摘要:

In situhybridization has been recognized as a powerful technique for localizing specific nucleic acids such as mRNA and viral DNA in individual cells. Forin situhybridization, the use of a non radioactive probe is considered superior to that of a radioactive one from viewpoints of resolution, probe stability and personal safety. Although various non‐radioactive labels are currently available, some interfere with the formation of hybrids and some increase steric hindrance and prevent penetration of the labeled nucleic acid probe into cells and tissues. Recently, we have developed a method using thymine thymine (TT) dimerized DNA as a nonradioactive probe. This is simple to make, since it dose not require separation of labeled DNA from unreacted labeling compounds, and T‐T dimer will not alter the chemical and physical nature of the probe. In this paper, we describe the tissue processing procedures that are suited for the TT dimerized DNA probes, and the recent new findings on methodological aspects, particularly the use of synthetic oligodeoxynucleotide probes. Acta Pathol Jpn 40: 793‐807,

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb02492.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Fiber types in human extraocular muscle (h‐EOM) were examined immunohistochemicaliy with antibodies against slow tonic (anti‐ALD) and slow twitch (anti‐SOL) myosins. Four types of muscle fiber in h EOM were distinguishable according to their reactivities with these antibodies. Groups 1 and 2 fibers reacted with both antibodies, group 1 fibers showing stronger reactivity than group 2 fibers with anti‐ALD. Group 3 fibers reacted only with anti‐SOL. Group 4 fibers did not react with either antibody. The latter were the most common, and were the main fibers in both the peripheral (outer orbital) and central zones of h EOM. The next most common were group 1 fibers, which were located mainly in the peripheral layer. Group 2 fibers were less common, but were the second most common type in the central layer. Group 3 fibers were only minor constituents. Multiple innervations were observed in some fibers of groups 1 and 2, and group 1 fibers were suggested to be slow tonic myofibers in h‐EOM. These specific immunohistochemical and physiological features of h‐EOM seem to be the basis of the low morbidity seen in the usual types of muscular dystrophy. Acta Pathol Jpn 40:

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb02493.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Intracytoplasmic lumina, each with a peripheral halo and spikes, a hitherto undescribedhistologicalstructure, were found in several human carcinomas. Similar structures found at the free surfaces of carcinoma cells, or those composed of several individual carcinoma cells, were designated extracytoplasmic lumina with a peripheral halo and spikes. Haloes were seen along the cytoplasm around the lumina, and the spikes penetrated into the cytoplasmic haloes. Transmission electron microscopy revealed that these spicular bodies were lumina equipped with microvilli containing an actin core. Spicular bodies were found in the following neoplasms; 22% of hepatocellular carcinomas (11/50), 35% of colonic adenocarcinomas (7/20), 31% of gastric adenocarcinomas (16/51). In contrast, no or only very occasional spicular bodies were found in thyroid (0/20) and mammary (1/22) adenocarcinomas. Although the diagnostic value of this structure is unknown at the present time, its identification in a carcinoma may be a histologic indicator of its endodermal nature. Acta Pathol Jpn 40: 815‐819, 199

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb02494.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Ninety‐five autopsy cases of chronic renal failure, which had or had not been treated by hemodialysis, were examined histologically and immunohistochemically for evidence of amyloid deposition in the intervertebral disks and posterior longitudinal ligaments of the spine, β2‐Microglobulin (β2M) amyloid was not present in non‐dialyzed patients with chronic renal failure. In cases showing β2M amyloid deposition, the shortest term of hemodialysis was 2 years and 5 months. The incidence of β2M amyloidosis tended to increase as the dialysis period was prolonged. An inverse correlation was present between dialysis period and age in 22 cases showing β2M amyloid deposition (correlation coefficient: –0.43, p<0.05), and β2M amyloidosis tended to occur earlier in elderly patients than in younger patients. This suggests that elderly patients are more susceptible to&M amyloidosis. β2M amyloid was absent in all of 8 cases of systemic lupus erythematosus which were treated by dialysis for periods ranging from 2 days to 12 years. In these patients, β2M amyloidosis may have been prevented by steroids, which had been administered for long periods in all cases. Another amyloid of unknown composition was also frequently present in the intervertebral disks and posterior longitudinal ligaments not only in patients with chronic renal failure but also control patients without chronic renal failure. This amyloid was immunohistologically negative for β2M, amyloid A protein, light chain kappa or lambda, prealbumin, and apolipoprotein All. Acta Pathol Jpn 4

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb02495.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

We present a rare case of submandibular gland carcinosarcoma occurring in a 45 year old male patient. His clinical history revealed that the carcinosarcoma had developed from a carcinoma ex mixed tumor in three years. In spite of repeated resection, intensive chemotherapy and irradiation, the tumor recurred and grew rapidly, and the patient died of hemothorax caused by rupture of a pulmonary metastatic tumor. The fourth recurrent tumor and autopsy specimens showed features of carcinosarcoma consisting of three tumor components, i.e., undifferentiated carcinoma, and chondrosarcomatous and osteosar‐comatous growth. The metastatic nodules in both lungs and pulmonary hilar lymph nodes showed the same pattern. Immunohistochemically, the chondrosarcomatous cells were positive for vimentin and S 100 protein, and for epithelial markers such as epithelial membrane antigen (EMA) and cytokeratin (MA 902). Undifferentiated carcinoma cells, on the other hand, were partially positive for muscle actin other than cytokeratin (KL 1). Ultrastructur‐ally, desmosome like structures were seen in the chondrosarcomatous cells. These findings suggest that the sarcomatous lesions might have originated from epithelial cells. Acta Pathol Jpn 40: 827 831, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb02496.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A case of ameloblastic odontosarcoma (ameloblastic fibro‐odontosarcoma) originating in the mandible is reported. The patient was a 23 year‐old Japanese male with diffuse swelling of the mandible from the left premolar to the ramus region. A biopsy specimen showed the histopath‐ological features of ameloblastic fibrosarcoma. Histologic examination of the surgical specimen revealed various amounts of dysplastic dentin and dentinal matrix which were closely associated with both mesenchymal and epithelial components throughout the lesion. In addition, a small amount of abortive enamel matrix, which was in close contact with ameloblastoid cells of epithelial nests, was found by extensive sampling of the resected specimen. Therefore the present case was diagnosed as ameloblastic odontosarcoma. After 2 years, the patient died of uncontrollable local recurrence and extension to the cranial base. Acta Pathol Jpn 40: 832 837,

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb02497.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A rare case of adenocarcinoma of the female urethra with alpha‐fetoprotein (AFP) positivity in a 52‐year‐old woman is reported. The tumor was papillary polypoid, localized in the posterior wall of the mid‐urethra and microscopically showed three histologic components. Upon immunostain‐ing and histochemical staining, the tumor was characterized by intestinal type cells positive for epithelial membrane antigen (EMA) and carcinoembryonic antigen (CEA), EMA negative and AFP positive columnar vacuolated cells and mainly EMA positive clear cells. On the basis of these features together with the known embryogenesis of the urethra, an endodermal origin of the tumor is suggested, possibly arising from the reserve or stem cells in the urethral mucosa. This case and its immunohistochemical features are quite unique and the histologic combination is meaningful when considering the oncogenesis and histogenesis of urethral tumors. Acta Pathol Jpn 40: 838

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb02498.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A case of malignant mixed mullerian tumor of the ovary in a 57 year old woman is reported along with the results of an immunohistochemical study. The tumor, measuring 16·10·9cm, was composed predominantly of adenocarcinoma with a smaller amount of anaplastic carcinoma as an epithelial component and chondrosarcoma, liposarcoma, fibrosarcoma and rhabdomyoblasts as mesenchymal elements. Immunohistochemistry using paraffin sections demonstrated cytokeratin (CK) and epithelial membrane antigen (EMA), generally regarded as epithelial markers, not only in the epithelial component but also in chondrosarcoma cells. Vimentin and desmin, generally regarded as mesenchymal markers, were exhibited partly in carcinoma cells as well as in mesenchymal elements. Positive staining for S 100 protein was obtained not only in chondrosarcoma and liposarcoma cells, but also partly in adenocarcinoma cells. This intricate immunohistochemical picture reflected the histologic findings. It is noteworthy that both carcinoma cells and chondrosarcoma cells demonstrated simultaneous expression of CK, EMA, vimentin, desmin and S 100 protein. This somewhat unusual antigen expression by tumor cells may indicate a change in the nature of tumor cells due to microenvironmental factors. Acta Pathol Jpn 40: 845 850, 199

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb02499.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Eosinophilic granuloma confined to the lymph node of a 25‐year‐old Japanese woman is reported. She presented with a low‐grade fever and pain in the left cervical region. A finger‐tip‐sized cervical lymph node was excised for histological study. Microscopically, the normal architecture of the lymph node was distorted, and histiocytoid cells had proliferated mainly in the sinus. Numerous eosinophils and an appreciable number of large multinucleated cells were also observed. Ultrastructurally, Birbeck granules were found in the cytoplasm of the histiocytoid cells. Immunohistochemically, the proliferating cells and multinucleated cells were found to be positive for S 100 protein but negative for lysozyme in the cytoplasm. The patient has been well without recurrence for 10 months after the lymph node biopsy. Acta Pathol Jpn 40: 851

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb02500.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Primary intraosseous glomus tumor is rare and may occur in the medullary cavity of bone. A 22 year old woman with a one year history of spontaneous and persistent sacral pain was found to have an intraosseous glomus tumor in this unusual location. Curettage was followed by complete relief from pain. This case of glomus tumor originating in the sacrum is thought to be the first reported in the world literature. Acta Pathol Jpn 40: 856‐859, 199

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb02501.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY