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11. |
Dynamic adsorption and desorption of proteins |
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Die Makromolekulare Chemie,
Volume 9,
Issue S19851,
1985,
Page 85-98
Yasushi Tamada,
Yoshito Ikada,
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摘要:
AbstractTo study the dynamics of adsorption and desorption of plasma proteins, bovine serum albumin(BSA), γ‐globulin, fibrinogen, and plasma fibronectin(FN) were labeled with131I and phosphate buffered solutions of the single proteins and of the mixed proteins were used for thein vitroadsorption and desorption study. When the proteins in single concentration were first adsorbed onto a polyethylene tube and desorbed after 60 min adsorption by changing the flowing liquid from the protein solution to a buffered saline without any protein, FN molecules were found to remain adsorbed so strongly on the tube surface that their desorption did not take place even after washing with the flowing buffer. On the contrary, BSA was rather readily desorbed from the tube surface upon continued washing. A similar result was obtained when the protein pre‐adsorbed on a polyethylene film was exposed to the solution of the same but unlabeled protein; the adsorbed FN was not replaced, while the BSA solution expelled the adsorbed BSA molecules from the polyethylene surface. Adsorption and desorption behaviors of γ‐globulin and fibrinogen were intermediate between those of BSA and FN. When a mixture of BSA and FN was allowed to flow into the polyethylene tube at a concentration of 0.05 mg ml−1for each protein, BSA adsorption took place more rapidly and significantly than FN in the initial stage of adsorption but was decreased after passing a maximum, whereas FN was increasingly adsorbed onto the polyethylene surface with time and finally surpassed BSA in the adsorpti
ISSN:0025-116X
DOI:10.1002/macp.1985.020091985111
出版商:Hüthig&Wepf Verlag
年代:1985
数据来源: WILEY
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12. |
Preferential adsorption of high density lipoprotein (HDL) in blood plasma/polymer interaction |
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Die Makromolekulare Chemie,
Volume 9,
Issue S19851,
1985,
Page 99-102
A. Bantjes,
W. Breemhaar,
T. Beugeling,
E. Brinkman,
D. J. Ellens,
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摘要:
AbstractA few studies on the adsorption of plasma proteins to polymeric surfaces show that major plasma proteins: albumin (Alb), fibrinogen (Fb) and immunoglobulin (IgG) are adsorbed in much smaller quantities from plasma than from protein solutions (1,2). Present results show that this difference in adsorption is due to the preferential adsorption of high density lipoprotein from plasma onto the material surfaces studied (PVC and PS).
ISSN:0025-116X
DOI:10.1002/macp.1985.020091985112
出版商:Hüthig&Wepf Verlag
年代:1985
数据来源: WILEY
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13. |
Protein‐surface interactions studied by infrared spectroscopy |
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Die Makromolekulare Chemie,
Volume 9,
Issue S19851,
1985,
Page 103-111
M. Houska,
E. Brynda,
J. Kálal,
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摘要:
AbstractInteractions of human fibrinogen and human serum albumin in aqueous buffers with germanium, polyethylene, and carbon planar surfaces were studied by IR internal reflection spectroscopy. The method is shown to be able to monitor continuously and in situ adsorption rates with high time‐resolution (0.5 s), to produce data concerning reversibility of interactions, and to detect surface induced changes of proteins. Albumin and fibrinogen interact with the strongly hydrophobic surfaces irreversibly and fibrinogen interacts irreversibly even during the first 15 s of adsorption. Differences between spectra of the proteins adsorbed and in solution were detected and interpreted as surface induced changes. Anomalously high and partly reversible adsorption of HFb during certain experiments is connected with its denaturation. No effect of thrombin and plasmin on adsorbed HFb was indicated from its spectr
ISSN:0025-116X
DOI:10.1002/macp.1985.020091985113
出版商:Hüthig&Wepf Verlag
年代:1985
数据来源: WILEY
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14. |
Polyethylene/Hydrophilic polymer blends: Surface properties in water |
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Die Makromolekulare Chemie,
Volume 9,
Issue S19851,
1985,
Page 113-117
E. Brynda,
S. P. Novikova,
M. Houska,
N. B. Dobrova,
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摘要:
AbstractPolyethylene/poly(2‐hydroxyethyl methacrylate) and polyethylene/poly(2,3‐dihydroxypropyl methacrylate) blends were prepared as materials for contact with blood. In the course of soaking the blends in distilled water for three months the swelling and contact angles of air bubbles on the surface were measured. The observed decrease in blend‐water interfacial tension was probably due to separation and deposition of hydrophilic polymers at blend‐water interface. Thrombogeneity of the blends in ex‐vivo test decreased with increasing time of soaking
ISSN:0025-116X
DOI:10.1002/macp.1985.020091985114
出版商:Hüthig&Wepf Verlag
年代:1985
数据来源: WILEY
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15. |
Blood compatibility of heparinized polyurethanes |
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Die Makromolekulare Chemie,
Volume 9,
Issue S19851,
1985,
Page 119-124
Peter W. Heyman,
Sung Wan Kim,
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摘要:
AbstractHeparin ‐ immobilized polyurethane (HI‐PU) catheters implanted in canine femoral and jugular veins for one hour periods exhibited significant reduction in thrombus formation compared with untreated PU contralateral controls. PU catheters coated with a 9% heparin dispersion in PU, capable of releasing active heparin for several hours, provided even greater improvement in antithrombogenicity. Both types of heparinized surface displayed reduced platelet aggregation and fibrin formation, although platelet adhesion was somewhat higher on the HI material than on the heparin‐releasing (HR) su
ISSN:0025-116X
DOI:10.1002/macp.1985.020091985115
出版商:Hüthig&Wepf Verlag
年代:1985
数据来源: WILEY
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16. |
Hydrophilic polymeric microspheres : Their use in immunological methods |
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Die Makromolekulare Chemie,
Volume 9,
Issue S19851,
1985,
Page 125-127
L. Fornůsek,
V. Větvička,
J. Zídková,
J. Kopeček,
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摘要:
AbstractHydrophilic polymeric microspheres were prepared by y‐radiation initiated copolymerization of 2‐hydroxyethyl methacrylate, ethylene dimethacrylate, methylenebisacrylamide and methacrylic acid in aqueous solution with possible addition of special monomers bearing fluorescent or other specific ligand groups. The microspheres were used in detection of different subtypes of immunoglobulin Fc‐receptors and complement components receptors as well as in the studies of the process of receptor‐mediated phagocytosis. A simple assay for testing the phagocytic activity of peripheral blood leukocytes is proposed for clinical appli
ISSN:0025-116X
DOI:10.1002/macp.1985.020091985116
出版商:Hüthig&Wepf Verlag
年代:1985
数据来源: WILEY
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17. |
Polyglutamines‐versatile biodegradable carriers |
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Die Makromolekulare Chemie,
Volume 9,
Issue S19851,
1985,
Page 129-135
F. Rypáček,
V. Saudek,
J. Pytela,
V. Škarda,
J. Drobník,
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摘要:
AbstractBiodegradation of homopolymers, poly‐α‐[Nγ‐(2‐hydroxyethyl)‐L‐glutamine] and poly‐α‐[Nγ‐(2‐hydroxypropyl)‐L‐glutamine], and of copolymers, poly‐α‐[Nγ‐(2‐hydroxyethyl)‐L‐glutamine‐co‐L‐phenylalanine] with 3.7, 5.6, and 10.2 mol‐% of Phe units was studied in vitro using pronase E, chymotrypsin A4and extracts from native tissues. Gel permeation chromatography was used for evaluating the molecular‐weight distribution of the original and partially degraded polymers. Homopolymers of Nγ‐(2‐hydroxyalkyl)‐L‐glutamines are biodegradable in the main chain by pronase as well as by native tissue extracts, yielding low‐molecular‐weight products. Although chymotrypsin does not catalyze hydrolysis of peptide bonds in the homopolymers, incorporation of phenylalanine units in the main chain by copolymerization renders the copolymer degradab
ISSN:0025-116X
DOI:10.1002/macp.1985.020091985117
出版商:Hüthig&Wepf Verlag
年代:1985
数据来源: WILEY
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18. |
In vivo and in vitro immunogenicity of water‐soluble haptenated copolymers for mouse and human lymphocytes |
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Die Makromolekulare Chemie,
Volume 9,
Issue S19851,
1985,
Page 137-143
H. Tlaskalová‐Hogenová,
J. Kopeček,
K. Ulbrich,
F. Rypáček,
M. Pospíšil,
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摘要:
AbstractAntibody response to soluble copolymers of N‐(2‐hydroxypropyl)methacrylamide or N‐(2‐hydroxyethyl) aspartamide, of different molecular weights and various content of haptenic dinitrophenyl (DNP) groups in side chains, was studied using the plaque method and enzymelinked immunosorbent assay
ISSN:0025-116X
DOI:10.1002/macp.1985.020091985118
出版商:Hüthig&Wepf Verlag
年代:1985
数据来源: WILEY
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19. |
Morphological foundation of hydrogel use for vascular occlusion |
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Die Makromolekulare Chemie,
Volume 9,
Issue S19851,
1985,
Page 145-150
N. D. Skuba,
J. Kálal,
I. Vacík,
O. S. Voroncova,
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摘要:
AbstractMorphological grounds presented here show, that hydrogel with a 2‐hydroxyethyl methacrylate basis is a suitable material for vascular occlusion with therapeutic purposes. It has been shown, that hydrogel emboli neither injure, nor irritate the blood vessel wall; they promote thrombogenesis in the localization sites and are preserved in the vascular lumen for a period necessary for the manifestation of the therapeutic occlusive effect. The hydrogel property to swell directly in the vascular lumen, as well as to promote blood clotting, is a factor providing for reliable occlusio
ISSN:0025-116X
DOI:10.1002/macp.1985.020091985119
出版商:Hüthig&Wepf Verlag
年代:1985
数据来源: WILEY
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20. |
Specific removal of low density lipoprotein from blood plasma |
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Die Makromolekulare Chemie,
Volume 9,
Issue S19851,
1985,
Page 151-154
N. Maaskant,
A. Bantjes,
H. J. M. Kempen,
D. J. Ellens,
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ISSN:0025-116X
DOI:10.1002/macp.1985.020091985120
出版商:Hüthig&Wepf Verlag
年代:1985
数据来源: WILEY
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