摘要:

SummaryLymphocytes from 103 Holstein cattle were tested with 11 antilymphocytotoxic sera. Four of these sera were produced by whole blood immunization; these generally yielded lymphocytotoxicity against a large number of animals in each test panel. The majority of the sera were collected from parous cows which had never been immunized. Observations about this latter group of sera are (1) lymphocyte antibodies may occur by alloimmunization in foetal‐maternal interaction, (2) normal sera from non‐immunized parous cows generally had a narrower specificity of antibodies than from parous cows immunized with blood from unrelated animals and in some cases these antisera may be monospecific, and (3) these sera from parous cows are easy to obtain without the need for laborious immunizations. Analysis of these lymphocytotoxic sera indicate several reagents were obtained which possessed different antibodies; these were useful in detecting polymorphism of cattle lymphocyte antigens. Using these 11 sera, an analysis of the reactions patterns among 103 Holsteins, including parent‐offspring data resulted in the postulation of several alleles. These data suggest that cattle lymphocyte antigens are very polymorphic and inhe

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1977.tb01647.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

SummaryThe erythrocyte antigen (Ecl) of cattle has been isolated using DEAE‐cellulose chromatography, Sephadex gel filtration, and isoelectrofocusing. The purified antigen is free from hemoglobin and migrates as a single protein on polyacrylamide gels. It exhibits an absorption maximum at 278 nm and has a molecular weight of about 100 000; its isoelectric point is 4.95. The antigen has a carbohydrate moeity and loses its activity after oxidation with periodate. The evidence suggests that it is not a common isozyme, but its exact identity remains unknow

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1977.tb01648.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

SummaryIsoenzyme patterns and the polymorphism of lactate dehydrogenase (LDH) were investigated in 3 fish species of family Cyprinidae, i.e. tench (Tinea tinea), crucian carp (Carassius carassius) and carp (Cyprinus carpio).The isoenzyme patterns were tissue and species specific. In crucian carp subunits with different electro‐phoretic mobility are present, which are genetically controlled from the B1, B2, A1, A2and C loci, while the set of loci in carp is B1, B2, A, C1and C2and in tench B, A, C. The locus B of LDH in tench, the locus B2in crucian carp, and the loci B1, C1and C2in carp are polymorphic and have two different alleles in each case. The polymorphism did not affect the total LDH activity in the tissues. All the populations investigated were in Hardy‐Weinberg equilibrium. The genetic control of the polymorphism in B1and C1loci in carp was proved by test matings. The polymorphism in B loci tested in erythrocytes may be utilized as genetic markers in the fish breed

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1977.tb01649.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

SummaryIn this report the linear order of genetic determinants for the blood group system B of cattle has been worked out in more detail. Two groups of antigenic specificities — inclusion groups — showing serologic relationship are described. Recombination frequencies do suggest that these specificities are controlled by two clusters of genetic determinants. The structures of these inclusion groups and clusters are compared with those of antigenic complexes in other species, especially in mice. Several similarities and also dissimilarities between the blood group system B of cattle and the H‐2 system in mice are desc

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1977.tb01650.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

SummaryA system is described for freeze drying and storage of reagents (antisera) used in cattle blood typing tests. Reagents were freeze‐dried in evacuated bottles at the desired dilution for rapid reconstitution to use in test procedures. Reagents not in current use were dried in bulk lots and stored in polyethylene film bags. All freeze drying procedures were performed with readily available commercial equipment. A computer program was developed to produce a current inventory of reagent supplies and projections of the number of samples which can be type

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1977.tb01651.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

摘要:

SummaryA genetic polymorphism of the pig amylase 2 system is described. It is controlled by two codominant genes,Am 2AandAm 2B.A comparison of some breeds from Byelorussian breeding farms gives the following frequencies for the allelesAm 2AandAm 2B, respectively: 0.35 and 0.865 in Large White (n = 682), 0.257 and 0.743 in Byelorussian Black and White (n = 400), 0.540 and 0.460 in Hampshire (n = 51).While studying the genotype distribution according to the Hardy‐Weinberg law, a genetic imbalance in Byelorussian Black and White pigs was established (χ2= 56.4). Genetic relationship between theAm 1andAm 2loci was not fou

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1977.tb01652.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1977.tb01653.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1977.tb01654.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1977.tb01655.x

出版商:Blackwell Publishing Ltd    

年代:1977

数据来源: WILEY