摘要:

AbstractThe cloning of established human leukemia and lymphoma cell lines at limiting dilutions in microwells and soft agar is described. Growth of most cell lines was improved by the addition of human AB serum and irradiated human bone marrow stromal cells. In general, the cloning efficiency in microwells was greater than in soft agar. The effect of bone marrow stromal cells appeared to be caused by a diffusable factor(s), but close cellular interaction could not be excluded since cloning in microwells produced consistent and optimal cell growth compared to growth in soft agar. It was concluded that cloning of leukemia and lymphoma cell lines in microwells was the preferred method and that similar techniques could improve the cloning of fresh leukemia and lymphoma cells.

ISSN:0737-1454    

DOI:10.1002/stem.5530020501

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530020502

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractUsing monoclonal antibody and fresh rabbit serum, mononuclear cells from 11 healthy individuals were depleted of either helper or suppressor T‐lymphocytes by complement‐mediated lysis. The effect on T‐lymphocyte colony formation was studied to determine the nature of the T‐colony‐forming cell. While depletion of either subset of T‐lymphocytes simcantly reduced T‐colony formation compared to controls (P<0.01), colony formation did not differ significantly between helper or suppressor T‐cell‐depleted cells. Thus T‐colony formation appears to be a property of both helper and suppressor T‐lymphocytes, and both subsets are necessary for opt

ISSN:0737-1454    

DOI:10.1002/stem.5530020503

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractMonkey bone marrow‐derived adherent cells were maintained in culture for six months. Phase contrast and scanning electron microscopy showed two cell shapes: fusiform and polygonal. No difference was observed in the cyto‐chemical staining of the two shapes. Both stained positively for a‐napthyl acetate esterase, acid phosphatase, collagens I and 111, and lipids and negatively for per‐oxidase and factor VIII antigen. A small proportion (1.5%) were alkaline phosphatase‐positive. An average of 14% of the cells were phagocytic in the primary culture, but this proportion decreased progressively with passage. Fc receptors were not detected, while C3 receptors were detected in 1% of primary cultured cells in primary culture, but were not detected in subcultured cells. Adherent cells were not evident in cultures supplemented with 10 mM ammonium acetate. These findings indicate that monkey marrow‐derived adherent cells are fibroblastoi

ISSN:0737-1454    

DOI:10.1002/stem.5530020504

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractTo test the hypothesis that paroxysmal nocturnal hemoglobinuria (PNH) is a hematopoietic stem cell disorder, the growth of BFU‐e and CFU‐gm and the complement sensitivity of cultured cells from BFU‐e and CFU‐gm colonies, as well as of unipotential progenitor cells (CFU‐gm and BFU‐e), were examined in five PNH patients. BFU‐e growth was reduced in the three patients examined, and poor CFU‐gm growth was noted in three of the five patients. Compared to normals, BFU‐e and CFU‐gm colonies in all patients demonstrated an increased susceptibility to the lytic action of complement when the release of 59Fe and myeloperoxidase was measured as specific markers for monitoring membrane damage. Compared to the growth of normal bone marrow cells, CFU‐gm growth was significantly inhibited by pretreatment of bone marrow mononuclear cells with monoclonal OKIal antibody and complement. These findings support the proposition that a membrane defect predisposing blood cells to complement‐mediated lysis may occur at the level of unipot

ISSN:0737-1454    

DOI:10.1002/stem.5530020505

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractThe effects of two varieties of semisynthetic deoxyharringtonine on the tumor mass and survival time of mice with murine leukemia were evaluated. Compared to untreated control mice, the survival time of those with murine leukemia increased by 146% (L7212) and 68% (L615) after treatment. The LD50for deoxyharringtonine variants DH4.8 and DH was determined to be 322 ± 9 mg/kg and 71 ± 1 mg/kg, respectively. Seventeen patients were then treated with either DH or DH4.8; 11% achieved complete remission, 23% achieved partial remission, and an additional 35% had markedly lower leukocyte levels after treatment. Side effects were minimal. It was concluded that deoxyharringtonine is an effective anti‐leukemic ag

ISSN:0737-1454    

DOI:10.1002/stem.5530020506

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530020507

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY