摘要:

Masugi nephritis Induced in cholesterol‐fed rabbits is characterized by accumulation of foam cells in glomeruli which vary in number almost in parallel with the natural course of the disease. From electron microscopic appearances these foam cells are apparently derived from migrant blood monocytes and mesangial cells, but not from endothelial cells. Location of monocytic foam cells is extremely variable, though most often observed in the subendothelial space. In addition, monocytes undergo transformation into multinucleated giant cells including Touton type's. Lipid deposits filling the cytoplasm of foam cells seem to be in two forms, free cytoplasmic droplets and membrane‐bounded structures partly containing membranous debris or myelin figures. The latter accumulates preferentially in the cytocentrum and is considered to originate from lysosomes. It seems unlikely that glomerular lesions in Masugi nephritis are aggravated by foam cell accumulat

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1982.tb01395.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Histologic and ultrastructural features of nephroblastomas in Sprague‐Dawley rats induced by a single transplacental injection of ethylnitrosourea (ENU) on the 15‐16th day of gestation are described and their significance is discussed with respect to the histogenesis of the tumors. Many renal tumors were observed, in addition to predominant occurrence of tumors of the nervous system. Forty‐two of 142 offsprings survived over 100 days developed renal tumors, of which 37 rats had nephroblastomas, three rats had cortical adenomas, and one rat had a cystadenoma. The nephroblastomas were found in 35 rats unilaterally and in two rats bilaterally. Most of these tumors had various amounts of hyalinized stroma. In the kidneys with nephroblastomas, isolated, small foci of renal blastema were also occasionally noted. Electron microscopy revealed that nephroblastomas consisted of two types of cells, namely, epithelial and mesenchymal cells. The epithelial cells showed cellular differentiation of varying degree; they are freely lying in the stroma, forming well differentiated tubules or immature tubule‐like structures and solid cell nests. Most of the undifferentiated cells observed around the tubular structures and cell nests are demonstrated to have epithelial features. These observations seem to support the concept that nephroblastoma is originated from meta‐nephric

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1982.tb01396.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Five capillary hemangioblastomas exhibiting a range of histologic variations common to this tumor were studied using electron microscopy. The capillary hemangioblastomas were composed commonly of three cell lines — stromal cells, endothelial cells and pericytes. In addition, extramedullary hematopoietic foci were seen in the tumor tissue of one case. We regarded all these cellular elements as neoplastic components. The interconvertibility among them was not confirmed. The capillary hemangioblastomas were highly organized by those multiple cell lines. The histologic pattern of growth was arbitrarily subgrouped into stromal cell prominent type and capillary prominent type. Intermediate features between the two types were observed. These different types would depend on relative proportions and the degree of maturity of the constituting cell elements. From the histogenetic point of view, we considered capillary hemangioblastomas as being a hamartomatous tumo

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1982.tb01397.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Ultrastructural study of neuroblastoma group tumors including 7 neuroblastomas, 4 ganglioneuroblastomas, and 2 ganglioneuromas was performed by using both TEM and SEM. Tumor cells showed a wide variation comparable to the developmental stages of nerve cells and were classified into four types according to the neuritic process projections; namely apolar, monopolar, bipolar, and multipolar. Neuroblastoma was composed of small cells of apolar, monopolar, and bipolar types with few multipolar cells. Tumor cells of ganglio‐neuroblastoma and ganglioneuroma were multipolar type. SEM observation demonstrated characteristic varicosities in the elongated neuritic processes, and by TEM examination the dilated portions of these varicosities revealed disruption or disappearance of parallel runnings of the microtubules and microfilaments. TEM examination demonstrated presence of Schwann cells in ganglioneuroblastoma and ganglioneuroma, and satellite cells and perineurial cells in ganglioneuroma. The hitherto undescribed cells with caveolar structure which are thought to be a precursor of these stromal elements were disclosed in ganglioneuroblastoma. In order to evaluate the maturation and prognosis of tumors of this group, stromal differentiation seems to be equally important as ganglionic differentiation because both stromal elements and tumor cells have a common origin of neural cres

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1982.tb01398.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Histological and fluoromicroscopical studies were performed in order to obtain information about reversibility and associated tissue damage of an osmotic opening of the blood‐brain barrier. Three ml of 1.4 M mannitol sulution were perfused through the right carotid artery of a rat, and the opening of the blood‐brain barrier was examined by using Evans blue as a tracer. The barrier was opened for 60–120 minutes and then reestablished without gross neurological defect. Microscopically, however, edematous change and microinfarcts were often observed, which might be due to microembollsm of recrystallized mannitol. With filtration of the perfusate through a millipore filter, the blood‐brain barrier was reversibly opened without any tissue damages. This could be a useful therapeutic technique and an experimental model for neurotoxicology. ACTA PATHOL. JPN. 32: 427∼

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1982.tb01399.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Formation of hematogenous metastasis in the congenltally athymic nude mice (nu/nu) and their heterozygous ones (nu/+) was compared 2 weeks after an intravenous injection of xenogeneic tumor cells of rat ascites hepatoma cells (AH 130) with high thromboplastic activity. AH 130 cells formed large numbers of metastatic foci in the lungs of nu/nu recipients, while they developed into only temporary growth of metastatic foci with subsequent regression in nu/+ mice. Cellular reaction to arrested tumor cells in the pulmonary vessels was composed of large monouclear cells, and was extensive and prominent in nu+/ mice in comparison with nu/nu mice 3 days after the injection of the tumor cells. The results suggest that mononuclear cells reaction is closely related to suppression of growth of micrometastasis. This is considered as a model that highly immunogenic tumor cells attenuated and could be rejected in relation to immunological mechanisms. ACTA PATHOL. JPN. 32: 437∼443, 198

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1982.tb01400.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Changes of peroxidase (PO) activity in blood monocytes, untreated or stimulated peritoneal macrophages and subcutaneous exudate macrophages of rabbits were investigated with the glucose‐oxidase method in the culture experiments of these cells after their in vitro or in vivo adherence to the surface of foreign bodies. 1) PO activity was demonstrated in the nuclear envelope (NE) and rough endoplasmic reticulum (rER) in 75% of the untreated peritoneal macrophages. 2) Blood monocytes, most of the stimulated peritoneal macrophages, and subcutaneous exudate macrophages showed no PO positivity. 3) In the in vitro culture of blood monocytes and stimulated peritoneal macrophages, there appeared from 2 hours cells showing PO positivity in NE and rER. 4) Although blood monocytes, stimulated peritoneal macrophages, and subcutaneous exudate macrophages after their in vivo surface adherence showed no PO positivity, it appeared in these cells when further cultured in vitro. From the fact that such a positive change of PO activity in these cultured cells is not only a transient phenomenon but also in vitro cultural one, we cannot subscribe to the view that blood monocyte is the precursor of resident macrophages. ACTA PATHOL. JPN. 32: 445∼460, 1

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1982.tb01401.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Glycogen extracted from rat ascites hepatoma AH 13 cells was compared with that from adult rat liver, adult rat muscle, and fetal rat liver 3 days before delivery, by electron microscopic, biochemical and physicochemical techniques. The ascites hepatoma AH 13 glycogen revealed two major different types of glycogen macromolecules by sucrose discontinuous density gradient centrifugation. The glycogen of the light fraction was very similar to rat muscle glycogen macromolecules in its size and shape except that it exhibited a stronger Cotton effect on measurement of the optical rotatory dispersion and circular dichroism than the muscle glycogen. Furthermore, this light material different from normal muscle glycogen. The other glycogen which also included very small amounts of irregular particles of glycogen macromolecules was contained in the heavy fraction was very similar to the adult rat liver glycogen macromolecules which did not display the Cotton effect. Electron micrographs showing the ultrastructure of the glycogen particles extracted from the AH 13 cells by negative staining revealed a variety of size and shape, and they contained two types of glycogen macromolecules with the smaller muscle types and larger adult type of liver glycogen macromolecules. The glycogen macromolecules also formed varied irregular structures in conformation. ACTA PATHOL. JPN. 32: 461∼471, 198

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1982.tb01402.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

The morphogenesis of colonic adenomas in ICR mice treated with N,N′‐dimethylhydrazine dihydrochloride (DMH) was studied. Treatment of 17 mice with DMH (20 mg/kg/week) for 24 weeks induced 100% incidence of multiple adenomas in the mucosa of the most distal 7cm of the large intestine. These adenomas were composed of atypical epithelium of the absorptive cell type. Forty‐two single atypical gland lesions (single gland adenomas) were detected in the upper part of the mucosa by serial sectioning. These single gland adenomas consisted of atypical epithelium with a narrow lumen and showed endophytic growth to the lamina propria mucosae. Complete serial sections also revealed that single gland adenomas had no direct continuity of the surrounding crypts of Lieberkühn. Single gland adenomas developed into microscopic adenomas consisting of several atypical glands by branching of the small atypical glands. ACTA PATHOL. JPN. 32: 473∼4

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1982.tb01403.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

Commonly shared antigens between the renal tissue and lymphocytes, especially the thymocyte were studied In rats by immunofluorescent technique. To clarify the specificity of antisera, absorption analysis was carried out. The brain‐associated thymocyte antigen, presumably Thy 1 antigen, was detected by anti‐rat thymocyte serum (ATS) or anti‐rat brain serum (ABS) not only on the surface of thymocytes but also in renal glomeruli. The distribution or localization of the antigen in glomeruli was considerably different between acetone‐fixed and unfixed kidney sections. On the fixed section the antigen was shown exclusively in the glomerular mesangium. However, it appeared to be diffusely dispersed on glomerular tissue on the unfixed section. On the other hand, antisera against kidney‐related antigens, anti‐Fx1A, anti‐GBM, and anti‐CIG, did not react with the surface of thymocytes, or they were not absorbed with brain homogenate. ACTA PATHOL. JPN. 32

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1982.tb01404.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY