摘要:

Twenty‐nine cases of invasive lobular carcinoma were analyzed, based on three aspects of the histology: 1) cellular features such as a monotonous proliferation of uniform small cells, 2) a single file or targetoid arrangement, and 3) loss of cell cohesion or dissociation of tumor cells. Twenty‐four tumors which fulfilled these three criteria were appraised as cases of conventional lobular carcinoma, in a classic sense, while five others were a variant of this tumor. Individual tumor cells of lobular carcinoma were estimated to be well differentiated, both morphologically and functionally, revealing well developed intracytoplasmic organelles and a high percentage of α‐lactalbumin content in the cytoplasm. Nevertheless, the tumor itself was characterized by a lack of any particular structural differentiation in the arrangement of cells. Based on the observation of the histologic features, invasive lobular carcinoma was subclassified into three groups, in situ predominant, intermediate, and diffuse infiltrating and with a definite correlation to the age of the patient and to the prognosis. Validity of this classification indicates that lobular carcinoma progresses gradually, even in the invasive phase, and can be categorized as a slowly growing subset of mammary car

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01470.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Eighty gastric and colorectal lesions including cases of dysplasia and carcinoma were studied by immunohistochemical techniques to investigate the behaviour of laminin, type IV collagen and fibronectin. Their distribution was examined on frozen and formalin‐fixed, paraffin‐embedded samples. In the same lesions, interruption, fragmentation and absence of basement membrane (BM) antigen‐staining were observed. Carcinomas with well differentiated glandular structures were always surrounded by a well defined BM as in normal, non‐pathological tissues. On the contrary, undifferentiated areas arranged in nests or sheets were usually negative for laminin and type IV collagen. Anomalous BM staining was strictly related to the degree of differentiation of tumor tissue, while no correlation existed between carcinoma staging and BM antigen presence, either in gastric or colorectal neoplasms. Immunostaining with antibody against type IV collagenase showed a massive positivity in the early gastric carcinomas examined, while in colorectal cancer only granulocytes sh

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01471.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

In order to achieve a clear distinction between Hodgkin's disease (HD) and peripheral T cell lymphomas, a microphotometric analysis was made on 5 μm thick sections, by illustrating a nuclear density scattergram (NDS) and a superimposed DN? histogram (S‐DN? H) which was composed of cell populations by nuclear size versus DN? content. HD cells (including mononuclear cells, Reed‐Sternberg's cells, and lacunar cells) showed the oblique zonal cluster (OZC) on the NDS, indicating their proliferative ability. In particular, the small HD cells in lymphocytic depletion (LD) meant their high proliferation. The lymphocytes among HD cells showed an euploid diploid pattern. The HD cells of the borderline cases, having lymphocytes with twisted or distorted nuclei, proliferated with higher and wider ploidy than the HD cells in LD. The population of HD cells in the borderline cases was much larger than that of the HD cell‐like cells in adult T cell lymphoma (ATL), pleomorphic type. The distorted lymphocytes fall in the euploid diploid range and had smaller nuclei than the medium‐sized neoplastic T cell

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01472.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The present study is a chronological morphological examination on the effects of collagen gel matrix on regeneration of severed sciatic nerves. The nerves (5 mm length) were resected, and both the distal and proximal stumps were inserted into a silicone tube with 5 mm gap in between. In the test side, the gap in the tube was then injected with liquid collagen which gells in the tissue when reconstructed with a certain buffer solution. The gap space in the tube of the control side was left empty. In a chronological examination of the tissue in the tube, considerably more rapid growth of sprouting axons toward the distal stump in the test side was revealed in comparison with the control side. The cells, including both fibroblasts and larger Schwann cells, were less in number. More orderly directions were observed in the collagen matrix than in the control tube. The result indicates that regeneration of the peripheral nerves in the silicone tube can be improved, by using appropriate exogenous fine materials, collagen matrix.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01473.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Twelve biopsied thymuses taken from 4 cases with myasthenia gravis (MG group) and 8 cases without myasthenia gravis (control group) including 2 thymoma cases in each group were immunopathologically investgated in relation to myoglobin (Mb). Mb positive cells of various degrees were detected in all thymuses of both groups, and immunoelectron microscopical examination disclosed that Mb positive cells corresponded to interdigitating reticulum cells and myoid cells in non‐neoplastic thymuses, and neoplastic epithelial reticular cells in thymomas. Anti‐Mb antibody staining by direct immunoperoxidase technique revealed positive localization to the lymphoid cells in the thymuses of 2 cases of MG group with thymoma. In addition, indirect immunofluorescent study with the serum of each case which was applied to the normal human skeletal muscle, showed positive staining of the sarcoplasm in 3 cases of MG group, including 2 thymoma cases, and using peroxidase labeled serum IgG F(ab')2of the same patients this anti‐muscle antibodies were proved to be against both postsynaptic cytosol and sarcoplasm of the extraocular muscle of the guinea pig. From these results, it was suggested that Mb may conduct itself as a homologous antigen between the thymus and the skeletal muscle in the myasthenic patient with thymoma, and in the thymus the interdigitating reticulum cell, the myoid cell, or the neoplastic epithelial reticular cell may retain or produce Mb as an antigen‐presenti

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01474.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The effects of magnesium (Mg) deficiency on the coronary arteries of 27 Yorkshire swine were studied by light and electron microscopy. The experimental animals were divided into 4 groups which received the following supplements: Group I, basal ration with adequate Mg (540 mg/kg diet), Group II, basal ration with insufficient Mg (270 mg/kg diet) Group III, 10% milk powder with adequate Mg (540 mg/kg diet), Group IV, 10% milk powder with insufficient Mg (270 mg/kg diet). Serum analysis indicated that dietary low Mg supplementation decreased cholesterol levels and increased phospholipid concentrations significantly. The highest magnitude and incidence of intimal thickening were observed in the coronary arteries of Group IV (p<0.003). No significant intimal thickening was detected in any of the other groups. Ultra‐structural studies revealed a greater frequency of degenerated cells in Group III and IV (p<0.01). Numerous calcifications were observed in only Group IV. These data suggest that moderate Mg deficiency can promote atherosclerosis in combination with some atherogenic diet, and that the presence of smooth muscle cell degeneration is important in order for a magnesium deficiency to exert an effect on the coronary artery of swin

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01475.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

We reported five cases of glomerulonephritis developed in the course of rheumatoid arthritis (RA), which had no apparent relation with therapeutic agents. Systemic angiitis was observed in patient 1 and overlapping of systemic lupus erythematosus (SLE) with RA was implied in patient 2, while the other three patients did not show these changes. Renal biopsies were performed in all of the cases and glomerular deposition of immune complexes was suggested by both the immunofluorescent and electron microscopic findings, though the amount was variable. Furthermore, cases of glomerulonephritis in patients with RA were reviewed in the literature and classified into three groups: (1) occurring in association with angiitis, (2) overlapping with SLE or other collagen diseases, and (3) developing without the above two factors. Group 3 was further divided into two subgroups according to immunologic abnormalities. All cases included in the three groups can be regarded as glomerulonephritis of RA. The cases of group 2, however, can more preferentially be considered to be caused by SLE or some other collagen disease that has overlapped with RA. Deposition of immune complexes was suggested in most of the cases examined with immunofluorescence. Various changes were seen in the glomerulonephritis of RA presented here (patients 1, 3, 4, and 5) and reviewed in the literature (groups 1 and 3). Immune complexes might be involved in the pathogenesis of glomerulitis.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01476.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The alterations of actin filaments in the glomerular epithelial cells of adriamycin‐induced nephrotic rats were studied by electron microscopy and immunohistochemistry using both of the peroxidase‐antiperoxidase and avidin‐biotin methods. After the administration of adriamycin, the glomerular epithelial cells showed cell swelling, deformation of cell shape, vacuole formations, retraction or fusion of foot processes, detachment of the plasma membrane from the glomerular besement membrane (GBM), and an increase of cellular organelles. In parallel with these alterations, abnormal distribution of intracytoplasmic microfilaments of 5–6 nm in diameter was observed, which formed clusters, particularly gathering densely along the plasma membrane of the fused foot processes that abutted the GBM. Immnohistochemically, actin was demonstrated on the site of the above described filaments. The above results suggest that actin filaments may closely relate to the morphological maintenance of the glomerular epithelial cells and to their function

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01477.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Confronting cisternae of the endoplasmic reticulum recognized in tumor cells of 7 cases of osteosarcoma were presented. They were found in the mitotic cells as well as in the cytoplasms of interphase cells. The more the mitotic cells were observed in 1 μm‐thick sections, the more frequently those membranous structures were encountered in the corresponding ultrathin sections. In the interphase cells, such structures were located around Golgi apparatus or close to the nucleus. Occasionally, they were composed of a pair of closely apposed cisternae of the nuclear membrane and the rough endoplasmic reticulum. These results seem to indicate that the nuclear envelope which is disrupted and reformed during mitosis in rapidly proliferating cells takes part in the formation of the confronting cisternae of the endoplasmic reticul

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01478.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

This paper reports an autopsy case of a 5‐year and 11‐month‐old girl with generalized subcutaneous lipomatosis, megalencephaly, and macrodactyly. Marked emaciation and poor prognosis were the characteristic findings of this case. We thought that this case was identical with a rare syndrome which was initially reported by Bannayanin 1971. Autopsy disclosed diffuse lipomatosis in the thoracic and abdominal cavity, and infiltration of fat tissue to the pancreas. Hyperplasia of the small intestinal mucosa and Peyer's patch, and a pedunculated polyp of the sigmoid colon were the unique findings which could not be seen in the previously reported

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01479.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY