摘要:

AbstractTwo possible standard sources of colony‐stimulating factor (CSF) have been available commercially; one is GCT‐conditioned medium (GCT‐CM) and the other is T3M‐SA‐CSF. To clarify their biological activity and determine whether they can provide a stimulus identical with leukocyte feeders, the number and the morphology of colonies stimulated by them were compared. A dose‐response study and a mixing experiment between them suggested the presence of inhibitor(s) in GCT‐CM but not in T3M‐SA‐CSF. Both types of CSF stimulated mainly granulocytic colonies at day 7. T3M‐SA‐CSF caused a dose‐dependent increase in rnacrophagic colonies at day 14. The number of colonies stimulated by GCT‐CM and T3M‐SA‐CSF did not differ from that of leukocyte feeders, but the morphology of colonies differed significantly. These results indicate that there is a qualitative difference in the effect of GCT‐CM and T3M‐5A‐CSF on human bone marrow cells, and that they do not supply a stimulus identical with leukocyte feeders. Though GCT‐CM and T3M‐SA‐CSF are expected to serve as useful tools in the standard CFU‐C assay, we should observe their characteristics and accumulate more information about their behavior on nor

ISSN:0737-1454    

DOI:10.1002/stem.5530020401

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractWe studied the effect of lithium on diffusion chamber (DC) granulopoiesis. When DC loaded with bone marrow cells were implanted into the peritoneal cavity of mice previously injected with lithium carbonate, more pro‐liferative and nonproliferative granulocytes were produced as compared to DC implanted into control hosts. The number of DC CFU‐c was increased significantly in the lithium‐treated group, but there was no difference in the number of DC CFU‐s. Levels of DC fluid CSF showed no evident correlation with DC myelopoiesis. These data suggest that a humoral factor other than CSF mediates the action of lithium in DC granulopoiesis, and that lithium's influence on DC hematopoietic stem cell proliferation occurs mainly at the CFU

ISSN:0737-1454    

DOI:10.1002/stem.5530020402

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractWe studied the effects of 1,25‐dihydroxyvitamin D3 and other metabolites of vitamin D3 on the maturation in liquid culture and on colony formation in semisolid media of marrow and buffy coat cells from patients with myeloid leukemias and from normal individuals. After incubation with 1,25‐dihydroxyvitamin D3, a proportion of both normal and leukemic myeloid cells resembled cells of the monocyte‐macrophage lineage; these cells expressed alpha‐naphthyl‐acetate esterase and were able to phagocytize and killcandidaorganisms. When granulocyte‐macrophage progenitor cells (CFU‐GM) were incubated with 1,25‐dihydroxyvitamin D3, the number of monocyte‐macrophage colonies was increased and the number of granulocyte colonies was reduced; megakaryocyte colony formation (CFU‐Mk) was inhibited substantially; and there was no effect on erythroid (BFU‐E) or multilineage (CFU‐GEMM) progenitor cell colony formation. We propose that 1,25‐dihydroxyvitamin D3may induce cells that are normally committed to differentiate along the granulocytic pathways to differentiate instead along the monocyte‐macrophage pathway. If these in vitro observations reflect the in vivo activity of 1,25‐dihydroxyvitamin D3, it may be involved in the modulation of collage

ISSN:0737-1454    

DOI:10.1002/stem.5530020403

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractHeterotransplantation was performed to study the maturational capacity of a new Ph1 chromosome‐positive chronic myelogenous leukemia cell line (KCL‐22). Five million KCL‐22 cells of undifferentiated blasts were implanted intraperitoneally into 15 immunosuppressed newborn hamsters. Of the 15 hamsters, 10 that survived developed granulocytic tumors 16–61 days after implantation. The tumor cells were composed of a mixed population of undifferentiated blasts, neutrophils, and eosinophils and had a human female karyotype with double Phlchromosomes identical to that found in the KCL‐22 cell line used for transplantation. These findings indicate that Ph' chromosome‐positive cells can be induced to mature along the neutrophil‐eosinophil lineage after growth in the heter

ISSN:0737-1454    

DOI:10.1002/stem.5530020404

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractWe determined the chemotherapeutic susceptibility of normal human granulocyte progenitor cells (CFU‐C) and acute myelogenous leukemia cells (HL‐60) in co‐culture. Nucleated bone marrow cells and HL‐60 cells were mixed in 0.3% agar containing McCoy's 5A medium, fetal bovine serum, human placenta‐conditioned medium, and various concentrations of chemotherapeutic agents. They were incubated in 5% humidified CO2at 37°C for 8–10 days. CFU‐C and HL‐60 colonies were differentiated morphologically. The formation of CFU‐C was progressively inhibited with the increasing number of HL‐60 cells, whereas the presence or absence of bone marrow cells did not influence the number of HL‐60 colonies. In separate culture, HL‐60 cells were more sensitive to vincristine than were CFU‐C cells. In co‐culture, however, the CFU‐C became more sensitive to vincristine than in the separate culture. Similarly, co‐culture CFU‐C were more sensitive to daunorubicin than in separate culture. These data indicate that HL‐60 leukemic cells exert inhibitory effects on normal bone marrow CFU‐C; in such an inhibited state, normal bone marrow is more suscepti

ISSN:0737-1454    

DOI:10.1002/stem.5530020405

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

摘要:

AbstractImmunologically mediated aplastic anemia (AA) in mice was induced by the i.v. injection of 107lymph node cells (LNC) from H‐2kidentical but Mls mismatched CBA/J donor mice into previously irradiated (600 rad total body gamma) C3H/HeJ mice. Cyclosporin A (CsA), 25 mg/kg, was administered sub‐cutaneously from day ‐1 to day 30. Control mice included C3H/HeJ mice which received 600 rad alone, C3H/HeJ mice which received 600 rad plus CsA as above, and C3H/HeJ mice which received 600 rad total body irradiation followed by 107LNC from CBA/J donors. CsA failed to prevent lethal AA. These results suggest that the pathogenetic mechanisms operating in immunologically mediated AA differ from the mechanisms operating in rodents transplanted with allogeneically mismatched marrow or spleen cells which develop graft‐versus‐host disease. The results are consistent with a non‐T cell‐dependent mechanism c

ISSN:0737-1454    

DOI:10.1002/stem.5530020406

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530020407

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY

ISSN:0737-1454    

DOI:10.1002/stem.5530020408

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1984

数据来源: WILEY