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1. |
Differences in some erythroid regulatory parameters between two inbred mouse strains |
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The International Journal of Cell Cloning,
Volume 1,
Issue 5,
1983,
Page 316-323
C. D. R. Dunn,
L. Gibson,
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摘要:
AbstractNormal erythropoiesis in inbred CBA/Tr × CBA/Tr mice is shown to be associated with higher rates of red blood cell production and destruction than in age‐matched, but significantly larger, C57B16/Tr × C57B16/Tr mice. As expected from these observations, serum Ep titers were significantly higher in the CBA/Tr mice than in the C57B16/Tr mice. These differences, which appear to result from variations in the operating point for erythropoiesis, can explain the different in vitro erythropoietin sensitivities of hematopoietic tissue from these two mouse stra
ISSN:0737-1454
DOI:10.1002/stem.5530010501
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1983
数据来源: WILEY
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2. |
Stem cells and immunological parameters in mice during the latency period and after the development of chemically induced leukemia |
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The International Journal of Cell Cloning,
Volume 1,
Issue 5,
1983,
Page 324-342
Hans Joachim Seidel,
Susanne Pfeiffer,
Ludwika Kreja,
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摘要:
AbstractT‐cell leukemias have been induced in adult BDF1mice by 12 or 15 weeks of exposure to butylnitrosourea (BNU) in the drinking water. This led to a depression of CFU‐S numbers and reduced T‐ and B‐cell responses to mitogens. These parameters were then studied during the BNU‐free preleukemic latency period in individual mice. At the same time, leukemic cells were traced in the thymus, the spleen, and the bone marrow by transplantation. In mice without leukemia and mice with leukemic cells in only one organ, there was a general tendency to normal CFU‐S numbers and T‐ and B‐cell responses with time after BNU, although control levels were reached in only a few of the mice. The reaction of mixed lymphocyte cultures (MLC) remained low during the latency period. In the thymus an imbalance of the Con A, PHA, and MLC responses was observed. Out of 25 mice with induced leukemia, 8 had leukemic cells in the thymus only and 2 in the marrow only. In mice with leukemic cells in all 3 he‐mopoietic organs and an enlargement of the spleen, a shift of CFU‐S from the marrow to the
ISSN:0737-1454
DOI:10.1002/stem.5530010502
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1983
数据来源: WILEY
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3. |
Incidence and characteristics of colony‐forming units fibroblasts (CFU‐F) in the bone marrow of weanling mice treated with cytosine arabinoside and phenylhydrazine: Correlation with CFU‐S, progenitors of diffusion‐chamber colonies (CFU‐D), and CFU‐C |
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The International Journal of Cell Cloning,
Volume 1,
Issue 5,
1983,
Page 343-359
Zina Ben‐Ishay,
Amiram Borenstein,
Sara Sharon,
Gregor Prindull,
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摘要:
AbstractA study of murine adherent marrow cells (AMC) under conditions of high and low concentrations of hematopoietic stem cells and progenitors was carried out. In one group of weanling mice, decreased marrow cellularity and increased concentrations of CFU‐S, CFU‐D, and CFU‐C were observed two days after administration of two consecutive intraperitoneal (i.p.) cytosine arabinoside (Ara‐C) injections (2 × 200 mg/kg, at 6 h interval). Fibroblast colony‐forming units (CFU‐F) of this marrow were studied. In a second group of mice, which were given three i.p. phenylhydrazine (PHZ) injections (3 × 60 mg/kg on days 0, 1, and 3), CFU‐S and CFU‐C levels were unchanged or lowered 6 days after the start of PHZ administration. In these animals, however, the number of CFU‐D was four times higher than in controls. The study of CFU‐F in experiments of groups 1 and 2 indicated a high concentration of these progenitors in group 1 and lower concentration in group 2. Furthermore, fibroblastoid colonies produced in vitro by CFU‐F of Ara‐C‐treated marrow were significantly larger than colonies from control marrow, and they markedly inhibited G/M colonies in split‐phase agar cultures. By contrast, fibroblastoid colonies produced by PHZ‐treated marrow were of regular size and did not inhibit G/M
ISSN:0737-1454
DOI:10.1002/stem.5530010503
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1983
数据来源: WILEY
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4. |
Surface antigens of murine hemopoietic stem cells. VIII. antisera define lineage antigens held in common between granulocyte‐macrophage cells and between lymphoid cells |
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The International Journal of Cell Cloning,
Volume 1,
Issue 5,
1983,
Page 360-376
M. V. Berridge,
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摘要:
AbstractThe cell‐lineage model of hemopoietic cell differentiation has been further investigated by detailed absorption analysis of the anti‐stem cell activity in rabbit antisera against mouse hemopoietic cells. Of seven differentiated hemopoietic cells tested, platelets alone absorbed the anti‐stem cell activity in anti‐platelet serum. Thymocytes and B‐lymphocytes absorbed all of the anti‐stem cell activity in antithymocyte serum whereas other nonlymphocytic cells showed only partial absorbing ability. Macrophages and granulocytic cells absorbed most of the anti‐stem cell activity in antimacrophage serum and antineutrophil serum whereas other cell types showed little or no absorbing capacity. Antisera against a cloned mast cell precursor line showed partial cell lineage activity whereas antisera against eosinophils and B‐lymphocytes showed no evidence of cell lineage activity. A detailed model of cell lineage antigens on hemopoietic cel
ISSN:0737-1454
DOI:10.1002/stem.5530010504
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1983
数据来源: WILEY
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5. |
Clonal growth of human megakaryocytic progenitor cells in a micro‐agar culture system: Simultaneous proliferation of megakaryocytic, granulocytic, and erythroid progenitor cells (CFU‐M, CFU‐C, BFU‐E) and T‐Lymphocytic Colonies (CFU‐TL) |
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The International Journal of Cell Cloning,
Volume 1,
Issue 5,
1983,
Page 377-388
D. Geissler,
G. Konwalinka,
C. Peschel,
J. Boyd,
R. Odavic,
H. Braunsteiner,
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摘要:
AbstractA simple and reproducible micro‐agar culture technique for cloning human CFU‐M is described. Human bone marrow mononuclear cells were suspended in agar and incubated for 12 days. Stimulation was provided by the direct addition of phytohemagglutinin‐P (PHA‐P), erythropoietin (Epo) and 2‐mercap‐toethanol (2‐ME) to the liquid overlayer. A shift from BFU‐E and CFU‐C proliferation to CFU‐M and CFU‐TL was observed with increasing PHA concentrations. Under optimal conditions (PHA 50 μg, Epo 1.2 IU, 2‐ME 2 × 10‐4M, 1% purified BSA, 0.04% human transferrin, saturated with Fe Cl3) a linear relationship between colonies formed and plated cell number was observed. For the routine morphological analysis, the whole agar layers were stained using the Pappenheim method. For further characterization of CFU‐M, cytochemical stainings and immunofluorescence tests with rabbit‐antihuman factor VIII‐related antigen were p
ISSN:0737-1454
DOI:10.1002/stem.5530010505
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1983
数据来源: WILEY
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6. |
Identification and distribution of megakaryocyte colonies in murine spleen |
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The International Journal of Cell Cloning,
Volume 1,
Issue 5,
1983,
Page 389-400
Chandra Choudhury,
Pamela Allman,
Eugene Arnold,
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摘要:
AbstractThis study is the first report on the utilization of specific cell function to identify splenic megakaryocytic colonies. Stem cell differentiation into megakaryocytes was studied by injecting each irradiated murine syngeneic recipient with 1 × 106spleen cells. Morphological identification of erythroid, gran‐ulocytic, megakaryocytic, and mixed and undifferentiated colonies was done by staining consecutive cryostat sections with hemotoxylin and eosin, benzidine, myeloperoxidase, and acetylcholinesterase. The variation in the distribution of hemopoietic colonies within the spleen was reflected in the different ratio values derived for erythroid, granulocytic, and megakaryocytic colonies at varying depth within the spleen. An increase by 50% of megakaryocyte colonies was seen within the splenic pulp in the midzone region, compared with the surface. This suggests a localized microenvironment conducive for megakaryocytopoiesis. The data emphasizes the importance of identifying colonies of all cell types in histological sections of the spleen and evaluating spleen sections at least at two levels, one adjacent to the surface and the other in the midzone ar
ISSN:0737-1454
DOI:10.1002/stem.5530010506
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1983
数据来源: WILEY
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7. |
Myelopoiesis of human bone marrow cells in a micro‐agar culture system: Comparison of two sources of colony stimulating activity (CSA) |
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The International Journal of Cell Cloning,
Volume 1,
Issue 5,
1983,
Page 401-411
G. Konwalinka,
C. Peschel,
D. Geissler,
J. Boyd,
B. Tomaschek,
R. Odavic,
H. Braunsteiner,
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摘要:
AbstractHuman bone marrow cells were grown in a micro‐agar culture system in the presence of human placenta (HPCM) and giant cell tumor conditioned media (GCT). The effects of HPCM and GCT conditioned media on linearity, growth dynamics, and morphological composition of colonies were studied after 7 and 14 days of incubation. Under the described conditions the dose‐response curves for HPCM and GCT were different: on day 7 maximal stimulation was obtained with 2.5% HPCM and 20% GCT; on day 14 a maximal response was reached with 1.25% HPCM and 5% GCT. Both stimuli produced maximal growth after 7 days of incubation, followed by a rapid decrease in the number of formed colonies up to day 14. The morphological study of aggregates showed that after 7 days of incubation 80% pure granulocyte and 20% mixed granulocyte‐macrophage colonies were found in the presence of both stimuli. However, on day 14 the incidence of granulocyte‐macrophage colonies increased to 60%, whereas the percentage of pure granulocyte colonies decreased to 20%. The frequency of eosino‐phil colonies was relatively low (median 15%) with both stimuli. The described system can be applied successfully for studies of myelopoiesis in vitro. Both sources of colony stimulating activities (CSA) employed had no significant difference in their ability to stimulate mye
ISSN:0737-1454
DOI:10.1002/stem.5530010507
出版商:Wiley Subscription Services, Inc., A Wiley Company
年代:1983
数据来源: WILEY
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