摘要:

A diphtheria toxin producing strain of “C. ulcerans” was isolated from the throat of a 9‐year‐old boy who was previously immunized against diphtheria. This is the first reported case of human infection due to this organism in

ISSN:0108-0180    

DOI:10.1111/j.1699-0463.1987.tb03139.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The specificity of conventional, adsorbed antisera against agglutinogens 1, 2, and 3 ofBordetella pertussiswas examined by slide agglutination and by agglutination in microtitre wells. Unadsorbed hyperimmune sera showed higher agglutinating activity against autologous or homologous cells than against cells of heterologous serotype. Adsorption of sera with heterologous cells increased the serotype specificity considerably. In spite of extensive adsorption, these anti‐agglutinogen sera were still found to cross‐react withB. parapertussisand/orB. bronchisepticastrains. Adsorption experiments withB. pertussishyperimmune sera against serotype 1‐, 1.2‐, and 1.3‐organisms demonstrated that the cross‐reacting surface antigens differed from the agglutinogens 1, 2, and 3. Thus, in making species‐specific reagents for diagnostic use it may be of value to include adsorption withB. parapertussisandprobablywithB. bronchiseptica.Limited data indicated that there is no need to useB. aviumfor adsorption. The agglutination assays were also used to test three monoclonal antibodies stated to be specific for the agglutinogens 1, 2, and, 3, respectively. Some anomalous behaviour for the anti‐agglutinogen 1 reagent was found, whereas the anti‐agglutinogen 2 and 3 reagents corresponded well with the present polyc

ISSN:0108-0180    

DOI:10.1111/j.1699-0463.1987.tb03140.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Antigen detection for diagnosis of pneumococcal infections has earlier been based on the use of antibodies against capsular antigens. Methods based on the demonstration of C‐polysaccharide (PnC) have the advantage of using antibodies against one single species‐specific antigen instead of applying a polyvalent mixture of antisera against 83 different capsular antigens. Very little has been done earlier to evaluate the accessibility of the PnC on the surface of pneumococcal cells, particularly cells carrying capsules, and the release of PnC to the environment. We have used a monoclonal anti‐PnC antibody in an ELISA inhibition test to demonstrate PnC during growth from four differentStreptococcus pneumoniaestrains (capsular types 1, 3 and 19F) and a C‐mutant strain, reported to carry PnC as a small capsule. Heavily‐capsulated types 3 and 19F exposed more PnC on their surface than the type 1 strain, and considerable amounts of PnC were released to the culture medium during growth. The C‐mutant strain differed from the other strains in that it exposed less PnC on its surface. The type 1 strain and the C‐mutant released approximately the same amount of PnC to the culture medium. Treatment with antibiotics during growth caused a decrease in surface‐located PnC but did not significantly affect the amount released. The total accessible PnC in these cultures was quite significant and well above the limit of detection in an ELISA earlier described for the detection of PnC in clinical samples. The results presented here give support to the notion that the demonstration of PnC in clinical samples should provide a good basis for diagnosis of pneumoc

ISSN:0108-0180    

DOI:10.1111/j.1699-0463.1987.tb03141.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY