摘要:

An Epstein-Barr virus (EBV) specific monoclonal antibody (MAb), designated 55H3, was produced after immunizing BALB/c mice with 12–0-tetradecanoylphorbol-13-acetate (TPA) activated B95–8 cells. It was possible to demonstrate that this MAb detected an EBV-specific antigen(s) in the EBV genome-positive producer cell lines B95–8 and M-AB A by immunofluorescence. Immunoglobulin prepared from ascites fluid neutralized the activity of the EBV-encoded DNA polymerase, but not the alkaline DNase. By Western blotting, the 55H3 MAb reacts with an 85/80-KD polypeptide. The 55H3 should be useful in examining the role of EBV DNA polymerase in viral replic

ISSN:0300-5526    

DOI:10.1159/000150156

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Presence of cytomegalovirus (CMV) specific immunoglobulin M (IgM) is generally considered strong evidence of current or recent CMV infection. Sera from 110 elderly individuals (age range 61–100 years) were compared with sera from 87 younger individuals (age range 16–47 years). Prevalence of CMV IgM was found to be 30% in the older group (mean age 83 years) and only 6% in the younger group (mean age 29 years). In spite of increased CMV antibody levels, none of the elderly individuals were symptomatic. Viral cultures of urine from 72 elderly individuals were negative for CMV. These results suggest that the presence of CMV-specific IgM must be interpreted with caution in an elderly populat

ISSN:0300-5526    

DOI:10.1159/000150157

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

We reported previously that the administration of anti-α/β murine interferon antibody (anti-IFN-Ab) to C57BL/6J mice genitally infected with herpes simplex virus type 2 (HSV-2) dramatically increases morbidity and mortality. The effect of anti-IFN-Ab is visible early postinfection and it is more evident when low virus doses are used. This report describes our study of the mechanism of action of anti-IFN-Ab. We found that anti-IFN-Ab did not affect peripheral multiplication of the virus at the site of infection. The T-cell cytotoxic activity of spleen and lymph node cells of HSV-2 genitally infected C57BL/6J mice was dependent on the virus dose and peaked 7 days postinfection and, therefore, was excluded as a possible mechanism for the effect of anti-IFN-Ab. Natural killer (NK) cell activity paralleled the effect of anti-IFN-Ab closely; the activity peaked early postinfection, and there was no correlation between NK cell activity and virus dose. We have now observed that in mice treated with anti-IFN-Ab, the NK cell activity was significantly lower than in mice infected and not treated with anti-IFN-Ab. The lack of effect of anti-IFN-Ab on local multiplication of HSV-2 coupled with the decrease in NK cell activity indirectly point to a non-antiviral mechanism of action of endogenous IFN in viv

ISSN:0300-5526    

DOI:10.1159/000150158

出版商:S. Karger AG    

年代:1990

数据来源: Karger