摘要:

SummaryThe major bovine serum isoamylases controlled by theAmIlocus have been examined by gel filtration. On Sephadex G‐200 the isoamylases can be resolved into two classes. The AmI A and AmI B have apparent molecular weights of 307 000 daltons whilst the AmI C isozyme has an apparent molecular weight of 44 400 daltons. The separation of the isozymes into two classes according to their elution behaviour on Sephadex G‐200 has been shown to be an affinity separation. All three AmI isozymes are eluted from a non‐dextran media (BioGel A1.5m) with apparent molecular weights of 417 000 daltons. The affinity separation on Sephadex G‐200 has been shown to be inhibited by the addition of 1% (w/v) maltose to the elution buffer. In the presence of 1%(w/v) maltose all three AmI isozymes are coeluted from Sephadex G‐200 with apparent molecular weights of 321000 daltons. The maltase and amylase activities of the AmI isozymes were eluted coincidentally under all the condition

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1982.tb01045.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SummarySera from about 1000 cows were tested for cytotoxicity against a panel of up to 100 lymphocyte samples. Cytotoxic antibodies presumably resulting from trans‐placental immunization of the cow by her calf were found in about 45%of these sera. The antibody titers of sera from parous cows rarely exceed 42, some persisted for over one year, but decreased notably at calving. Thirty‐five immune sera were also produced by alloimmunization with lymphocytes. They usually reached peak titers of up to 44at 2 or 3 weeks after the initial immunization. Subsequent immunizations produced sera with very high titers but they were much more polyspecific. High‐titered antibodies were also produced by skin graft recipients. Useful cytotoxic antibodies were found in 19 of 111 colostrum whey samples. Studies on 13 dam‐calf pairs showed that the newborn calf may acquire cytotoxic antibodies from its mother's colostrum, but the only cytotoxic antibodies detectable in this calf s serum are those not directed against its own lymphocyte antigens. It is concluded that efficient lymphocyte typing requires antibodies from a variety of

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1982.tb01046.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SummaryWe describe a technique to produce high‐titered bovine lymphocytotoxic anti‐sera using skin implants. The main advantage of this technique is that the skin does not need to be processed prior to implantation and no surgical skill is required. In addition, the skin can be stored for up to 2 weeks and can be shipped to other laboratories without special handling and without loss of immunogenic

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1982.tb01047.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SummaryGenetic analysis of a proposed cis‐acting temporal locus (Adh‐3t), which regulates alcohol dehydrogenase C2(ADH‐C2) acitivity in mouse epididymis extracts, among F1(ddN × BALB/c) × ddN male backcross progeny provided evidence for genetic distinctness between the structural (Adh‐3) and temporal (Adh‐3t) loci on chromosome 3. Genetic analysis also confirmed the close, linkage ofAdh‐1(encoding liver and kidney ADH‐A2)and Adh‐3(encoding stomach ADH‐C2) to within 0.3 centimorgans on the mouse genome. Evidence is presented for a proposed closely linked cis‐acting temporal locus (designatedAdh‐1t) for the A2isozyme (encoded byAdh‐1) controlling the activity of this enzyme in mouse kidney extracts, but having no apparent affect on liver and intestine ADH‐A2activities. An extensive survey of the distribution ofAdh‐1, Adh‐3andAdh‐3talleles among 65 strains of mice is reported — with the exception of two Japanese strains (ddN and KF), linkage disequilibrium betweenAdh‐3andAdh‐3twas observed. Sex differences in mouse liver and kidney ADH‐A2activities were observed, with male/female ratios of approximately 0.6 and

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1982.tb01048.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SummaryResearch programs involving the study of genetic variation of proteins have been carried out both in humans and catarrhine primates but rarely in New World species. Considering the great possibilities offered by protein variations as genetic markers in systematic and evolutionary studies in this group of organisms, the authors are specially interested in the investigation of genetic polymorphisms of blood proteins in Amazonian primates. In this paper we describe the electro phoretic patterns of erythrocyte esterases obtained from blood samples of 57Cebus apellaspecimens. Blood hemolysates fromCebusdisplay four main set of bands in azo‐coupled stained gels. These bands are identified as esterases P, A1, A2 and B by their electro phoretic migration, substrate specificity and eserine reaction. The use of the fluoregenic reagent 4‐methylumbelliferyl acetate revealed the presence of a fifth set of enzymes not detected by the azo‐coupled staining method. This ser of enzymes, probably a polymorphic genetic system, was named ESD as in h

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1982.tb01049.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SummaryThe F system of three Danish cattle breeds as determined by four specific anti‐sera is described. In the Jersey breed three alleles are recognised. In the Danish cattle breeds there was no indication of a null allele. However, the phenotypes observed in zebu cattle by means of four reagents suggest the presence of at least six alleles in the bovine F system. Furthermore, the data show that the factors V1and V2do not form a linear subtype system in all cattle breed

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1982.tb01050.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SummaryA two‐colour fluorescence micro cytotoxicity test was used to screen antisera for antibodies specific for bovine B lymphocytes. A total of 114 cattle alloantisera were screened against peripheral blood lymphocytes from 100 unrelated individuals. Anti‐B lymphocyte activity was detected in 47 antisera. Cytotoxic antibodies to antigens other than B lymphocyte specific antigens were removed by absorbing the antisera with buffy coat cells or platelets isolated from whole blood. Selected antisera were used to type paternal half‐sib families. The presence of a polymorphic, MHS‐linked antigen system on B lymphocytes was demonstrated. The tissue distribution and MHS linkage of these antigens suggests this system is analogous to the class II or Ia antigens of other

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1982.tb01051.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1982.tb01052.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY

摘要:

SummaryTo investigate genetic variation and to compare it with those of domestic quail populations, enzymatic and non‐enzymatic proteins in a wild quail population were examined electrophoretically.The wild quail population had a genetic variability considerably lower than those of domestic populations. The average heterozygosity estimated for 22 loci was 0.090 in the wild quail populatio

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1982.tb01053.x

出版商:Blackwell Publishing Ltd    

年代:1982

数据来源: WILEY