摘要:

To elucidate the relationship between cell proliferation and structural remodeling in pulmonary fibrosis, light and electron microscopy with immunohistochemistry for bromo‐deoxyuridine (BrdU) and morphometry for BrdU positive cells were performed following a single intratracheal instillation of bleomycin in rats. The results showed that terminal bronchiolar epithelial cells, Type II alveolar epithelial cells and interstitial cells began to proliferate 2 days after the injury. Then each cell type showed a different style of proliferation. Interstitial cells which were located in the interstitium and migrated into intraalveolar spaces proliferated, then produced intra‐alveolar fibrosis. Terminal bronchiolar epithelial cells proliferated rapidly and formed alveolar bronchiolization with squamous metaplasia in areas where alveoli were severely damaged and intraalveolar fibrosis was formed, and thereafter the proliferation ceased within 2 weeks. The degree of proliferation of type II alveolar epithelial cells and interstitial cells was rather slight, but continued constantly untill the later stage. In addition, intraalveolar macrophages were BrdU positive from an early stage. Endothelial cell proliferation was observed in small vessels and alveolar capillaries at 1 week after bleomycin instillation. Rapidly proliferating bronchiolar epithelial cells which formed alveolar bronchiolization with squamous metaplasia were important in preventing the progress of intraalveolar fibrosis, because the proliferation of type II alveolar epithelial cells was limi

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01556.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

To evaluate the pathogenetic roles of immune complexes and alveolar hyaline membrane in idiopathic interstitial pneumonia (IIP), immunohistological and ultrastructural studies of the kidney and lung were performed in 23 cases of IIP, 19 cases of autoimmune diseases, 17 cases of interstitial pneumonia other than IIP, and 11 cases of bronchopneumonia as a control group. None of the cases of IIP or interstitial pneumonia other than IIP showed immune complexes in the alveolar and glomerular capillary walls. On the other hand, one case of SLE was positive for IgG and components of complement along the alveolar and glomerular capillary walls. The alveolar hyaline membrane in the present cases revealed immunoglobulins as well as components of complement, which were poorly soluble in chaotropic solution or acidic buffer. These results indicate that circulating immune complexes play a minor role in the pathogenesis of IIP and other types of interstitial pneumonia, and that there is no relationship between immune complex deposition in alveoli and the alveolar hyaline membrane. It is necessary to further investigate factors other than immune complexes involved in alveolar tissue damage and to clarify the significance of the hyaline membrane in the processes occurring from acute changes to pulmonary fibrosis in IIP.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01557.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

In 22 cases of rheumatoid arthritis (RA), including 4 cases of malignant RA (MRA), reactive proliferative lymph node lesions were studied clinicopathologically and immunohis‐tochemically. This series included 5 males and 17 females. The period between disease onset and lymph node biopsy ranged from 3 months to 41 years. Generalized lymphadenopathy was noted in 13 cases and constitutional symptoms in 8. The histological findings characteristic of RA were 1) follicular hyperplasia with active germinal centers and 2) polyclonal plasma cell infiltration in the interfollicular area. Studies of intracytoplasmic immunoglobulin showed that γ‐heavy chain‐expressing plasma cells were a major component in the interfollicular area in 17 RA cases. However, in 4 MRA cases, a prominent increase of μ chain‐expressing plasma cells was recognized in the same area. In the 3 cases for which fresh tissue sections were stained with monoclonal antibodies against lymphocytes, we found that the majority of T cells in the interfollicular area had helper/inducer markers. The identical locations of the T cell population and plasma cells indicated that both played a role in the proliferation and/or differentiation of B cells in lymph no

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01558.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Senile changes in the human olfactory bulb were studied histopathologically in 133 individuals ranging in age from 40 to 91 years with a mean age of 64.3 years. Neurofibrillary tangles (NFTs) in the olfactory bulb were observed in 47 subjects aged over 50 years. The frequency of NFTs was 35.3% (47/133) in total and 40.5% (47/116) among subjects 50 years of age or older. The frequency of individuals with NFTs increased linearly with age. Senile plaques (SPs) were observed in 5 individuals over 60 years old at a frequency of 3.8% (5/133) in total and 5.7% (5/88) among individuals 60 years old or more. Granulovacuolar degeneration was rarely seen, and amyloid angiopathy was not recognized. Senile changes in the hippocampus and parahippocampal gyrus were also examined. In some cases, the frequencies of NFTs and SPs in the hippocampal region were not identical to those in the olfactory bulb. However, it was not apparent whether senile changes first appear in the olfactory bulb or in the hippocampal region.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01559.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Carcinoembryonic antigen (CEA) and nonspecific cross‐reacting antigen (NCA) were studied immunohisto‐chemically in formalin fixed, paraffin embedded tissues of 73 cases of medullary carcinoma of the thyroid (MTC) using 2 polyclonal antibodies (CEA antisera cross‐reactive with or without NCA), 3 monoclonal antibodies recognizing epitopes only on CEA, and one monoclonal antibody against NCA. The staining patterns of the 5 antibodies against CEA in MTCs were not different, and they reacted with 86.3% of all cases. With regard to the effects of fixatives on the staining patterns, samples fixed with formalin or 4% paraformaldehyde demonstrated CEA immunoreactivity in both the cell membrane and cytoplasm. In Bouin fixed tissue, the immunoreactivity was predominant on the cell membrane, whereas cytoplasmic positivity predominated in alcohol fixed specimens. Thus the difference in fixatives used in previous studies does not appear to be a major reason for the difference in the reported incidence of CEA positive MTCs. It is concluded that CEA is still a useful tumor marker for MTC and that it is detectable only in thyroid tumors originating from C cells, as seen in our series. The epitope defined by monoclonal antibody F106 88, present only on NCA, was found in 42.5% of all cases (49.2% of CEA positive MTCs). The NCA immunoreactivity was located in the tumor cell cytoplasm as globular aggregates, which were also labeled fo

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01560.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

We present five cases of adenomatous hyperplasia (AH) containing minute hepatocellular carcinoma (HCC) in cirrhotic liver. All the patients were Japanese, four males and one female, ranging in age from 60 to 80 years. Two of the specimens were obtained at surgery and the others at autopsy. The AH specimens ranged from 2.0 to 3.0 cm in diameter, and the maximum diameter of HCC foci in the AH was 2.0 cm. Histologically, apart from the HCC foci, the AH specimens showed intrinsic atypia, suggesting preneoplastic change. These features included an increase of both cellularity and the nucleo cytoplasmic ratio, distortion of cord structure and pseudoacinar formation. Ail of the AH specimens contained typical portal triads. Details of diagnostic imaging were also obtained in four cases. The findings of the present study support the possibility that AH with intrinsic atypia is a preneoplastic lesion of HCC. The sequence of “adenomatous hyperplasia with intrinsic atypia HCC foci” would thus represent part of the early phase of hepatocarcinogenesis in hum

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01561.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

We report a case of acinic cell carcinoma of the parotid gland with neuroendocrine differentiation. Light microscopically, the tumor appeared as clear cell‐type acinic cell carcinoma. In addition, the tumor showed neurosecretory features such as Grimelius positivity and the presence of neurosecretory granules by electron microscopy. We suggest that a tumor cell arising from a stem cell can show simultaneous differentiation to both neuroendocrine and acinic cell

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01562.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Cardiac dysfunction and ECG abnormalities were demonstrated in a 51 year old woman suffering from secondary hemochromatosis in sideroblastic anemia. Hemosiderin deposition in vacuolized and disarrayed myocytes was disclosed by microscopic examination of the first biopsy specimen of endomyocardial tissue. Three months after administration of deferoxamine mesylate, an iron chelating agent, the clinical findings were improved. The second endomyocardial biopsy revealed marked depletion of hemosiderin deposition in the myocytes, and improvement of myocyte vacuolization and disarray. Ultrastructurally, the highly electron dense granules in the myocytes were also decreased in number and density. X ray microanalysis revealed a prominent peak of Fe in the granules. In a liver specimen obtained by needle biopsy 5 months after the second endomyocardial biopsy, marked hemosiderin deposition still remained.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01563.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

We report an unusual case of malignant mixed mesodermal tumor of the uterine corpus associated with various symptoms related to overproduction of catecholamine by the tumor cells. Histologically, the tumor was dominated by carcinomatous epithelium with foci of malignant mesen‐chyma. The type of epithelium was endometrioid with papillary adenocarcinomas containing foci of malignant squamous epithelium. The malignant mesenchyma consisted mainly of a fibrous stroma with many large and bizarre cells and spindle cells mimicking leiomyosarcoma, many of which were pleomorphic and contained large bizarre hyperchromatic nuclei. Foci of atypical adult type cartilage and neoplastic osteoid formation were noted. In thetumortissue, membrane‐bound neurosecretory type cytoplasmic granules were demonstrated by electron microscopy and polypeptide hormone synthesis was demonstrated by immunohistochemistry. Furthermore, the patient suffered frequent attacks of sudden hypertension with hypercatecholamine

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01564.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

An autopsy case of high‐grade endometrial stromal sarcoma (ESS) is reported. Immunohistochemical study of the ESS was done in comparison with carcinosarcoma (CS), leiomyosarcoma (LMS) and normal endometrium in the uterus in order to trace the origin of ESS, which is a point of some controversy. Co expression of keratin and vimentin and/or desmin positivity in several elements of CS and LMS, and glandular or stromal tissues in normal endometrium made it difficult immunohistochemically to be certain of the origin of ES

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1990.tb01565.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY