ISSN:0737-1454    

DOI:10.1002/stem.5530060201

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530060202

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractThe colony‐forming efficiency (CFE) of primary human tumor cells cultured in the adhesive‐tumor‐cell culture system (ATCCS) using Ham's F12 (F12) or Eagle's minimum essential medium, alpha modification (alphaMEM) and culture medium supplemented with either swine, equine or bovine sera were compared. AlphaMEM supplemented with equine serum provided the highest CFE of the combinations. The CFE increase due to the change from F12 to alphaMEM was approximately 5‐fold, and the increase due to the change in serum from swine to equine was approximately 2‐fold. Cytokeratin staining showed that this increase was not due to fibroblast growth. The high‐average CFE with alphaMEM, approximately 3%, means that an inoculum of only 2 × 103cells is needed to achieve formation of approximately 65 colonies in control cultures, thereby increasing the performance of this system when used in a chemosensi

ISSN:0737-1454    

DOI:10.1002/stem.5530060203

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractPrevious studies have shown that a subset of bovine ovarian granulosa cells can proliferate to form clones of functional cells in suspension in a semisolid agar matrix, without the requirement for attachment to the substratum. These clonogenic anchorage‐independent granulosa cells are responsive to epidermal growth factor and exhibit properties of a primitive progenitor cell population. We have used this assay system to analyze the proliferation of granulosa cells during ovarian follicular maturation. As the follicle increases in size, there is a progressive decline in the ability of granulosa cells to clone in agar, and the proliferative potential of these cells as measured by colony size also decreases. The ratio of large colonies with high proliferative potential (>250m̈m in diameter)to small colonies with limited capacity for growth falls from 1.92 in follicles less than 7 mm in diameter, to 0.32 in follicles larger than 10 mm in diameter. This occurs as the follicular content of granulosa cells with more limited capacity for clonal growth in agar undergoes expansion. Analysis of colony‐forming cells in follicles harvested at early and late estrus suggests that these cells are regulated by complex intraovarian factors rather than circulating gonadotropin levels. Our data indicate that the granulosa cell lineage is an age‐structured continuum of maturing and differentiating cells with a progressively restricted proliferative ca

ISSN:0737-1454    

DOI:10.1002/stem.5530060204

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractA competitive repopulation assay utilizing chromosome markers was used to assay the reconstituting potential of hematopoietic populations. The test populations consisted of tibial murine marrow locally irradiated with doses ranging from 1.5 Gy to 8.5 Gy and of marrow generated from either murine splenic or marrow stem cells. The purpose of this assay was to assess the innate proliferative potential and microenvironmental influences on the ability to repopulate.Regardless of origin, spleen repopulating ability consistently agreed with spleen colony‐forming unit (CFU‐s) content. Doses of radiation from 5 Gy to 8.5 Gy diminished, by a factor of 2, the ability to repopulate marrow despite maintenance of CFU‐s levels. Marrow generated from splenic stem cells had one‐fifth the repopulating ability of marrow derived from marrow stem cells, even though CFU‐s levels were equivalent. The results imply that the splenic environment can only maintain stem cells at the level of the CFU‐s, even if the stem cells were originally of higher quality, and that their original potential cannot be regained in a marrow environment. Nevertheless, the marrow can maintain more primitive stem cells, but this reserve is drained to support C

ISSN:0737-1454    

DOI:10.1002/stem.5530060205

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractWe previously reported the isolation of an adherent murine marrow cell line termed TC‐1, and the initial characterization of two subclones derived from this line. In this study we report a further characterization of two subclones from the non‐cloned TC‐1 cell line. One subclone, TC‐1‐C‐3, consisted of large, slow‐growing syncytial polypoid cells that grew to relatively low saturation densities, did not form colonies in soft agar and showed desmosome‐like junctions. The other subclone, TC‐1‐C‐U, consisted of smaller, rapidly growing fibroblast‐like diploid cells which showed anchorage‐independent growth in soft agar. Both these subclones produced growth factors which stimulated giant macrophage colonies in soft agar culture in vitro, but only the TC‐l‐C‐3 subclone produced a retrovirus, whose source was most likely the endogenous ecotropicEmv‐2provirus present in chromosomal DNA in C57BL mice. This retrovirus from the TC‐1‐C‐3 subclone did not appear capable of transforming TC‐1‐C‐U cells. Together, these data suggest that TC‐1‐C‐3 cells have a special capacity for supporting hemopoiesis. The question of whether the mechanism of this support relates to an intrinsic property of the cell or is possibly

ISSN:0737-1454    

DOI:10.1002/stem.5530060206

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

摘要:

AbstractInterleukin 2‐activated lymphocytes (lymphokine‐activated killer [LAK] cells) cultured from 2 to 14 days were added to the cultures of granulocyte precursors (CFU‐g). The LAK cells inhibited colony formation of granulocyte precursors; LAK cells cultured for five days showed the strongest inhibitory activity on colony formation.The presence of cell‐to‐cell interaction between LAK cells and bone marrow mononuclear cells (BMNC) in CFU‐g assays emphasized the LAK cell‐derived colony inhibitory activity (LAK‐CIA), but cell‐to‐cell interaction was not always a requirement for LAK‐CIA, since LAK cells were also found to inhibit colony formation without such interaction. This report shows that LAK cells can inhibit in vitro colony formation of granulocyte precursors. We therefore concluded that the observed CIA is caused by soluble factor(s) derived from LAK cells, and that E‐rosette‐forming cel

ISSN:0737-1454    

DOI:10.1002/stem.5530060207

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530060208

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1988

数据来源: WILEY