ISSN:0300-5526    

DOI:10.1159/000149465

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

The World Health Organization (WHO) convened a Scientific Group to adapt its program in virus diseases to recent progress in virology. The program consists of (a) general activities, such as reference services and the supplying of reagents by the WHO Collaborating Centres and (b) specific activities to solve problems – including the promotion of necessary research – caused by certain diseases of public health importance. The Group reviewed problems caused by influenza and other respiratory viruses, enteroviruses, gastroenteritis viruses (for which types A and B have been proposed as a convenient nomenclature), viral hepatitis, viruses in water and sewage, arboviruses, arenaviruses and Marburg virus, measles and rubella vaccination, smallpox, rabies, chronic infections, herpesviruses, oncogenic viruses, congenital infections, nosocomial infections, chlamydial and rickettsial infections, and mycoplasma infecti

ISSN:0300-5526    

DOI:10.1159/000149466

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

The transmission of Creutzfeldt-Jakob disease (CJD) to the patas monkey, Erythrocebus patas, has been accomplished. Clinical neurological disease was first noticed 47 months and 23 days after intracerebral inoculation of infected squirrel monkey brain. The disease progressed slowly and lasted 8 months. Microscopic examination of the brain and spinal cord revealed lesions of status spongiosus and astrogliosis in the cortical gray matter consistent with a diagnosis of experimental CJD. A vacuolating ‘spongiform’ cytopathological effect was seen in many of the in vitro cultivated brain cells in the outgrowth from explanted brain tissue of this mon

ISSN:0300-5526    

DOI:10.1159/000149467

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

A BALB/c continuous cell line, 238, was transformed following infection with UV-inactivated herpes simplex virus type 2 (HSV-2) and was designated H238. Several cell lines were derived from single cells of H238 by cloning in soft agar. The clonal lines express cytoplasmic antigens by immunofluorescence when reacted with rabbit HSV-2 antiserum and mouse tumor-bearer serum. The cloned cell lines induce tumors in immuno-competent mice by the subcutaneous route with a 100% incidence at an inoculum of 106 cells. These properties are consistent with those of the H238 parent cell line. In contrast to the parent line, however, the cloned cell lines do not elicit HSV neutralizing antibody in the tumor-bearing host.

ISSN:0300-5526    

DOI:10.1159/000149468

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

Granulins and polyhedrins from five baculoviruses exhibit similar chemical and physical properties. Although similarities are demonstrated, it has been shown that each of the proteins is different and apparently specific to a given virus. The granulins and polyhedrins exhibit a major polypeptide component of an estimated molecular weight of 28,000. Alkaline protease activity has been detected in each preparation. N-terminal analyses reflect differences between granulins and polyhedrins studied: granulosis virus = Asx, and nuclear polyhedrosis virus = Glx. Two-dimensional high-voltage electrophoresis of highly purified granulin and polyhedrin preparations reveals relatedness as well as differences among the proteins as assessed by electrophoretic migration patterns.

ISSN:0300-5526    

DOI:10.1159/000149469

出版商:S. Karger AG    

年代:1975

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000149470

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

Mixed infection of Vero cells with measles and vesicular stomatitis viruses gives rise to phenotypically mixed infectious virus particles possessing the properties of pseudotypes. Similar results were obtained when vesicular stomatitis virus was used to superinfect a defective measles system.

ISSN:0300-5526    

DOI:10.1159/000149471

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

Baby hamster kidney (BHK21) cells enucleated with cytochalasin B were infected with Pichinde virus or Sindbis virus. Viral replication was measured by plaque assay, and the synthesis of viral antigens was determined by immunofluorescence. Pichinde virus replication was completely inhibited in cells enucleated prior to infection as measured by both techniques while the replication of Sindbis virus was unaffected. Enucleation of cells at different times after infection with Pichinde virus indicated that intact nuclei were required for at least 8 h after infection.

ISSN:0300-5526    

DOI:10.1159/000149472

出版商:S. Karger AG    

年代:1975

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000149473

出版商:S. Karger AG    

年代:1975

数据来源: Karger