摘要:

The effects of long term administration of synthetic salmon calcitonin (TZ‐CT) on thyroid C cells of rats were evaluated immunohistochemically and electron microscopically. Low dose (0.075, 0.75 and 7.5 IU/kg/day) administration caused no significant changes in the C cell population, whereas high doses (30 and 120 IU kg day) caused C cell suppression. The area ratio of C cells to total cells in the thyroid was measured on sections immunoperoxidase stained for calcitonin. The average C cell ratio was compared among the experimental groups. This mor‐phometric analysis of the high‐dose and control groups confirmed the differences in C cell populations statistically (30 IU/kg vs. control, pgO.05; 120 IU/kg vs. control, p ≦ 0.05). The suppressive effect of TZ CT on C cells was reversible after cessation of administration for one month. Electron microscopic examination on the C cells in the high dose groups revealed no particular ultrastructural differences. These results may suggest a negative feedback mechanism on C cells by exogenous CT. Acta Pathol Jpn 39: 545‐

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02482.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

A study was conducted to investigate nephritogenic tubular basement membrane antigens common to human and rat kidneys. Brown Norway (BN) rats were immunized with human renal basement membrane in complete Freund's adjuvant simultaneously withBordetella pertussisvaccine. The immunized rats developed polyuria and increased levels of serum creatinine one week after the second immunization. Renal histology at this time revealed marked, acute tubulointerstitial nephritis with linear deposition of IgG and C, along the tubular basement membrane and Bowman's capsule, but not along the glomerular basement membrane. Rats with this tubulointerstitial nephritis rapidly developed antibodies against renal antigens from normal BN rats such as tubular basement membrane and proximal tubule brush border, however antibodies to glomerular basement membrane appeared later. Western blotting using the same rat sera detected a 145 kDa antigen from 8 M ufea solubilized human renal basement membrane and 120‐kDa, 135 kDa and 145 kDa antigens from 8M urea solubinzed BN rat renal basement membrane. This suggests that renal basement membranes of human and rat origin have common antigens involved in the pathogenesis of tubulointerstitial nephritis. Acta Pathol Jpn 39: 551 557, 198

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02483.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Changes in glomerular anionic sites were examined mor‐phometrically in nephrotic rats receiving daily subcutaneous injections of puromycin aminonucleoside (PAN). The high iron diamine thiocarbohydrazide silver proteinate mothod showed that anionic sites composed of heparan sulfate proteoglycans formed a continuous band within the lamina rara externa of the glomerular basement membrane (GBM) in control rats. Only one day after the first injection of PAN, anionic site loss was already detectable, preceding the morphological changes in the epithelial cells. The number and size of the anionic sites decreased greatly between days 7 and 10, when urinary protein excretion began to appear. The number of anionic sites in the paramesangial regions (epithelial side of the mesangium) was slightly more reduced than that in the capillary walls. These results suggested that PAN directly injured the glomerular anionic sites and increased the permeability of the glomerulus to macromolecules. However, there was no complete correlation between the number of lost anionic sites and the level of urinary protein excretion. Thus protein excretion into the urinary space may be related not only to the loss of subepithelial anionic sites but also to dysfunction of the protein absorption mechanism in epithelial cells due to excessive permeation of macromolecules through the GBM. Acta Pathol Jpn 39: 558‐565, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02484.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The degree of DNA polyploidy of cardiac muscle cells was investigated by cytofluorometry in 4 cases of progressive muscular dystrophy (DMP) and 2 cases of myotonic dystrophy (DM), and the results were compared with those for non dystrophic hearts of normal or increased weight. The heart muscle cells from all patients with these forms of dystrophy showed marked nuclear DNA hyperploidy reaching 16c, irrespective of cardiac hypertrophy or atrophy. In the non dystrophic group, DNA hypertrophy corresponding to that of dystrophy was only detected in cases of marked cardiac hypertrophy exceeding a weight of 400 g. The wasting of the respiratory musculature, deformity of the thorax and cardiac muscular atrophy appeared to be the principal factors causing DNA polyploidy in patients with muscular dystrophy. Acta Pathol Jpn 39: 566 572,1989.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02485.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

We carried out a histologic, immunohistochemical and ultrastructural study of 19 tumors, previously classified as Ewing's sarcoma of the bone (ESB). The tumors were categorized histologically into four groups according to the presence or absence and the distribution of Homer‐Wright rosettes, together with the immunohistochemical and ultrastructural features: 1) neuroectodermal tumor of bone (NTB) with diffusely dispersed rosettes (4 cases), 2) conventional ESB totally lacking the rosettes (9 cases), 3) intermediate type tumor with characteristics of both NTB and ESB (4 cases), and 4) spindle cell type tumor with focal rosettes (2 cases). Ultrastructurally, NTB contained neurotubules and neurosecretory granules, whereas ESB consisted of only undifferentiated cells. Immunoreactivity with antineural antibodies, such as Leu 7, and those against synaptophysin, NSE, neurofilament, and chromo‐granin, was detected in the majority of NTB, in intermediate and spindle cell‐type tumors and in two of the nine cases of ESB. In view of these findings, we propose that use of the term “neuroectodermal tumor of the bone” should be restricted to tumors with an extensive distribution of rosettes, and that those without unequivocal rosettes should be classified as ESB, although ESB may be the most undifferentiated form of NTB and some overlap may occur between the two tumor types. Acta Pathol Jpn 39: 573

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02486.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The correlation between gallbladder stones (gallstones) and various gallbladder diseases was examined. The incidence of gallstones was 47.1% in cases of nonneoplastic epithelial polyp, 48.0% in adenoma, and 59.1% in adenocarcinoma. There was no relation between the incidence of gallstones and sex. The incidence of gallstones in cancer cases showed no difference among histological gradings. Non neoplastic epithelial polyps, adenomas and carcinomas were divided into metaplastic type and non metaplastic type based on the presence or absence of metaplastic changes. Comparison of the incidence of gallstones showed that cases of metaplastic type polyp and adenoma had a higher incidence of gallstones than non metaplastic type polyp and adenoma. In the cases of cancer, no differences were observed in the incidence of gallstones between metaplastic type and non metaplastic type carcinomas. However, comparison among early carcinomas showed that metaplastic type carcinoma had a significantly higher incidence of gallstones than the non metaplastic type. These results suggest that gallstones may play an important role in the histogenesis of gallbladder diseases of the metaplastic type. Acta Pathol Jpn 39: 582 585, 1989.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02487.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The histopathology of the liver in idiopathic portal hypertension (IPH) associated with autoimmune disease (15 cases), was examined and compared with that of IPH without autoimmune disease (31 cases). It was found that hepatic histopathology was heterogeneous in the cases with autoimmune disease. That is, the hepatic histopathology in 7 cases was similar to that of classic IPH without autoimmune disease, and the remaining 8 cases disclosed unusual lesions such as focal non suppurative cholangitis, nodular parenchymal hyperplasia, moderate portal inflammation, and intrahepatic ductopenia. These unusual lesions, which frequently coexisted in the same case, were not typical ones for making other diagnoses such as primary biliary cirrhosis or nodular regenerative hyperplasia of the liver. These findings suggest that unusual histologic lesions in the livers of IPH patients with autoimmune disease may represent an accentuated immunologic reaction inherent in IPH, or that such cases may be an abortive or incomplete form of primary biliary cirrhosis or nodular regenerative hyperplasia of the liver. Acta Pathol Jpn 39: 586‐592, 198

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02488.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Secretory carcinoma and mucinous carcinoma were found to coexist in the breast of a 67 year old post menopausal woman, although the tumors were separated by a thin fibrous septum. Histochemically, intra‐ and extracellular secretory materials in both carcinomas were strongly positive for alcian blue, PAS and mucicarmine staining, but immunohistochemically negative for α‐ lactalbumin and CEA. Membrane‐bound intracytoplasmic vacuoles showing emiocytosis were observed in both the secretory and mucinous carcinomas by electron microscopy. No differences were observed between the tumor cells of secretory carcinoma and those of mucinous carcinoma by histochemistry, immunohistochemistry and electron microscopy. However, there were definite statistically significant differences in the results of morphometry of tumor cell nuclei. Secretory carcinoma is considered to be an anaplastic variant type of mucinous carcinoma. Acta Pathol Jpn 39: 593 598

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02489.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

We present an autopsy report on a 14‐year‐old girl with mitochondrial myopathy, encephalopathy, lactic acidosis and stroke‐like episodes (MELAS), placing emphasis on the mitochondrial enzymatic histochemistry of the 3 types skeletal muscle and cardiomyocytes. Generalized muscular atrophy, cardiac hypertrophy, cerebral cortical laminar necrosis, basal ganglia calcification and liver steatosis were observed. In the skeletal muscles, modified Gomori's trichrome staining demonstrated scattered ragged red fibers, and histochemical staining for mitochondrial enzymes showed intense positivity in the subsarcolemmai zones of some muscle fibers. Some of the hypertrophic cardiomyocytes also showed a ragged red appearance with the modified Gomori's trichrome stain. Histochemical staining for mitochondrial enzymes showed patchy loss of enzymatic activity in the myocardium. Electron microscopically, extreme accumulation of enlarged mitochondria and severe loss of myofibrils was observed in both skeletal muscle fibers and cardiomyocytes. The arteriolar smooth muscle cells also showed a mild increase in mitochondria. Acta Pathol Jpn 39: 599 606,

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02490.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

We report a case of primary epithelioid hemangioendothelioma of the liver occurring in a patient with primary biliary cirrhosis, stage III. The hepatic tumor was found incidentally by imaging techniques and was surgically resected under a tentative diagnosis of metastatic carcinoma. The resected tumor (1.8±1.6 cm) showed Wtcal histologic features of epithelioid hemangioendd jelioma. The tumor cells were positive for factor VIII related antigen and were stained withUlex europaeuslectin I. Ultrastruc‐turally, the tumor cells showed cytoplasmic vacuoles, tight junctions, basal lamina, pinocytotic vesicles, bundles of thin filaments (approximately 10 nm in diameter) and Weibel Palade bodies. The non tumorous part of the liver showed features of primary biliary cirrhosis, stage III. This is the first reported case of epithelioid hemangioendothelioma occurring in a liver with primary biliary cirrhosis. Acta Pathol Jpn 39: 607 611, 19

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1989.tb02491.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY