摘要:

To examine the effect of colchicine on ethionine induced fatty liver, adult female rats were starved overnight and then injected i.p. with 1 g kg ethionine at 11th hour of fasting; then a half of the rats were also injected i.p. with 2.5 mg kg colchicine twice at 3 and 6 h after the single administration of ethionine. Similarly, fasted control rats were injected i.p. with vehicle alone at the above times. All of the rats were sacrificed after a 20 h fast, and the hepatocytes in periportal areas were observed ultra‐structurally. In addition, total lipids in the liver tissue were extracted and determined biochemically. Although similar significant increases of triglyceride were observed in the liver tissue of all ethionine‐injected rats, the hapatocytes in the group treated with both chemicals had fewer cytoplasmic fat globules (CFG) than those in the group treated with ethionine only. On the other hand, the diameters of markedly increased membrane‐bound lipid particles (MLP) in the double treated group were distributed mainly in the range 0.2–0.4 μm, compared with those (0.1‐0.2 μm) in the other groups. These findings indicate that colchicine inhibits the development of CFG in ethionine injured hapatocytes. Acta Pathol Jpn 39: 28

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02437.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

In order to investigate whether mesangial transport by glomeruli is delayed in ddY mice pretreated with sheep anti type IV collagen serum, the mice were administered an overload of human IgA myeloma serum. Non pretreated ddY mice used as controls and both experimental and control BALB/c mice were also processed in a similar manner. The intensities of mesangial deposition of human IgA were examined periodically and were found to correlate well with deposition of mouse IgA. Both mouse and human IgAs showed a gradual increase for up to 8 experimental weeks. In the control young ddY mice, however, the overloaded mesangial human IgA quickly disappeared, presenting no appreciable mesangial deposition of autologous IgA. In sharp contrast, both the experimental and control BALB/c mice showed an initially prolonged and rather heavy mesangial deposition of human IgA, followed by a gradual decrease and somewhat light mesangial deposition of autologous mouse IgA. These results obtained using experimental ddY mice appear to confirm the possibility that non immunological local trapping, due to retardation of mesangial transport function, causes mesangial deposition of autologous mouse IgA in this particular strain. Acta Pathol Jpn 39: 289 295, 1989.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02438.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Computer imaging analysis was used for quantitative evaluation of the extents, amounts and distributions of glomerular extracellular components, such as the 7S and NC 1 domains of type IV collagen, laminin (LN), fibronectin (FN) and IgA, in glomeruli from patients with IgA nephropathy. Renal biopsy specimens from 13 patients with IgA nephropathy were incubated with mouse monoclonal antibodies against the FN or non collagenous (NC 1) domain of type IV collagen or polyclonal antiserum against the LN or 7S domain of human type IV collagen, and then stained with appropriate dilutions of FITC labeled anti mouse Ig antisera. Marked staining of the 7S or NC 1 domain of type IV collagen, LN or FN was detected in the glomerular capillary walls and/or mesangial areas in patients with IgA nephropathy. In particular, a prominent increase of FN was observed in the subendothelial regions of glomerular capillary walls, i.e. mesangial interposition, in the moderate or advanced stage of IgA nephropathy. Therefore, computer imaging analysis was shown to be useful for the quantitative determination of such components distributed in glomeruli from patients with IgA nephropathy. Acta Pathol Jpn 39: 296 305, 1989.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02439.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Squamous differentiation of thyroid carcinoma was studied clinicopathologically and immunohistochemically in 29 autopsy cases. Tumor cell nests with squamous differentiation (CNSD), which histologically resembled squamous cell carcinoma, were found in 6 cases (20.7%). All of these 6 cases with CNSD had areas of undifferentiated carcinoma, representing 31.6% of 19 cases with undifferentiated carcinoma, and all but one case also showed coexisting papillary carcinoma. The CNSD were histologically associated with undifferentiated carcinoma in 5 cases, and with papillary carcinoma in one case; the CNSD were occasionally intermingled with these types of carcinoma, and there were findings suggesting a histological transition between the CNSD and undifferentiated carcinoma or papillary carcinoma. Immunohistochemistry revealed that all the CNSD were reactive with antibodies for keratin and vimentin, whereas thyroglobulin and desmin were not expressed. It was concluded that the CNSD examined here were most probably due to extensive squamous differentiation (squamous metaplasia) in undifferentiated carcinoma and papillary carcinoma. In addition, the present results may explain the fact that cases diagnosed solely as squamous cell carcinoma sometimes show a prognosis similar to that of undifferentiated carcinoma, and may well represent extensive squamous differentiation in such tumors rather than true squamous cell carcinoma of the thyroid. Acta Pathol Jpn 39: 306 312, 1989.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02440.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The correlation between the expression of blood group substance (BGS) and the host's immune response was studied in 23 samples of human colorectal carcinoma tissue. Several monoclonal antibodies against lymphocyte subsets and histiocytes were used for immunostaining of frozen sections, and MoAbs against BGS (A, B, H) for paraffin sections. In proximal colon cancers (10 cases), the cancer tissues showing expression of BGS revealed a greater increase in cells reactive for 0KT11, Leu‐7, and Leu M3 than those with deletion of BGS. However, in distal large bowel cancers (13 cases), there was no significant difference between the cancer tissues without BGS expression and those with BGS re expression. In all locations of the large bowel, cancer tissues showing expression of incompatible antigen revealed a greater reduction of reactive cells than those with expression of compatible antigen. These results suggest that there are disparities in the host's immune response to proximal colon and distal bowel carcinoma, and that factors other than BGS (A, B, H) might influence the immune response in cases with expression of incompatible antigen. Acta Pathol Jpn 39: 313∼320, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02441.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The histogenesis of clear cell sarcoma was investigated by immunohistochemical examination of five tumors (two melanotic and three amelanotic) and electron microscopic examination of two of these tumors (one melanotic and one amelanotic). Melanin production was observed histologically in two of the tumors. The cytoplasm of ceils in both types of tumor contained various numbers of melanosomes. Melanoma‐specific antibody (HMB‐45), anti S 100 protein, and anti vimentin antibodies gave positive reactions in four tumors, while all tumors showed Leu 7 immunoreactivity. No cytokeratin or epithelial membrane antigen (EMA) was detected immunohisto‐chemically in any tumor. The immunoreactivity of this type of tumor with HMB‐45 antibody strongly suggests melanocytic differentiation rather than schwannian or synovial differentiation. The reaction of the cells of one tumor with only Leu‐7 indicates the existence of undifferentiated clear cell sarcoma of neuroectodermal origin that does not show definite melanocytic differentiation. Acta Pathol Jpn 39: 321

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02442.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

A case of pancreatic tumor with features of both duct and islet cell components was found incidentally at autopsy in a 76 year old male who had died of intrahepatic cholan‐giocarcinoma. The tumor, measuring about l.0cm in diameter, was located in the pancreatic tail. The tumor was composed of two distinct cell populations, islet cells and duct cells. Immunocytochemically, nearly all of the former cells were positive for insulin but negative for cytokeratin, carcinoembryonic antigen (CEA) and mucin, while the latter were positive for cytokeratin, CEA and mucin but negative for insulin. Additionally, a majority of the tumor cells that had formed islet‐like structures were positive for neuron specific enolase (NSE), whereas NSE‐positive cells were found only rarely in duct components. Electron microscopy confirmed the presence of two cell populations. Simultaneous occurrence of duct and islet cell components in a single pancreatic tumor indicates an intimate histogenetic relationship between pancreatic endocrine and duct cells. Acta Pathol Jpn 39: 328 335,

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02443.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

An autopsy case report of leiomyosarcoma of the thoracic aorta is presented. The patient was a 75‐year old Japanese woman who complained of hypertension, melena and lumbago. The autopsy revealed severe occlusion of the thoracic aorta due to the tumor. Histologically the tumor consisted of fascicular proliferation of atypical fusiform cells. Metastases were seen in the bilateral adrenal glands, pancreatic tail and bones (vertebrae and femur). At the distal portion of the aortic tumor, there was a secondary dissecting aneurysm. Acta Pathol Jpn 39: 336 341, 198

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02444.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

An autopsy case of massive renal hemorrhage in a 49 year old male who had undergone maintenance hemodialysis for 8 years, is reported. No bleeding tendency had been noticed, and blood pressure had been reduced to within the normal range. Histological investigation with semiserial sections revealed that hemorrhage had occurred in four arteries, corresponding to the interlobar, arcuate, and interlobular levels, which existed in the same ruptured cyst wall. Acute dissection had occurred in two of the four arteries, leading to rupture of the cyst; this led to destruction of the remaining arteries. Both kidneys, which were markedly shrunken and had numerous cysts in the cortex and medulla, fell into the category of acquired cystic renal disease of long term hemodialysis. It was suspected that renal vascular change during hemodialysis, mechanical factors compressing the protruding artery in the cyst with scanty renal interstitium, and relatively radical hemodynamic changes during dialysis had contributed to the hemorrhage. Acta Pathol Jpn 39: 342∼348, 198

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02445.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY