摘要:

AbstractMixed leukocyte cultures (MLC) were prepared utilizing mouse spleen cells from strains with: (1) major histocompatibility complex (MHC) differences, (2) many non‐MHC gene differences, and (3) only a few non‐MHC gene differences. After 4 days, the conditioned media (CM) of MLC from (1) and (2) above were found to contain high granulocyte/macrophage colony‐stimulating activity (CSA) when assayed in methylcellulose clonal cultures. Also the stimulation index (SI) of CSA was elevated on day 4 of incubation, even though3H‐Thymidine (3HTdR) incorporation and SI as measured by3HTdR increased at 4 and 6 days, respectively. However, MLC‐CM from mouse strains with only a few non‐MHC gene differences contained low titers of CSA after 4 days in culture, and this was paralleled by a low SI. For MLC with MHC differences, and with many non‐MHC gene differences, the response detected by an increase in CSA production appeared more promptly and was perhaps more sensitive when compared with3HTdR incorporation in the mixed leukocyte reaction (MLR). From these results, we conclude that the presence of CSA in MLC‐CM at 4 days of culture represents a good marker for the degree of MLR, and we postulate that the CSA titer in MLC‐CM may be relevant for the prediction of graft‐versus‐

ISSN:0737-1454    

DOI:10.1002/stem.5530010201

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

摘要:

AbstractOur fundamental understanding of adipose tissue kinetics has, in recent years, been advanced by the finding that there exists in the white adipose tissue, a population of stem cells which under appropriate conditions can differentiate and mature into adipocytes containing lipid inclusions. The evidence for the presence of this stem cell population is derived from both in vivo and in vitro studies.

ISSN:0737-1454    

DOI:10.1002/stem.5530010202

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

摘要:

AbstractThe effect of agar sterilized by either boiling or autoclaving on human melanoma colony formation in soft agar was compared using cells from 17 biopsies of metastatic malignant melanoma. The frequency of colony formation was significantly increased for cells grown in boiled agar in 8 samples (47%), unchanged in 8 samples (47%), and decreased in only one sample (6%). There were increases in both cluster and colony formation for the melanomas which had augmented colony formation when grown in boiled agar. There was also qualitative morphological improvement, including rounder, smoother cells and less extracellular debris surrounding the colonies. These data suggest that melanoma colony formation is enhanced when cells are grown in agar which has been sterilized by boiling rather than autoclaving.

ISSN:0737-1454    

DOI:10.1002/stem.5530010203

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

摘要:

AbstractThe CFU‐Gm content of liver, spleen and bone marrow of human fetuses was determined from the 8th to 28th week of gestational age. The study of progenitor cells from 43 fetal livers revealed that the CFU‐Gm increased from 8 to 12 weeks, was relatively stable through 16 weeks and thereafter declined by 22 to 24 weeks. The total number of liver CFU‐Gm rose from 104to greater than 106CFU‐Gm by the 16th week of gestation and declined to approximately 4 × 105progenitor cells by 22 to 24 weeks. Peak values of bone marrow CFU‐Gm were noted at 10 to 14 weeks of gestation. Generally, the numbers of these progenitor cells declined by 16 to 18 weeks; however, several high values were obtained at later times. Splenic CFU‐Gm were relatively constant in number between 12 and 28 weeks of gestation with no correlation with fetal age. Thus, these studies indicate that certain fetal tissues contain substantial numbers of hemopoietic progenitor cells. Based on the quantity of CFU‐Gm obtained, it appears feasible to use fetal liver cells as a source of progenitor cells for t

ISSN:0737-1454    

DOI:10.1002/stem.5530010204

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

摘要:

AbstractA new hematopoietic cell line, designated KCL‐22, was established in vitro by cultivation of pleural effusion cells obtained from a woman with chronic myelogenous leukemia in blast crisis. KCL‐22 grew in suspension culture with a doubling time of 24 h and consisted of immature undifferentiated cells which were positive for periodic acid‐Schiff and acid phosphatase staining. Chromosome analysis of the KCL‐22 line showed a female karyotype with double Ph1chromosomes and additional chromosome abnormalities. Its representative karyotype was 52, XX, + 1p‐, + 6, + 8, + 8, + 8, t(9q +; 22q‐), + 22q‐. This cell line possessed receptors for the Fc portion of IgG, but lacked lymphoid cell characteristics and the Epstein‐Barr virus‐associated nuclear antigen. These results indicate that the KCL‐22 cells were derived from chronic myelogenous leukemia cells. This cell line should prove useful for research involving various aspects of chronic m

ISSN:0737-1454    

DOI:10.1002/stem.5530010205

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

摘要:

AbstractThe effects of intralesional chemotherapy with 7 different drugs on line 10 hepatoma grown in strain 2 guinea pigs were compared with the sensitivity of line 10 tumor cells in vitro, using a micro modification of the tumor stem cell assay with capillary tubes. A modified method was used to evaluate the in vitro dose‐response curves. The correlation for in vivo/in vitro resistance was found to be 100% and for in vivo/in vitro sensitivity it was 80

ISSN:0737-1454    

DOI:10.1002/stem.5530010206

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530010207

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1983

数据来源: WILEY