摘要:

Two new strains of murine leukemia virus, one (strain 4996) purely ecotropic and the other (strain 1313) purely amphotropic, were isolated from spontaneous lymphomas in aged wild mice (Mus musculus). The 4996 virus is the first wild mouse field isolate which consists solely of ecotropic virus without the concomitant presence of amphotropic virus. The 1313 isolate is distinct in host range from seven other previously described wild mouse amphotropic isolates and is also the only murine leukemia virus shown to replicate in chicken cells.

ISSN:0300-5526    

DOI:10.1159/000148908

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

Hepatitis B core antigen (HBcAg) was purified from Dane particles and from infected hepatocytes. An identical isoelectric pH of 4.0 was determined for labeled preparations of both Dane-derived and liver-derived HBcAg. Unlabeled liver-derived HBcAg demonstrated a lower isoelectric pH of 3.7. Molecular weight determinations by Sepharose 4B column chromatography revealed that liver-derived HBcAg had a molecular weight of 8.5–9.0 × 106 daltons. The sedimentation coefficient of both Dane- and liver-derived HBcAg was found to be 124S. PAGE revealed that iodinated HBcAg derived from Dane particles was very similar in polypeptide structure to HBcAg derived from infected liver tissue. Twelve polypeptides were resolved from Dane core particles, and seven to nine were resolved from liver core particles. Several of the polypeptides in both preparations co-migrated with iodinated hepatitis B surface antigen (HBsAg). However, three polypeptides (mol. wt. 88,000, 79,000 and 59,000) were found in both Dane- and liver-derived HBcAg but not in HBsAg, which suggests that these polypeptides are HBcAg-specific. Endogenous DNA polymerase activity was observed in both Dane- and liver-derived core particl

ISSN:0300-5526    

DOI:10.1159/000148909

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

From skin papillomas of the chaffinch (Fringilla coelebs), a virus has been purified and studied by physicochemical techniques and electron microscopy. The virions measure 52 nm in diameter and are composed of 72 morphological units arranged in a skew T ≈ 7d surface lattice. A sedimentation coefficient of about 300S and a buoyant density of 1.34 g/ml in CsCl were determined for the particle. Its protein composition resembles that of human papillomavirus, and the circular double-stranded genome measures 2.6 µm. This is the first demonstration of a member of the Papovaviridae family that affects a non-mammalian ho

ISSN:0300-5526    

DOI:10.1159/000148910

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

A method for detecting Junin virus viremia in guinea pigs is described. The method consists of infecting BHK-21 cells with blood samples from infected guinea pigs; 48 h later, Junin virus antigens are detected in the cells by indirect immunofluorescence. Application of this technique to patients with Argentine hemorrhagic fever may lead to the quickest method for the virologic diagnosis of this disease.

ISSN:0300-5526    

DOI:10.1159/000148911

出版商:S. Karger AG    

年代:1977

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000148912

出版商:S. Karger AG    

年代:1977

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000148913

出版商:S. Karger AG    

年代:1977

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000148907

出版商:S. Karger AG    

年代:1977

数据来源: Karger