摘要:

Histological localization of mammary tumor virus (MTV/) antigen was investigated using a variety of organs high (DD/Tbr, SHN, SLN, GR) and low (BALB/c) mammary cancer mice strains and immuno‐peroxidase staining with MTV antigen. Except for BALB/c strain mice, the mammary gland and mammary tumors were generally positive. Accessory male genital organs including the prostate, seminal vesicle, and coagulating gland also demonstrated a positive reaction, but the testis and female genital organs including uterus and ovaries did not. MTV antigen was also revealed in the serous acini of the salivary gland in both sexes. The site of positive reaction in the accessory male sex organs and salivary gland was located in the apical portion of the secretory epithelial cells and their secretory substance. Localization and intensity of antigenic expression of MTV detected histologically were comparable to results obtained by immunodiffusion test and radioimmunoassay. These evidences support the idea that MTV is transmitted horizontally via seminal fluid or saliv

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01037.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The participation of glomerular antigen presenting (la positive) cells in the development of glomerulonephritis was analyzed. Antigen‐presentation by kidney cells was evident from the assay of ovalbumin‐speciflc T cell proliferation responses by these cells. Experimental serum sickness nephritis was induced in preimmunized rats by the intraperitoneal sensitization of oval‐bumin, and the participation of antigen presenting cells (APCs) in the peritoneal cavity and glomeruli was studied. APCs in the peritoneal cavity progressively increased in number following the antigen stimulation, while those in glomeruli were shown to increase in correlation with the development of glomerular hypercellularity. These results imply that the role of APCs in the sensitized area is superior in this model of immune complex nep

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01038.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

A trypsin‐like protease(s) was found in the extracts from tuberculin reaction sites in the guinea pig skin. Extractable enzyme activities were chronologically paralleled with the gross appearance of the inflammation, and the maximum activity from 24‐hour old inflamed sites was about 12 times stronger than that from control skin, suggesting a potential role in the pathogenesis of the delayed hypersensitivity reaction (DHR). The enzyme suitably hydrolyzed t‐butyloxycarbonyl‐phenylalanyl‐seryl‐arginine 4‐methylcoumaryl‐7‐amide, and was partially purified by gel filtration. It showed an apparent molecular weight of 700,000 with optimal pH of 9.0–9.5. The activity was inhibited by various serine protease inhibitors but not by thiol or metal protease inhibitors. It was relatively heat‐stable. These findings suggest that the protease is similar to a trypsin‐like protease from DHR to bovine γ‐globulin. Intradermal injection of the enzyme into normal guinea pigs induced a mixed neutrophil‐mononuclear infiltrate. The reaction was reduced in intensity with the dilsopropylfluorophosphate‐inhibited enzyme preparation. The enzyme did not induce any increased vascular permeability. These findings suggest that the protease may be present generally in the DHR sites and probably participates i

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01039.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The role of Lactobacillus arabinosus in the malignant transformation of tumors of the large intestine was investigated in mice. Methylazoxymethanol (MAM) acetate, at a weekly dose of 0.2mg/10g body weight, was given to germfree mice and to mice monocontaminated with eitherL. arabinosusorEscherichia coli.At sacrifice, the activity of non‐specific esterase, β‐glucuronidase, and alcohol dehydrogenase within the liver and intestine was examined biochemically and histochemically. Non‐specific esterase activity in the liver and large intestine was significantly higher inL. arabinosusmice than in the other 2 groups. Also, β‐glucuronidase activity in the large intestine and alcohol dehydrogenase activity in the liver were significantly greater inL. arabinosusmice than in the other groups. Esterase was localized in the mitochondria and absorptive granules within the mucosal epithelium of the large intestine. An apparent increase in the number of certain organelles was observed in theL. arabinosusmice, compared with the other groups. These results suggest thatL. arabinosusplays an important role in MAM acetate tumorigenesis and malignant transformation. AC

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01040.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The association between hepatocellular carcinoma (HCC) and chronic hepatic schistosomiasis (CS) was studied by reviewing, 4,886 autopsies in adults during the past 20 years. In 229 cases of CS, 59 (25.7%) also had HCC. Among cases without CS, 399 (8.5%) had HCC. The incidence of HCC in patients with CS was significantly higher than that of other autopsy cases (p<0.01). Serum HBsAg was positive in 25.7% of 35 HCC cases with CS examined for hepatitis B virus (HBV) markers and anti HBs was positive in 10 of the 12 HBsAg‐negative cases associated with CS, and in 62.1% of the other HBsAg‐negative cases examined. Thus, most of HCC cases, including those associated with CS, probably had HBV infection at one time. Morphological examination revealed varying degrees of non‐schistosomal hepatic changes, including macronodular or mixed macro‐and micronodular cirrhosis, superimposed on schistosomal fibrosis in about two‐thirds of the cases of HCC associated with CS. Although conclusive evidence whether or not schistosomal infection had a direct role in hepatocarcinogenesls could not be obtained, it was predicted that the additional non‐schistosomal factors, particularly HBV infection, might play a syner

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01041.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

The bronchial epithelium in stepwise transverse sections was examined histologically in 66 male autopsy cases, composed of the groups of 19 mustard gas (MG) ex‐workers with lung cancer, 17 MG ex‐workers with non‐lung cancer, 10 non‐MG lung cancer cases, and 20 non‐MG non‐lung cancer cases. Foci of moderate or severe atypical cellular lesion or dysplasia, or of carcinoma in situ (CIS) in total slides of each group, were counted as 146 in 3,485, 72 in 2, 226, 70 in 3,797, and 18 in 4,611, respectively. The relative frequency of moderate or severe dysplasia and CIS in MG exposed non‐lung cancer cases resembled that found in lung cancer cases of both MG and non‐MG exposed. Seven CIS lesions were detected from among all MG‐exposed cases and one CIS was found in a non‐MG lung cancer case. Six out of eight CIS examples were adjoined by dysplasia. A multi‐variate analysis revealed a significant correlation between the incidence of atypical lesions and MG exposure, though the incidence of atypical lesions was also influenced significantly by age, smoking, and chronic bronchitis. The incidence of atypical lesions was significantly higher in cases of squamous cell lung cancer than those of other histological types, particula

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01042.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Adrenocortical adenomas with Cushing's syndrome (Cushing's adenoma) consisted of two different features, which intermingled in various proportions and were related with each other. The first feature showed long‐columnar or trabecular arrangement and a vacuolar appearance which correspond to lipid droplets of clear‐type cells. In the second feature, the sinusoid was well developed and meandering, and the perisinusoidal space was prominent and contained collagen fibrils, bleb‐like structures, and granules. The collagen fibrils were entangled with the parenchymal cells. The sinusoid was lined by a sinusoidal wall with fenestrations or pores. Bleb‐like structures and granules traversed them. Two different features were speculated to be consistent with areas of clear‐type cells and compact‐type cells, respectively, observed light and transmission electron m

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01043.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Early hypoxic‐ischemic brain lesions were examined regarding 26 autopsy cases which had severe asphyxia at birth and died within the first week. All cases were divided into three groups according to the birth weight: group A of less than 1,000 grams, group B of 1,001–2,500 grams, and group C of more than 2,501 grams. Neocortical and deep gray matter revealed pyknotic and karyorrhectic neuronal changes; however, in group A, these changes tended to be obscure. The hippocampus was the predictive site of the neuronal changes such as pyknotic neurons in Sommer's sector and karyorrhectic neurons in subiculum. In 12 cases, pontosubicular‐type necrosis was found. White matter lesions were relatively characteristic and there was an early appearance of pathological astrocytes such as gemistocytic, Alzheimer‐type 2 and stellate astrocytes, periventricular leukomalacia with or without hemorrhage. The brain stem and cerebellar lesions were also found occasionally revealing neuronal or glial changes. We applied the immunoperoxidase method using antisera to glial fibrillary acidic protein (GFAP) and S‐100 protein for determination of pathological astrocytes. GFAP was a useful marker for pathological astrocytes in the subpial region and in the white matter. S‐100 protein was present in Bergmann's glia and satellite glia as well as pathological

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01044.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

An extremely rare abnormality, a variant of omphalomesenteric duct, was observed in the umbilical cord 1 cm from the entry point into the abdominal wall, which itself was as an isolated mass of well‐formed intestinal tissue free of structural defects. This intestinal tissue seems to be the remnant of an intestinal loop from caudal limb of midgut following physiological herniation during the third month of gestatio

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01045.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

This is a case report of malignant endometrioid adenofibroma of the ovary with serous cystadenoma in a 42‐year‐old female. It was classified as FIGO serous IA (2) ovarian tumors. Serous epithelium transformation into endometrioid glandular epithelium was disclosed. The patient is now in good condition and followed‐up periodi

ISSN:1320-5463    

DOI:10.1111/j.1440-1827.1986.tb01046.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY