 530, 1991.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1991.tb02517.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

We have carried out cytochemical and ultrastructural examination of human leukemic cells showing basophil/mast cell features derived from patients with acute myelogenous leukemia or basophilic crisis in chronic myelogenous leukemia. Leukemic cells in each case initially showed meta‐chromasia with toluidine blue and various degrees of positivity for astra blue. Other cytochemical results showed considerable variety among cases. The number of granules increased in short‐term culture in every case. Ultrastructurally, small membrane‐bound granules with or without myelinoid bodies or glycogen particles were present in immature blasts, followed by production of other granule types. In some cases, leukemic cells before and after liquid culture contained the typical basophil granules with or without myelinoid bodies, but the matrix was more loose than normal. Granules showing whorl or scroll matrix profiles, which were typical for mast cells, were present in two cases. In one case, immatue leukemic cells contained theta granules, and some mature forms after short term culture contained typical basophil/mast cell granules as well as theta granules. Leukemic cells occasionally contained multivesicular granules predominantly. These results indicate that leukemic cells with basophil/ mast cell features show a heterogeneous configuration and contain abnormal granules differing from normal ones. This abnormal granulopoiesis may be attributable to the results of leukemic events and may be a hallmark for recognition of leukemic basophils/mast cells. Acta Pathol Jpn 41: 531–53

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1991.tb02518.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

The authors examined the immunohistochemical and ultra‐structural characteristics of mixed small cell/large cell carcinoma (mixed subtype) of the lung which has been newly categorized as anaplastic small cell lung carcinoma. The 34 cases of small cell lung carcinoma examined consisted of 18 cases of pure small cell carcinoma (pure subtype), 12 cases of the mixed subtype and 4 cases of combined small cell carcinoma. Immunohistochemically, the number of immunoreactive tumor cells for chromo‐granin A was smaller (P<0.05) and that for creatine kinase BB was relatively larger in the mixed subtype. Sialyl Les i antigen (SLX), one of the SSEA 1 related carbohydrate antigens, was expressed specifically in the mixed subtype. Ultrastructurally, tumor cells in the mixed subtype had smaller amounts of neurosecretory granules and larger amounts of desmosomes than those in the pure subtype (P<0.05, P<0.005, respectively). These results suggest that the mixed subtype may show more epithelial than neuroendocrine differentiation and that SLX may be a specific marker for the mixed subtype to be used in pretherapeutic diagnosis. Acta Pathol Jpn 41: 540 551, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1991.tb02519.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

A 7 year old boy with giant cell fibroblastoma (GCF) of the skin and subcutaneous tissue of the right chest wall is described. To date, the histogenesis of GCF has not been clarified. The reason for the diversity of immunohistochemical data among various authors may be because the specimens studied were from only part of the lesion, or reduction of antigenicity through the preparation process. However, our findings based on studies of many specimens from various parts of the tumor for accurate immunohistochemical evaluation suggest that GCF may be a myofibro histiocytic tumor. Recently, the suggestion that GCF is a juvenile form of dermatofibrosarcoma protuberans (DFSP) has been reported. In addition to the present case, we performed immunohistochemical examination of 10cases of definitely diagnosed DFSP for comparison. The immunohistochemical characteristics of these two neoplasms were concordant. However, from clinical and morphological viewpoints, it seems premature to recognize GCF as a juvenile form of DFSP. Acta Pathol Jpn 41: 552–560, 199

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1991.tb02520.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

A case of fatal intestinal mucormycosis in a 57 year‐old female hemodialysis patient who had received desferrioxamine (DFO) for aluminum overload is reported. The focus of fungal infection was not found until the intestine had been resected surgically. DFO is being used with increased frequency in dialysis patients to treat aluminum or iron overload. Recently a possible link between DFO therapy and mucormycosis has been suggested. In the management of hemodialysis patients, the potential risk of mucormycosis with DFO therapy should be considered. Acta Pathol Jpn 41: 561–566, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1991.tb02521.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

A case of necrotizing fasciitis caused by beta hemolytic streptococci is reported. A 66‐year‐old man was admitted because of pain and swelling in the right buttock. Rapid application of aspiration cytology made it possible to diagnose necrotizing fasciitis with bacterial infection. Unfortunately, however, the patient died of cardiac arrest due to hyperpotassemia 11 h after admission. Mortality from this disease is most often related to failure in recognizing it early. Rapid diagnosis and early treatment is mandatory in order to save the patients’life. We emphasize the usefulness of rapid aspiration cytology, despite the unfortunate outcome in the present case. Acta Pathol Jpn 41: 567 572,

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1991.tb02522.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY