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1. |
Problems Concerning the Taxonomy of the ‘Movar-Type’ Bovine Herpesviruses |
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Intervirology,
Volume 28,
Issue 1,
1987,
Page 1-7
Adorjan Bartha,
Abdalla M. Fadol,
Heinrich Liebermann,
Hanns Ludwig,
Sashi B. Mohanty,
Fernando A. Osorio,
David E. Reed,
John Storz,
Otto C. Straub,
M.J. Van der Maaten,
G. Wellemans,
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摘要:
The inconsistency in naming and labeling bovine herpesviruses (BHVs), other than BHV types 1 and 2 (BHV-1 and BHV-2), found in the literature is reviewed. To resolve the confusion and misunderstanding caused by the use of BHV-3, BHV-4 and BHV-5 for the same kind of BHVs, the most used label BHV-4 is proposed for designating Movar-type BHVs (which also were named Orphan viruses’ or ‘cytomegaloviruse
ISSN:0300-5526
DOI:10.1159/000149991
出版商:S. Karger AG
年代:1987
数据来源: Karger
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2. |
Epizootic Guinea Pig Herpes-Like Virus Infection in a Breeding Colony |
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Intervirology,
Volume 28,
Issue 1,
1987,
Page 8-13
Beverly L. Connelly,
Gary L. Keller,
Martin G. Myers,
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摘要:
A breeding colony of strain-2 guinea pigs which had been relatively free of indigenous caviid herpesviruses experienced an explosive outbreak of guinea pig herpes-like virus apparently as a consequence of intermixing groups and contamination of the water supply. A new breeding colony has been established and has been maintained apparently free of recognized caviid herpesviruses.
ISSN:0300-5526
DOI:10.1159/000149992
出版商:S. Karger AG
年代:1987
数据来源: Karger
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3. |
Immunogenicity of the Gene S and Pre-S Domains in Hepatitis B Virions and HBsAg Filaments |
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Intervirology,
Volume 28,
Issue 1,
1987,
Page 14-25
Klaus-Hinrich Heermann,
Frank Kruse,
Maria Seifer,
Wolfram H. Gerlich,
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摘要:
The three morphological forms (20-nm particles, filaments, virions) of hepatitis B surface antigen (HBsAg) were isolated from serum of chronic virus carriers or from transfected cell lines. BALB/c mice and guinea pigs were immunized with the antigens and the antibody responses against the three antigenic domains of the viral envelope were assayed. The proportion of pre-S1, pre-S2 and gene S antibodies was similar to the molar proportion of the domains in the immunogens. The major gene S and pre-S1 epitopes were conformational, and the major epitopes of the pre-S2 domain were sequential. The immunogenicity of natural and recombinant antigens was identical. The proportion of subtype-specific antibodies was high. The results suggest that recombinant HBsAg filaments containing both subtypes ad and ay may be optimal hepatitis B vaccines.
ISSN:0300-5526
DOI:10.1159/000149993
出版商:S. Karger AG
年代:1987
数据来源: Karger
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4. |
Characterization of Cloned Measles Virus mRNAs by in vitro Transcription, Translation, and Immunoprecipitation |
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Intervirology,
Volume 28,
Issue 1,
1987,
Page 26-39
Karl W. Hasel,
Sharon Day,
Stewart Millward,
Christopher D. Richardson,
William J. Bellini,
Peter A. Greer,
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摘要:
A cDNA library was constructed from poly(A)+ RNA prepared from VERO cells infected with the Edmonston strain of measles virus. Clones corresponding to five major viral-specific transcripts were identified by northern blot hybridization analysis. Probes prepared from these five clones detected an additional five minor viral RNA transcripts. The sizes and hydridization patterns of these minor RNA species are consistent with their being bicistronic transcripts arising from a viral genomic template with the gene order 3’. NP-P/C-M-F-HA-(L).. 5’. To assess the coding capacity of these cDNA clones they were inserted into pSP64, transcribed in vitro, the RNA was translated in reticulocyte lysates, and the protein was immunoprecipitated with specific antisera. From this analysis the genes for NP, P/C, M, F, and HA were identified. In vitro translation of natural mRNAs and SP6 transcripts of cDNAs consistently produced smaller polypeptides that appear to be initiated at internal AUGs. The relative abundance of these various cell-free translation products reflects the probability of translational initiation at the various in-frame AUGs. The patterns observed suggest that other factors besides sequences immediately flanking the AUGs have a significant effect on the selection of translational initiation sites. An increase in translational efficiency of the F transcript was achieved by removing 450 bases of the G-C-rich 5’ noncoding r
ISSN:0300-5526
DOI:10.1159/000149994
出版商:S. Karger AG
年代:1987
数据来源: Karger
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5. |
Characterization of a Conformationally Sensitive Epitope on Moloney Murine Leukemia Virus gp70 |
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Intervirology,
Volume 28,
Issue 1,
1987,
Page 40-49
Richard Trauger,
Mark A. Rayfield,
Ronald B. Luftig,
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摘要:
We describe here several properties of a conformationally sensitive epitope common to the Moloney (M) and Rauscher (R) murine leukemia virus (MLV) gp70 family of glycoproteins, e.g., M-MLV gp70 and R-MLV gp71. This epitope was not detected by western blotting or by enzyme-linked immunosorbent assay experiments with three different lots of polyclonal R-MLV gp69/71 sera. However, it was detected when a specific monoclonal antibody, R47, was used in western blotting or immuno-dot-blotting experiments with the two viruses. R47 maps to a central 14-kd segment on R-MLV gp71 which spans an important structural domain, namely, that corresponding to the recombination site between ecotropic and endogenous envelope glycoprotein-coding sequences. Analysis of the western as well as dot blots developed with the R47 monoclonal antibody showed that about a 25-fold higher affinity for the epitope existed on R-MLV gp71, relative to M-MLV gp70. It thus appears that R-MLV and M-MLV gp70, although very closely related both structurally and serologically, are conformationally distinct in one domain, namely, the site of recombination between ecotropic and endogenous gp70-coding sequences.
ISSN:0300-5526
DOI:10.1159/000149995
出版商:S. Karger AG
年代:1987
数据来源: Karger
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6. |
Synthesis of Specific Transformation-Associated Proteins (TAPs) in Two Rat Cell Lines is Closely Related to the Expression of the v-mos Gene Product of Moloney Murine Sarcoma Virus (Mo-MSV) |
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Intervirology,
Volume 28,
Issue 1,
1987,
Page 50-56
Wanjun Li,
James M. Bowen,
James C. Chan,
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摘要:
The 6M2 cell line was established by transformation of normal rat kidney cells with the ts110 mutant of Moloney murine sarcoma virus (Mo-MSV). P85gag-mos was found to be the only known viral-transforming protein in this cell system. Previously, we described the detection in 6M2 cells of rat-specific transformation-associated proteins (TAPs) using a monoclonal antibody (MC). In this study, we used MC to investigate further the expression of TAPs at different temperatures and the time course of turn-on and shut-off of TAPs upon temperature shifting. It was found that TAPs were expressed at 24, 28 and 33 °C, but not at 37 and 39 °C. In experiments of temperature shifting, TAPs reappeared in about 6 h in 6M2 cells after the temperature was shifted from 39 to 33 °C, following closely the reappearance of p85gag-mos. The data in this communication support the notion that TAPs might be activated by the v-mos gene product during the process of transformati
ISSN:0300-5526
DOI:10.1159/000149996
出版商:S. Karger AG
年代:1987
数据来源: Karger
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7. |
Immunochemical and Biological Properties of a Mouse Monoclonal Antibody Reactive to Prunus Necrotic Ringspot Ilarvirus |
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Intervirology,
Volume 28,
Issue 1,
1987,
Page 57-64
J.A. Aebig,
R.L. Jordan,
R.H. Lawson,
H.T. Hsu,
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摘要:
A monoclonal antibody reacting with prunus necrotic ringspot ilarvirus was tested in immunochemical studies, neutralization of infectivity assays, and by immuno-electron microscopy. The antibody was able to detect the 27,000 Mr coat protein of prunus necrotic ringspot ilarvirus in western blots and also detected all polypeptide fragments generated after incubation of whole virus with proteolytic enzymes. In neutralization of infectivity studies, the antibody blocked virus infectivity, although it did not precipitate the antigen in agar gel Ouchterlony double diffusion tests. Immuno-electron microscopy confirmed that the antibody coats virions but does not cause clumping. The antibody may be a useful tool for investigating coat protein-dependent initiation of ilarvirus infection.
ISSN:0300-5526
DOI:10.1159/000149997
出版商:S. Karger AG
年代:1987
数据来源: Karger
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