摘要:

SummaryLinkage at a recombination frequency of 0.10 or less between the bovine major histocompatibility system and the B, C and L red blood cell groups and the albumin, haemoglobin and transferrin loci was excluded by Morton's lod score method. The white blood cell antigen CA19, which is independent of the bovine majar histocompatibility system, is the J blood group.

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1984.tb01123.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

SummaryTwo‐dimensional agarose gel (pH 8.6)‐horizontal polyacrylamide gel (pH 9.0) electrophoresis of horse serum proteins revealed genetic polymorphism of ceruloplasmin (Cp) and two unidentified serum proteins tentatively designated serum protein 1 (SP1) and serum protein 2 (SP2). Family data were consistent with the hypothesis that the observed Cp and SP1 phenotypes were each controlled by two co‐dominant, autosornal alleles. The three common SP2 phenotypes were shown to be controlled by two codominant, autosomal alleles. Population data and limited family data indicated the occurrence of two additional SP2 alleles. Altogether more than 600 horses representing 13 different breeds were typed for Cp, SP1 and SP2, and allele frequency estimates were calculated.SP2was highly polymorphic in all breeds studied whereasSP1andCpshowed quite low degrees of polymorphism.SP1polymorphism was observed in seven breeds whileCppolymorphism was observed only in the Icelandic toelter horse

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1984.tb01124.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

SummaryLinkage analysis between size variants of the centromeric region of chromosome 15 and G blood group alleles produced a lod score of 5.03. The maximum likelihood estimate of the recombination fraction is θ= 0.24 with a 95 % confidence interval of 0.08<θ<0.40. Since this is the second time that linkage between a chromosome 15 marker and the G blood group locus has been shown, the assignment of the G blood group locus to chromosome 15 is confirme

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1984.tb01125.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

SummaryThe bovine J blood group substance exists as a glycosphingolipid (ceramide deca‐hexoside as well as ceramide dodecahexoside) and as a glycoprotein. The lipidic form occurs in erythrocyte membranes, both forms are found in serum. The lipidic J substances were isolated from erythrocytes and from serum, and identified by thin‐layer chromatography with lipidic J substances isolated from spleen. The glycoprotein nature of the non‐lipidic J of serum was evident by pronase‐catalysed hydrolysis yielding J‐active glycopeptides of lower molecular weights. The lipidic J was completely extracted from lyophilized stroma with chloroform/methanol. From lyophilized serum, however. it was completely extracted only in the presence of water, indicating different binding partners in serum and in erythrocyte membranes.The J lipid was incorporated as intact molecule into the erythrocyte membrane by a simple incubation technique. The incorporation was inhibited by various glyc‐erophospholipids (called blockers). The J glycoprotein could not be transferred to the erythrocyte membrane.Three methods are descrjbed which are suitable for the preparation of a blocker‐free fraction enriched with J lipids from J

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1984.tb01126.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

SummaryThe second generation (n= 227) of British Landrace pigs from selected halothane‐positive parents (36 litters) were blood‐typed for theS(A‐0), HandPhiloci and subjected to four 5‐minute halothane tests at 21, 35, 49 and 63 days of age. Cumulative scores based on seventy and speed of reaction were analysed in relation to single‐locus blood group genotypes and linkage group sequences at two and three loci. A highly significant negative correlation (r =‐0.79) was found between severity and speed of reaction. Significant differences occurred between blood group genotypes and linkage groups in both severity and speed of reaction. Genotypes Ss/s, H a/aorH a/‐andPhi B/Band linkage groups involving these three types had the highest cumulative reaction score and the fastest reaction time, whereas genotypesPhi A/B, S S/SorS S/sandH a/cdand linkage groups with these types had the Iowest and slowest reaction scores. Some differences between genotypes and linkage groups were attributed to phenotypically halothane‐positive parents and offspring being genotypicallyHal N/n. These effects could result from linkage with heterozygous types such asH a/cdand S S/s. The possible role of theHcdallele acting as a genetic marker for a suppressor gene to the halothane reacti

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1984.tb01127.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

SummaryBenzidine staining of starch gels after electrophoresis of sera to which haematin was added revealed polymorphism of haemopexin in sheep, mouflon and goat. In sheep three phenotypes were observed, Hpx A, Hpx AB and Hpx B. Pedigree data support the hypothesis of codominant inheritance from a single locus by two alleles,HpxAandHpxB. Neuraminidase treatment of haemopexin preparations showed that Hpx B covered two variants, B1 and B2, thus indicating genetic control by three alleles (HpxA, HpxB1andHpxB2). In sheep populations the frequency ofHpxBis low. In mouflon, in addition to the two variants that are like those of sheep, absence of haemopexin was observed in some animals, by using starch gel electrophoresis as well as immunoelectrophoresis. In goat, three phenotypes were detected, Hpx A, Hpx AB and Hpx B, differing in migration from those of sheep. Haemopexins of the studied species are heterogeneous. Sialic acid is responsible for electrophoretic heterogeneity of sheep haemopexin. Chemical. composition (amino acid and carbohydrate), molecular weight (56 060) and N‐terminal sequence (Leu‐Pro‐Pro‐) of sheep haemopexin were also det

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1984.tb01128.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

SummaryA comparison is made of single components of the homozygous variants A and D2of bovine serum transferrin by tryptic, chymotryptic and cyanogen bromide digestion. It is concluded that there are three substitutions A:D2‐ Glu:Asp, Lys: Arg and Asp:Gly. In the light of the recent work of Brocket al. (1980) it is concluded that all three substitutions occur in the C‐terminal sequence of the chain. By homology with the sequence of human serum transferrin (MacGillivray et al., 1982) the Lys:Arg and Asp:Gly substitutions probably occur at residues 527 and 446, respectively, from theN‐terminus. The Asp:Gly substitution is considered more likely than our earlier conclusion (Maeda, McKenzie&Shaw, 1977) that there is a deletion in the chain of D2(A:D2, Asp: —). The location of the Glu:Asp substitution is no

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1984.tb01129.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

SummaryGlycopeptides are isolated from subtilisin and pronase digests of whole bovine serum transferrin A and D2. The two variants yield glycopeptides with identical ami‐no acid composition. Hence, there is probably no amino acid substitution in this region of the peptide chain. Amino acid sequence determination of one glycopeptide (subtilisin glycopeptide 8) gives the sequence: (CHO)Asn‐Ser‐Ser‐Leu‐Cys. This sequence is identical with that of residues 491–495 of the sequence for human serum transferrin (MacGillivray et al., 1982) except that in the bovine transferrin, Asp is replaced by Asn, enabling carbohydrate attachment. A second glycopeptide sequence Arg‐(CHO)Asn‐Ala‐Thr‐Tyr is observed, and the significance discussed in relation to carbohydrate moieties of

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1984.tb01130.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1984.tb01131.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

ISSN:0003-3480    

DOI:10.1111/j.1365-2052.1984.tb01132.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY