摘要:

Experimental amyloidosis was induced in male albino rabbits and male mice (DD‐strain) with repeated administrations of either antigen‐antibody complexes or antigen and/or Freund's complete adjuvant for a long time. Spleen, lymphnodes, liver and kidneys were studied by light microscopy and fluorescent antibody technique, using anti‐rabbit IgG, anti‐mouse IgG, anti‐HGG or anti‐BSA as antisera.The homogenous substances of liver and spleen were induced in both animals in high frequency. The deposits in the lymphnodes and kidneys were also present in a minority of cases.The peripheral areas or a portion of these substances which were consistent physico‐chemically with para‐amyloid showed weakly positive reaction with fluorescent anti‐HGG or anti‐BSA in the same specimen in spite of the existence of large amounts of antibodies.The tissue toxicity of immune complex may play a significant role in the pathogenesis of amyloidosis in the consideration of the concurrent systemic vascular changes and immune dissociation which suggest the possibility of disturbed immune mechanisms as a common causative background. In the present study using insoluble complexes, some questions still remain to be solved on the pathogen

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1972.tb00737.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

In order to obtain an isolated cell culture of reticuloendothelial cells the mouse liver has been digested by Pronase and cultured up to one year. Propagated cells in vitro have shown four different cell types; remarkable phagocytically active cells‐type I; giant cells‐type II; less phagocytically active cells‐type III; and epithelial cells‐type IV. These cells of types I, II and III have been considered to be of sinusoidal endothelial origin and the diminution of phagocytic activity in propagated cells after long‐term cultivation in vitro has been regarded to be the result of adaptation to the in vitro environment.By means of the cultivation of amyloid liver of mice, synthesis of amyloid by sinusoidal endothelial cells has been observed and a close relationship between reticuloendothelial cells and amyloid has been proved

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1972.tb00738.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Rabbits were treated with the prolonged administration of γG fraction of goat anti‐rabbit lymphocyte serum (ALγG) over 180 days. The effect of ALγG on various kinds of immune responses was examined at different stages of the experiment.The antibody against ALγG appeared at an early stage. Especially the formation of γM antibody was rather enhanced as compared to the control animals which received normal goat γG (NγG). The antibody titer increased, and then maintained its level through the course of experiment. However, a slight decline of the titer was noted in the last stage. The Immune responses against sheep red blood cells, bovine serum albumin and skin homograft were greatly suppressed by the short‐term treatment of ALγG. On the other hand, no suppression of the immune responses was demonstrated in the group treated with ALγG for 180 days as compared with the control group.The results indicate that ALγG used is effective to suppress the immune responses in the short‐term treatment, but it is probably neutralized by the antibody and causes no further immunosuppression. In addition, it has also been confirmed that an exhaustion of the lymphoid tissues accompanying occasional amyloidosis might be induced by the long‐term admin

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1972.tb00739.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Tuberculin reaction in guinea pig mesentery has been studied as a model of delayed type hypersensitivity. The results obtained through these series of experiments are; 1) even in the delayed type hypersensitivity, the initial phase of the reaction consists of microclrculatory disturbances similar to those of the immediate type reaction, and 2) the capacity of lymphatic drainage after plasma exudation is the essential factor for the formation and absorption of granulomas which are located in rather far distant areas from the vascular tree.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1972.tb00740.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The process of degranulation and regranulation of pancreatic islet cells was followed in the male rats given one intramuscular or intraperitoneal injection of alloxan (200 mg/Kg). The serum glucose showed a typical triphasic curve, reaching permanent hyperglycemia 24 hours after injection. The insulin levels of both serum and pancreas have been subnormal for the first 24 hours and then marked decrease of insulin levels was observed on the third day thereafter. In accord with subnormal pancreatic insulin levels, B granules have been present in the necrotic cells up to the first 24 hours, then complete disappearance was observed on the third day. Histochemistry of zinc was parallel to the preservation of B cells. Reactive zinc decreased from the center of the islets In 2–4 hours after alloxan, and after 24 hours zinc reaction was positive only around the periphery of the islets. Regranulated islet cells were observed from 7 days through 28 days in the presence of permanent diabetes with decreased population of B cell

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1972.tb00741.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The ultrastructural study of pancreatic islets from the alloxanized rats was performed along with histochemical studies. During the initial stages of hyperglycemia, B cell degranulation was progressive, while A cells were left intact. The presence of remaining B granules was confirmed in the disintegrated cell debris and also in the macrophages up to 24 hours. The islets from the third day after alloxan showed only a few degranulating B cells and the majority were small poorly differentiated agranular cells and A cells. Major components of small islets from 7 days through 28 days were A and D cells, while some large islets were composed of a mixture of well regranulated A cells and sick B cells.The significance of agranular cells as the possible origin of the differentiated A and B cells was suggeste.

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1972.tb00742.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

We originally attempted to induce a gastric carcinoma in 327 female rats by the oral administration of 2,7‐dlacetylaminofluorene. Gastric carcinoma, however, did not appear, while leukemia was found In 31 cases (9.5%). Chronic myelogenous leukemia was encountered in 29 cases (8.9%). This leukemia in rats morphologically resembles chronic myelogenous leukemia in ma

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1972.tb00743.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

A study was made on the early effects of localized X‐irradlation (a single dose of 2000 rads) on the ultrastructure of the gastric mucosa of mice by observing the changes from 15 minutes following irradiation to the 7th day when ulcer developed. The six types of gastric epithelial cells all demonstrated differences in the initial cytoarchitectural changes of radiation injury and in radiation sensitivity. The cell sites or cell organelles which exhibited the earliest radiation lesion were the Golgi body and endoplasmic reticulum in the mucoid cells, cell membrane in the parietal cells, endoplasmic reticulum in the chief cells, and secretory granules in the endocrine cells. Cells demonstrating the highest sensitivity to X‐ray were surface and pit cells, followed by pyloric gland cells or mucous neck cells, parietal cells, and chief cells with endocrine cells showing the highest radiation resistance. These observations suggest a close relationship of ultrastructural alteration of radiation effects to not only the presence or absence of cell division, the degree of cells differentiation, and cell function but also to the development of cell organelle.The pyloric mucosa was the gastric region which demonstrated the greatest radiation cell damage, and only in the mucoid cells could be observed radiation induced mitotic inhibition or delay and giant cell formation due to the injury of cell divis

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1972.tb00744.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

Four additional chorionic tumors have been obtained by the same method as previously reported1 using 4‐NQO and 7,12‐DMBA as carcinogens. It was observed that high dosage of DMBA was quite effective in inducing chorionic tumors in rats. Gross and histological appearance of the tumor resembled that of normal placenta in rats. Part of the tumor has been transplanted and is now under observat

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1972.tb00745.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY

摘要:

The effect of peroral application of pyrldinolcarbamate (30 mg/kg/day) was studied in rats which were kept either on a standard laboratory diet, or on a diet containing a large amount of fat (40%) and cholesterol (5%). PDC significantly increased the lactate dehydrogenase activity in the aortae of rats kept on a standard diet, and the malate dehydrogenase activity in the aortae of rats kept on high‐fat diet.In experimentsin vitro, PDC increased the activity of the aortic lactate and malate dehydrogenase. This effect was most pronounced at higher concentrations of PDC in the incubation medium (3000μg/ml). In the rat myocardium, activity of the enzymes studies was not noticeable influenced by PDC administration. Under the given experimental conditions, pyrldinolcarbamate increased the activity of enzymes participating in the citrate cycle and glycolysis. In view of the present knowledge of the role of the vascular wall metabolism in atherogenesls, this effect could contribute to greater resistance of the vessel wall against the development of atherosclerotic chang

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1972.tb00746.x

出版商:Blackwell Publishing Ltd    

年代:1972

数据来源: WILEY