摘要:

Destruction of Methylcholanthrene‐induced mouse sarcomas in tissue culture was mediated by lymphoid cells from syngeneic mice immunized with the same tumor. Serum or ascitic fluid from the same mouse had no effect. Complement was not required for the reaction. Target tumor cell monolayers growing on Millipore filters were destroyed by immune lymphoid cells added on the other side of the filter.The results suggest that some diffusible substance(s) elaborated by immune lymphoid cells plays an important role in ‘cell‐mediated’ immune reactions in the syngeneic tumor‐ho

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1969.tb00088.x

出版商:Blackwell Publishing Ltd    

年代:1969

数据来源: WILEY

摘要:

Yoshida ascites hepatoma AH 13 cells were investigated by electron‐microscopy and by electron‐histochemistry combined with the metal precipitation technique for acid phosphatase, especially giving attention to the changes of lysosome system associated with enzyme activity.Abundant deposition of intracellular glycogen appeared not only in form of rosettes, but also more frequently in monoparticulate fashion. Acid phosphatase activity was detectable in vacuolated lysosomes, but their intracellular distribution was a little more random in contrast with that of pericanalicular lysosomes of normal liver cells. In addition, the tumor cell lysosomes appeared slightly irregular in size compared with those of hepatocytes. So‐called myelin figures which were occasionally encountered In the tumor cell cytoplasm were also stainable for acid phosphatase. It is worth mentioning that the Golgi apparatus of AH 13 cells was also acid phosphatase positive contrary to the negative finding in Golgi apparatus of normal liver cells.Glycogen‐containing lysosomes (glycogenosomes) were often found within or in the vicinity of cytoplasmic glycogen areas. The engulfed glycogen particles sometimes lost their granularity and high density, and became amorphous in appearance suggesting their lytic process within the organelle. In quite rare cases, glycogenosome membranes were stained for acid phosphatase.The metabolism in lysosome system of AH 13 cells seems to be considerably different from that of normal live

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1969.tb00089.x

出版商:Blackwell Publishing Ltd    

年代:1969

数据来源: WILEY

摘要:

Changes produced by intracerebral injection of alum phosphate and by administration of lead carbonate via maternal milk in the cerebellum of rats and rabbits were examined by light and electron microscopy.Two different types of degeneration of Purkinje cells were described. The first type of change was of Alzheimer's neurotibrillary change and was found in the cerebellum of alum‐treated rabbits. It appeared as a focal cytoplasmic clearing of the Purkinje cell bodies and their major dendrites. The areas of clearing displayed marked argentophilia.The second type of change in Purkinje cells was calcification of Purkinje cell dendrites. It was found both in animals with alum and lead encephalopathy. In routinely stained sections, it appeared as collections of tiny granules, rounded and club‐shaped bodies, or it had a bushy appearance (Pentschew) in the molecular layer. By electron microscopy this formation appeared as dense aggregates. The agglomerations were found to consist of small dense spheres, amorphous oval bodies and occasionally of closely packed needle‐shaped crystallines. It was suggested that calcification starts from the most peripheral portion of the Purkinje cell dendrites and extends later over the large areas of the cytoplasm. Abnormalities were also found in the synaptic connections of the Purkinje cell dendrites with axon terminals of the surrounding neu

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1969.tb00090.x

出版商:Blackwell Publishing Ltd    

年代:1969

数据来源: WILEY

摘要:

Systematic observation of arteriosclerosis by electron microscope was made on small arteries and arterioles of the stomach which were respected from 5 hypertensive cases and 6 normotensive cases who underwent surgery for gastric carcinoma or ulcer and which were fixed by perfusion with glutaraldehyde. Concurrently, three rabbits were used for animal experiments. Upon placing electrodes in the gastric wall of the rabbits, electric stimulation with 2.0 volt D.C. was repeated intermittently during the period of 5 to 12 days.Repeated contraction of vessels resulted in crush‐up effect of smooth muscle cells beneath the internal elastic lamina and in transition of irregularly minced cytoplasms into small osmophilic particles. At the same time dark cells appeared among the smooth muscle cells in the vicinity. The same findings were frequently noted in the lesions of gastric artery of man particularly in the hypertensive group. It was apparent that repeated angiospasm is an important factor for the histogenesis of sclerotic vascular lesion.The cells proliferating beneath the endothelial cells in arteriosclerosis were smooth muscle cells, and the direction of the proliferated smooth muscle cell layers did not necessarily conform with that of the media. They originated in the media and penetrated through the fenestrae of the internal elastic lamina.Fibrinoid necrosis and hyalinous thickening of arterioles were observed fairly frequently in the hypertensive group but always accompanied with intensive irregular atrophy or disappearance of the medial smooth muscle cells.Vascular lesions with initial thickening showed formation of new elastic lamina most of which was elastic fibers consisting of microfibrillar components. The additional formation of elastic fibers was particularly strong in the hypertensive group as the whol

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1969.tb00091.x

出版商:Blackwell Publishing Ltd    

年代:1969

数据来源: WILEY

摘要:

An autopsy case of Rubinstein‐Taybi's syndrome is described. Since the mental retardation is considered to be a part of the syndrome, central nervous system was carefully examined, illustrating a moderate degree of the cerebral cortical and callosal hypoplasia and diffusely distrlbuted neuronal anoxic changes possibly associated with cardio‐pulmonary insufficiency. As far as the pathognomony of the facio‐osseal deformities, no convinciable causative factors have been obt

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1969.tb00092.x

出版商:Blackwell Publishing Ltd    

年代:1969

数据来源: WILEY

摘要:

A case of generalized cytomegalic inclusion disease in an adult demonstrating clinical features of cytomegalovirus mononucleosis was presented. This condition was proceeded by serious involvement of systemic lymph nodes.Twenty‐four cases of adult cytomegalic inclusion disease in Japan were analyzed, and the type of involvement, sexual incidence, site of involvement and predisposing conditions were discusse

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1969.tb00093.x

出版商:Blackwell Publishing Ltd    

年代:1969

数据来源: WILEY

摘要:

Two autopsy cases of Letterer‐Siwe disease and a bone lesion of eosinophilic granuloma obtained at surgical operation have been presented.Electron microscopic examination of the skin lesion of one case of Letterer‐Siwe disease revealed that the histiocytes accumulating in the dermis contained characteristic Langerhans cell granules. The number of granules was variable due probably to cell maturation or activity. Similar histiocytes containing the Langerhans cell granules were observed in the bone lesion of eosinophilic granuloma.From this, it is assumed that the histocytes in these disorders have a common functional property.In addition, the possible origin of the epidermal Langerhans cell was also discussed in relation to these findi

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1969.tb00094.x

出版商:Blackwell Publishing Ltd    

年代:1969

数据来源: WILEY

摘要:

A case report of a reticulum cell sarcoma of the thyroid is presented. The replacement of the thyroid except for the left upper lobe by a solid tumor mass suggested the thyroid as a primary site. The tumor involved the surrounding strap muscles and trachea. Lymph nodes were only minimally involved. In a review of 152 autopsy cases of malignant lymphoma from the files of ABCC in Nagasaki and Hiroshima, the thyroid showed focal interstitial infiltrations in 20 cases: reticulum cell sarcoma in 9, lymphosarcoma in 9 and Hodgkin's disease in 2. In only one case was a solid tumor (0.3 cm) found in the thyroid. The diagnostic features of primary reticulum cell sarcoma of the thyroid and its distinguishing features from the small cell carcinoma are discussed.The diagnosis of malignant lymphoma of the thyroid must be based upon the gross and histological features. The clinical history and the anatomic distribution of this disease may also be of diagnostic aid in the final diagnosis.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1969.tb00095.x

出版商:Blackwell Publishing Ltd    

年代:1969

数据来源: WILEY

摘要:

A typical case of the Duchennetype of progressive muscular dystrophy with autopsy findings was presented. Changes in the myocardial and smooth muscle of many organs were found, and the skeletal muscles also revealed florid changes.Histopathological examination of the skeletal muscle was made in detail through light and electron microscopic observation.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1969.tb00096.x

出版商:Blackwell Publishing Ltd    

年代:1969

数据来源: WILEY