摘要:

Oral administration of N‐methyl‐N′‐nitro‐N‐nitrosoguanidine induced gastrointestinal tumors in 15 out of 19 rats: six adenomas, seven adenocarcinomas, one fibrosarcoma and one malignant schwannoma that was homotransplantable. Both the original and transplantable tumor exhibited characteristic morphological features and immunoreactivity identical to that of a human malignant schwannoma: positive reaction for S‐100 protein, neuron specific enolase, glial fibrillary acidic protein, myelin basic protein and vimentin. In addition, α‐smooth muscle actin was expressed in both tumors. Cultured tumor cells derived from the transplantable tumor at passage 3 produced 18 clones which showed anchorage independent growth in soft agar. From these clones, two cell lines showing characteristic immunoreactivity, designated as RMS‐1 and 2, were established. In general, the immunoreactivities of the two cell lines were similar to those of the original tumor; however, the RMS‐1 cell line demonstrated positive immunoreaction for neurofilaments and RMS‐2 was negative for α‐smooth muscle actin. Subcutaneous injection of cultured cells from both cell lines into athymic BALB/c nude mice induced tumors identical to the original tumor. In the present study, transplantable malignant schwannoma was established in the rat and two phenotypes were isolated and

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb03229.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Three cases of Lewy body disease were investigated in order to compare the morphological and immunohistochemical characteristics of the neuronal inclusions in the cerebral cortex (CC) and brain‐stem (BS). Ultrastructurally, the CC contained intermediate‐sized filaments with variable amounts of granular material and other organelles, whereas the BS consisted of an electron‐dense core and an outer area with radially oriented filaments. The cerebral cortex was immuno‐reactive with antibodies against tyrosine hydroxylase (TH) and tau protein, and differed from BS. In addition, although the CC were antigenically similar to BS in their neurofilament (70, 160 and 200 kDa) and ubiquitin contents, the localization of neurofilament immunoreactivity differed between them, being confined positively to the core of CC, but to the periphery of the BS. Although Lewy bodies (LB) in idiopathic Parkinson's disease are morphologically similar to BS, they have been reported to differ in their immunoreactivity with antibodies against tau. It has been reported that CC differ from LB with regard to immunoreactivity with antibodies against TH and tropomyosin. It is inferred that these inclusions (CC, BS and LB) differ in morpho

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb03230.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Twenty‐one renal biopsy specimens obtained from 10 patients with dense deposit disease (DDD) were investigated using light microscopy, electron microscopy and immunohistochemistry. The patients included four females and six males aged 6 to 35 years (mean 16.1 years). A morphological diagnosis of DDD was made following the ultrastructural detection of continuous intramembranous dense deposits (CIMDD) in some capillary loops of at least one of the series of the repeated biopsies from each patient. With light microscopy, six patients showed membranoproliferative glomerulonephritis (MPGN). The other four patients showed diffuse proliferative glomerulonephritis (DPGN) with acute lesions showing intraglomerular neutrophilic infiltration, hump formation and endothelial swelling in three and minor glomerular abnormalities in one. Follow‐up biopsies were obtained in six patients. Two patients progressed from DPGN to MPGN within 7 months, whereas three patients with MPGN showed morphologic improvement that featured increased capillary patency and regional disappearance of dense deposits along with the reduction of proteinuria. Dense deposit disease did not always feature typical amorphous and osmiophilic CIMDD spreading across the whole width of the lamina densa. This classical ultrastructural manifestation was mainly found in the patients with histologic non‐MPGN and a linear peripheral pattern of complement component (C3) deposition. The MPGN patients with a granular peripheral pattern of C3 deposition also had CIMDD, but also additionally featured less dense subepithelial deposits superimposed on the CIMDD to produce an appearance simulating membranous transformation. Humplike epimembranous massive dense deposits were also identified in connection with the deposition of immunoglobulin G (IgG), suggesting that immune complex deposition at the glomerular basement membrane occurs in some cases of DDD. Immunoglobulin M (IgM) or complement component 1q (C1q) deposition was often associated with intraglomerular neutrophilic infiltration and endothelial swelling as well as with ultrastructural subendothelial edema. Continuous dense deposits were found not only in the lamina densa but also just beneath the subendothelium in four patients. Thus, the present investigation demonstrated the morphologic variety of DDD in a correlative study of light microscopy, electron microscopy and immunohlstochem

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb03231.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

A new human cell line, LC‐2/ad was established from pleural effusion of pulmonary adenocarcinoma of a 51 year old Japanese female. The LC‐2/ad cells exhibit an epithelial appearance and a tendency to form small domes as observed with phase‐contrast microscopy. The modal chromosome number was 53–56. Plating efficiency and doubling time were 6.8% and 58 h, respectively (32th passage). Immunocytochemlcally, the cells were strongly positive for CEA and cytokeratins including cytokeratin no. 18 which is present in simple epithella. Ultrastructurally, the cultured cells were characterized by well‐formed junctional complexes and microvilli. Subcutaneous injection of 5 × 106cells into a nude mouse resulted in tumor formation classified histologically as a moderately differentiated adenocarcinoma. This cell line produced at least two functionally active trypsin inhibitors together with several proteinasesin vitro.The main inhibitor was purified partially from the serum‐free conditioned medium and confirmed immunologically as human α1‐antitrypsin (AAT). Immunohistochemically, the xenografted tumor was also positive for AAT. The cell line LC‐2/ad is useful for the study of tumor‐derived serine proteinase inhibitors

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb03232.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Epstein‐Barr virus (EBV) involvement in gastric carcinoma has been demonstrated by the presence of EBV genomes and EBV‐encoded small RNA (EBER) in the carcinoma cells, monoclonal proliferation of EBV‐infected carcinoma cells and elevated antibody titers. The present study was conducted to investigate the prevalence of EBV involvement among gastric carcinomas observed in nine Japanese cities with varying gastric cancer rates.In situhybridization of EBER‐1 was applied to paraffin sections from 1848 carcinomas observed in 1795 cases and EBV involvement was detected based on uniform hybridization in carcinoma cells. Epstein‐Barr virus was detected in 6.6% of lesions and 6.7% of cases. The rate of EBV involvement did not vary significantly for each city and there was no correlation with underlying gastric cancer mortality rates. Thus, geographic variation of gastric cancer rates within Japan cannot be explained in terms of EBV involvement. Epstein‐Barr virus‐related gastric carcinoma is one of the most common EBV‐related tumors in Japan. The involvement of EBV was significantly more frequent among males than among females, mainly for cancers occurring in the upper and middle part of the stomach, and exhibited more variation by cell type among males. These observations suggest that other factors yet to be discovered may modulate the causal role of EBV in gastric

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb03233.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Basic fibroblast growth factor (bFGF) was identified in the papillary carcinoma of the human thyroid. Immunohistochemically, it was found that the reactivity for bFGF was localized in the cytoplasm of the neoplastic cells of the five papillary carcinomas. However the extract of the papillary carcinomas contained the mitogenic activity for endothelial cells. This bioactive molecule was determined as bFGF by using the heparin‐Sepharose affinity chromatography and western blot analysis. The bFGF derived from human thyroid papillary carcinoma and the recombinant human bFGF stimulated the bromodeoxyuridine incorporation by the cultured human thyroid papillary carcinoma cells. These cells also showed positive staining for thyroglobulin and cytokeratin. These results indicate that bFGF, probably produced by the neoplastic cells, plays an important role in the development of papillary carcinoma of the thyroid with stimulation of angiogenesis as well as proliferation of the parenchymal cell

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb03234.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Tumor cell kinetics and DNA contents were investigated byin vitrolabeling with bromodeoxyuridine (BrdU) and flow cytometry using paraffin‐embedded specimens in both superficially (Sup) and deeply (Deep) infiltrating sites of 28 colorectal adenocarcinomas. Eleven were well differentiated and 17 were moderately differentiated adenocarcinomas. In moderately differentiated adenocarcinoma, histologic low grade types of Deep were frequently associated with lymph node metastasis, but the BrdU labeling indices (LI) or tumor DNA ploidy pattern did not correlate with nodal status. Among the seven aneuploid tumors of moderately differentiated adenocarcinoma, the BrdU LI and histology of Deep tended to correlate with nodal status. These findings suggest that the histology of Deep should have a greater significance than tumor cell kinetics or DNA contents. They also suggest that the tumor DNA ploidy pattern may be heterogeneous and divided into subgroups according to its cell kinetics or histology of Deep in relation to lymph node metastasi

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb03235.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

A rare case of solitary Ileal duplication cyst accompanied by heterotopic pancreas in the terminal ileum is presented. An 8 year old boy with an ovoid shaped, elastic soft and pendant‐growing ileal duplication cyst associated with aberrant pancreatic tissue but not communicating with the lumen of the ileum, underwent an adhesiotomy for a small intestinal adhesion that caused bowel obstruction. The obstructive state of the intestine seemed to be caused by an inflammation of the duplication cyst which was 4.5 × 2.7 × 2.5 cm and oval in shape. Excision of the cyst and the heterotopic pancreas was curative. The histological findings were identical to heterotopic pancreas accompanied by ileal duplication without any clinical features of heterotopic pancreas. A review of the literature is presented along with the authors' experience in a case of ileal duplication cyst associated with heterotopic pancreas located in the il

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb03236.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

A recurring case of Castleman's disease containing a fibrohistiocytic nodular lesion is presented. The recurred tumor was found in the mediastinum of a 40 year old Japanese female who had undergone the extirpation of a mediastinal tumor 18 years previously and had been suffering from rheumatoid arthritis 5 years after the initial removal. Microscopically, the present tumor and the initial removed tumor fulfilled the morphologic criteria for the hyaline‐vascular type of Castleman's disease. A well‐demarcated nodular lesion (1.5 cm in diameter) was located in the present tumor. It consisted of interlacing oval to short spindle‐shaped cells positive for factor XIIIa and vimentin, merging with lymphocytes, plasma cells, macrophages and capillaries lined by swollen endothelial cells. Mitotic figures were not noted. Foci of hemorrhage and necrosis were also not detected. The stenosis or occlusion of the large vessels due to intimal fibrosis and medial hypertrophy was observed around the nodular l

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb03237.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Three cases of lung carcinomas with unusual histologic appearances that have received little or no comment in the literature are presented. They were initially confused with malignant lymphoma because of a diffuse proliferation of relatively monotonous cells simulating large‐cell immunoblastic lymphoma. In each case, the possibility of malignant lymphoma was excluded with confidence after the immuno‐histochemical study (leucocyte common antigen negative and cytokeratins positive), although with conventional microscopy several foci of cohesive groups of tumor cells were observed. The tumors were ranked at the clinical stage II or III when they were initially discovered, but all patients died of disease within 1 year. The present three tumors show an aggressive behavior and could be classified into a peculiar variant of ‘large cell’ carcinoma. It is necessary for surgical pathologists to have an idea of these variants of lung carcinoma in order to avoid erroneous di

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1993.tb03238.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY