摘要:

The proliferative activity of Ito cells in acute liver injury induced by carbon tetrachloride (CCl4) was studied by light and electron microscopic autoradio‐graphy. At 48 hours after a single intraperitoneal injection of CCl4, the livers of the mice given vitamin A per os for preceding 9 days and those of the mice without vitamin A‐pretreatment were removed. Small tissue blocks of each group were respectively incubated at 37°CC for 1 hour in culture medium containing3H‐thymidine. After CC14, injection, perisinusoidal and sinusoidal cells adjacent to centrilobular necrotic liver cells increased in number and size. Some of them were labelled by3H‐thymidine. On the other hand, the perisinusoidal and sinusoidal cells in the peripheral zone in which liver cells are not markedly degenerated nor necrotic showed no noticeable increase in number. They contained very few or no silver grains after3H‐thymidine. In control mice the labelling of perisinusoidal cells was hardly observed. Electron microscopic autoradiography revealed that most of the labelled perisinusoidal cells in the centrilobular zone possess characteristics of Ito cells in their location and in the fine structures such as the presence of small fat droplets, well‐developed rough endoplasmic reticulum, and Golgi complex in the cytoplasm. These findings indicate that Ito cells incorporate3H‐thymidine in DNA synthesis after hepatocellular necrosis resulting in cell proliferation. ACTA PATHOL. JPN. 35: 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1985.tb01429.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Three granulomas in the portal tract and 9 bile ducts with typical features of chronic non‐suppurative destructive cholangitis (CNSDC) associated with dispersed epithelioid.cells were examined by electron microscopy in 10 patients with primary biliary cirrhosis (PBC). Vesicular epithelioid cells, which contained numerous single‐membrane bound vesicles, predominated in the granulomas. On the other hand, immature epithelioid cells and activated macrophages were more often observed near epithelial cells of the bile ducts with CNSDC than in the granulomas. These macrophages seemed to be activated by epithelial cells of the bile ducts and develop into epithelioid cells. Honeycomb‐like membranous labyrinths containing electron dense substances were frequently observed in epithelioid cells and were likely a special form of phagosome. The substances in the labyrinths seemed to be derived from organellae of necrotic cells and extracellular interstitial tissues. Subplas‐malemmal linear densities (SPLD) were observed at the cytoplasmic boundary and at intracytoplasmic membranous labyrinth. The roles of SPLD were discussed. ACTA PATHOL. JPN. 35 : 1309–1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1985.tb01430.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

The histological changes during the acute phase of infection in the livers of marmosets inoculated with hepatitis A virus were examined. The acute phase was divided into four stages according to the liver enzyme changes and ser‐ological markers for the viral infection (Stage I, II, III, IV). Round cell infiltration in the portal tracts was first recognized in Stage I. Localization of parenchymal changes was predominantly periportal in Stage I, II, and IV, whereas the lesion was diffuse in Stage III. Hepatitis A virus antigen (HAVA) was widely distributed but spotty and the largest amount of HAVA was found in Stage II by immunofluorescent and immunoperoxidase study. By electron microscopy the endoplasmic reticulum was altered in the liver cells and in some area there was interaction between the hepatocytes and lymphocytes. These findings suggest that hepatocellular damages seen in this model are the result of immune response rather than cytotoxic effect. ACTA PATHOL. JPN. 35: 1319–1331, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1985.tb01431.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

An electron‐microscopic study was carried out using chimpanzees’livers infected with experimental hepatitis B for the elucidation of intracellular development of HBV‐associated ultrastructures and extracellular release of HBV. Core particles were first detected in the nucleus of liver cells at around the time of the first seropositiveness for HBsAg, and then in the cytoplasm. Subsequently, their budding into endoplasmic reticular cisterna was seen together with other core particles in the surrounding cytoplasm. Dane‐like particles were seen in the cisterna, and also extracellularly nearby a liver cell with a marked proliferation of microvilli at the onset of liver cell injury. Thereafter, core‐like particles were seen within electrondense amorphous material at the site of the contact between liver cell and lymphocyte. The above sequence of features suggested us the assembly of core particles and surface envelope at the cisternal membrane of endoplasmic reticulum, and a reversed pinocytosis whereby Dane or HBV particles were released extracellularly. The filamentous structures within endoplasmic reticular cisternae, which were thought to be HBsAg, were never detected. ACTA PATHOL. JPN. 35: 1333

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1985.tb01432.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

An ultrastructural study on the hepatic bile secretory apparatus and bile ductules was carried out using liver biopsy specimens of six chimpanzees with experimental hepatitis B. Although biochemical cholestasis was very mild or lacking and light‐microscopic evidence of cholestasis was not evident, various ultrastructural changes which had been described in association with cholestasis were detected. The significance of these ultrastructural changes in relevance to cholestasis was difflcult to determine, nevertheless electron‐microscopic examination could possibly be the most sensitive means for the diagnosis of cholestasis. These ultrastructural changes appeared to be indifferent to the necrotizing process of liver cells in experimental hepatitis B. ACTA PATHOL. JPN. 35 : 1343–1358,

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1985.tb01433.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Ultrastructural studies on liver cell necrosis and the interaction of lymphocyte and liver cell were carried out in experimental hepatitis B in chimpanzees. Two types of liver cell necrosis were identified. One was a lytic necrosis, and the other was a coagulation necrosis. Both types of liver cell necrosis were closely associated with the apposition of lymphocytes. The interaction (or close contact) of lymphocyte and non‐necrotic liver cell infected with HBV was also detected. There were two distinct patterns of the contact. One was the direct contact, and the other was the contact with the intervention of electron‐dense fuzzy material containing 20 to 22 mu spherical particles and 51 to 55 mu double‐layered spherical particles. The ultrastructural characteristics of lymphocytes in each pattern of the contact were different. The results suggested that the pathogenesis of liver cell necrosis in hepatitis B was closely associated with the action of lymphocytes, and two modes of lymphocytic reaction were conceivable. ACTA PATHOL. JPN. 35 : 1359 ‐137

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1985.tb01434.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Monoclonal antibody (MoAb) to rat liver plasma membranes was prepared by hybridization of mouse immune lymphocytes with mouse myeloma cells, and was identified by the immunodiffusion method in a fraction of IgM secreted from the hybridoma thus obtained. In indirect immunofluorescence tests, specific fluorescence was detected only on the surface of rat hepatocytes, but neither on the cells from other organs of the rat nor on the hepatocytes of other species of animals, suggesting that the antibody may be organ‐ and species‐specific. When the primary culture rat hepatocytes, labelled with isotopic cromium (51Cr), were treated with the MoAb together with complement, a specific release of51Cr from the cells was found shortly after treatment, accompanied with bubbling of the cell membranes, and a significant release of51Cr was observed at an MoAb concentration of 15μg/ml or more. Without complement, or with inactivated complement, these reactions were not observed. These facts suggest strongly that the cell surface of the hepatocytes was damaged by the MoAb in the presence of complement. ACTA PATHOL. JPN. 35: 1375–1383

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1985.tb01435.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Sequential changes in the development of ethylnitrosourea (ENU)‐induced rat brain tumors were examined histologically, immunohistochemically, electron microscopically and autoradiographically. In 47 Sprague‐Dawley rats transplacentally administrated with ENU, 95 brain tumors developed, including 76 microtumors less than lmm in diameter. Microtumors were found mainly in the paraventricular area, but some were found in the peripheral brain tissue. They were composed of small tumor cells which had round dark nuclei and scanty cytoplasm immunohistochemically negative for Leu 7 and glial Abrillary acidic protein (CFAP). The 19 macrotumors were mature gliomas, 3 of which histologically corresponded to oligodendrogliomas and 16 to mixed gliomas. The tumor cells of the former had small round nuclei with distinct perinuclear halos and a small amount of cytoplasm positive for Leu 7. The latter were chiefly composed of polygonal cells having large round nuclei and rich cytoplasm positive for CFAP. An autoradiographic study using3H‐thymidine revealed that the labeling index of the tumor cells was high in mixed gliomas and microtumors, but low in oligodendrogliomas. It may be concluded that the constituent cells of microtumors correspond to glioblasts or migrating neuroglias, which gradually mature to form oligodendrogliomas or astrocytomas. ACTA PATHOL. JPN. 35 : 1385–139

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1985.tb01436.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

In order to clarify the relationship between cellular characteristics of rat brain tumors and the administration time of chemical carcinogens, the incidence and morphology of microtumors in the offspring of five pregnant Sprague‐Dawley rats injected with ethylnitrosourea (ENU) intraperitoneally on the 11th day of gestation (Group I) were compared with those of six pregnant rats injected with ENU on the 18th day of gestation (Group II). Twenty‐four microtumors and 6 macrotumors were obtained in Group I and 58 microtumors and 5 macrotumors in Group II. Histologically, the macrotumors of Group I showed a poorly differentiated pattern which corresponded to primitive spongioblastomas in comparison with those of Group II which resembled human mature oligodendrogliomas, astrocytomas or mixed gliomas. The constituent cells of the microtumors of both groups were immature and were morphologically similar to each other. Immunohisto‐chemically, the cells of microtumors of both groups were negative for Leu 7 and glial fibrillary acidic protein. Microtumors in Group I showed more infiltrative proliferation than those in Group II and had indistinct borders. The3H‐thymidine labeling index of the constituent cells of Group I was significantly higher than that of Group II (p<0.01). This study revealed that the constituent cells of microtumors of Group I, in spite of being morphologically similar with those of Group II, possess a greater growth potency than the latter. This difference in growth potency is responsible for the difference in the histological maturity of the macrotumors in each group. The difference in morphological maturation of enlarged gliomas induced by ENU appears to depend on the imperceptible difference of the target cell maturation at the initiation of oncogenesis. ACTA PATHOL. JPN. 35 : 1397–1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1985.tb01437.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY

摘要:

Thirty‐four cases of carcinoma of the human large intestine were studied employing a battery of histochemical techniques to identify and characterize mucosubstances, including galactose oxidase‐Schiff (GOS) reaction and stain‐ings with horseradish peroxidase‐labeled lectins. The results disclosed that the goblet cell‐type much (GCM) of the left colon differed from that of the right colon in containing 8‐O‐acetylated N‐acetylneuraminic acid (NANA) more abundantly and 8‐O‐acetylated NANA‐(αFuc)‐βGal type terminal structures. The right colonic GCM, on the other hand, was shown to contain α Fuc‐βGal type predominantly. Surface coat‐type mucin (SCM) showed consistent histochemical reactivities regardless of the sites in the large intestine. The transitional mucosa surrounding carcinoma tissues characteristically revealed decreased sulfation and neuraminidase‐induced GOS reactivity, suggesting the presence of NANA‐β Gal type terminals. The carcinoma tissues resembled the transitional mucosa in showing neuraminidase‐induced GOS reactivity, but differed from the latter in possessing intense UEA‐I reactivity. The present study indicated that the refined histochemical techniques recently developed were quite useful for understanding the histochemical reactivities correlating with sugar structure

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1985.tb01438.x

出版商:Blackwell Publishing Ltd    

年代:1985

数据来源: WILEY