ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1951.tb00717.x

出版商:Blackwell Publishing Ltd    

年代:1951

数据来源: WILEY

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1951.tb00718.x

出版商:Blackwell Publishing Ltd    

年代:1951

数据来源: WILEY

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1951.tb00719.x

出版商:Blackwell Publishing Ltd    

年代:1951

数据来源: WILEY

摘要:

SUMMARY1. We succeeded in getting monocytes with ectromelia inclusion bodies from the peritoneal cavity of the intraabdominally infected mice.2. By this method, quantitative and qualitative development of inclusion bodies and cell reaction in ascites following infection were investigated. The inclusion bodies appear in the periphery zone of the cytoplasm having completely no relation with centrosphere of monocytes, and increase their volume gradually.3. In the supravital state the inclusion bodies stained positive with neutral red and fuchsin but negative with nile blue, brilliant cresyl blue and bordeaux BX.4. Elementary bodies within the inclusion bodies having blue brilliant points were found in the supravital preparations by the dark ground microscope.5. It was possible to take ultra‐violet photomicrographs of the infected monocytes in the supravital preparations. From data obtained by this method we observed the fine figures of elementary bodies in the ectromelia inclusion bodies corresponding to the blue points which had been found by the dark ground microscope.Absorption of ultra‐violet rays by inclusion bodies is much less than by the nuclei, and there is no difference between that of the inclusion, bodies and that of the protoplasm. The results obtained from the ultra‐violet microscopy coincide with the other results that the inclusion bodies give a negative Feulgenreaction in smear preparation. In this case the negative Feulgentest do not mean inevitably the denial of desoxyribonucleic nature of the virus nucleic

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1951.tb00720.x

出版商:Blackwell Publishing Ltd    

年代:1951

数据来源: WILEY

摘要:

SUMMARYExperiment I and experiment II suggested that streptomycin has an activity in the stimulation of phagocytosis in fixed cells. It is demonstrated that this activity continues for a week in mice injected with 125γ streptomycin. In experiment I (with lithiumcarmine injection) there occurs a very remarkable proliferation of fixed cells in the early period after streptomycin injection. In experiment II (with tubercle bacilli inoculation), it is a particularly noted that following streptomycin administration, degeneration of tissue cells in area adjacent to the necrotic center is very scarce and moderate proliferation of fixed cells with rapid reduction of wandering cell infiltration occurs.Necrotic fixed cells which contained a considerable number of tubercle bacilli formed caseous focus. These findings coincide with my studies in experimental tuberculosis treated with diluted Shwartzmancoli‐filtrate. These reveal a slight degree of degeneration with slight wandering cell infiltration compared with contro

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1951.tb00721.x

出版商:Blackwell Publishing Ltd    

年代:1951

数据来源: WILEY

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1951.tb00722.x

出版商:Blackwell Publishing Ltd    

年代:1951

数据来源: WILEY

摘要:

SUMMARYIn the lymphatic gland of well sensitized rabbits, there locate three groups of pyroninophile cells stained with the Unna‐Pappenheim'smethod. Namely, they are (1) germ centers, (2) sinus, especially the intermedially sinus and (3) lymphatic pulp near the medullary sinus.Among them, group (3) belongs to those plasma cells (Marschalko‐type), that arise through metamorphosis from adventitial cells of small blood vessels, especially of arterioles. Group (2) is constituted from the special cells which appear in such a condition in the efferent lymph, and which we nominated here as “lymphogonia”. Namely, they are large cells with deep basophilic protoplasm, and with large nucleus and giant nucleolus, and are often endowed with small clear zone perinuclearly. They show transition to lymphoblasts by Giemsastaining, but by supravital staining there appear a small grouping of fine neutral red vacuoles paranuclearly (corresponding to the clear zone by Giemsastaining), which resembles the neutral red vacuoles in plasma cells.Using phase control microscope, we reached the new knowledge that the discrimination of lymphocyte series from plasma cell series is possible with this method, referring to their natures of nucleus. Namely, the nucleolus of lymphatic series hang in the center of nucleus coloured orange, and large and polymorphous, and moreover, is bounded with thin, gray threads to the nuclear membrane. In plasma cells, this thick structure coloured orange, attached spottedly on the inner surface of nuclear membrane and from each of these spots thick processes rise toward the center, including nucleolus. In other words, our lymphogonias bear the nuclear character similar to lymphoblasts but not to that of transitional forms to plasma cells. This is the reason why we give a new nomenclature of “lymphogonias”.By the histological observation of sections, we confirmed the fact that these lymphogonias have transitions to reticulum cells in the lymphatic sinus, often in such a form with the processes like reticulum cells. The dissolution of such processes may easily move such lymphogonias into the efferent lymph.Such modus of metamorphosis of reticulum cells into lymphogonias is also seen in the germ centers. And we may easily understand such modus of proliferation as it recapitulate the embryonal lymphopoiesis. The reason why we did not controverse such a proliferation modus before, owes to our past understanding that the reticulum cells in lymph nodes were only thought as stroma cells of this organ. But from our new observation, the reticulum cells in lymphatic sinus refering to their nature of metamorphosis to lymphogonias, are cells which are determined toward the lymphatic series. We can not expect an analogous phenomenon concerning the cells of the bone marrow.As lymphogonias do not mature to plasma cells, we must deny the understanding of Fagraeusand others, which insist the maturation of reticulum cells to plasma cells with the transitional forms like that of lymphogonias. The metamorphosis of plasma cells from the adventitial cells of small vessels, especially of arterioles, takes place in Marschalko'sform from the beginning. The so often prevailed misunderstanding that lymphogonias mature to plasma cells, owes only to the observation of such organs that are endowed with lymphatic apparatus, for example, lymph nodes and spleens. We can avoid such a fault as to the observation of bone marrow or of connective tissues of subcutis or of omentum major, because there is no accompaniment of lymphogonias in the formation of p

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1951.tb00723.x

出版商:Blackwell Publishing Ltd    

年代:1951

数据来源: WILEY