摘要:

Rat brain glioma cells were semipermissive for herpes simplex virus (HSV) replication, because the growth of HSV was multiplicity-dependent in these cells. By using this property, we successfully isolated ‘survivor’ glioma cells following HSV infection at low multiplicity and without using any special treatment (such as UV irradiation) either of the cells or of the virus. Under the same conditions there were no survivor BHK or 3T3 cells, which suggests the uniqueness of the glioma cell-HSV interaction. The survivor cells ceased to produce infectious virus after two subcultures, but were highly resistant to superinfection for at least 20 subcultures. Parental cells were significantly more permissive for homologous virus growth than survivor cells. Interferon was apparently not induced in the survivor cells, because they were as susceptible as the parental cells to infection with vesicular stomatitis virus. The survivor cells produced HSV-specific antigens and contained HSV-specific

ISSN:0300-5526    

DOI:10.1159/000149604

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Guinea pigs born from mothers infected before or during pregnancy with 103 PFU of the attenuated XJC13 or XJO strains of Junin virus (JV) by the intramuscular route showed 31.5% mortality that was not attributable to the mothers’ clinical condition or to lack of care. There was a slight drop in mortality rate when the mothers were infected at the beginning or end of their gestation period. JV isolation from the 9 offspring killed from 1 to 125 days of age proved that virus transmitted transplacentally or soon after birth was able to persist, although titers were not higher than 1027 PFU/g of tissue in various organs, including brain. Cell-associated viremia could thus account for viral spread after birth. Since an active humoral response was detected in the same animals, although Nt antibody titers were below 1:16, a state of tolerance did not exist in these congenitally infected animals. The carrier state appeared to modify guinea pig susceptibility to JV; after challenge with the pathogenic XJ strain of JV, 2 animals survived and developed normal humoral responses, while half of the remaining animals did not show typical signs of Argentine hemorrhagic fever. Although JV persistence appeared to cause no deleterious effects in surviving guinea pigs, its long-term risk remains to be determine

ISSN:0300-5526    

DOI:10.1159/000149605

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Arkansas bee virus (ABV) is a 30-nm isometric virus composed of one major species of polypeptide (mol. wt. 43 × 103) and one species of single-stranded RNA (mol. wt. 1.8 × 106). The size of the genomic RNA and the lack of evidence for encapsidation or synthesis of a second smaller RNA species exclude further consideration of this virus for inclusion with the physicochemically similar viruses of the Nodaviridae. Several independent isolations of ABV were made from bees, and in each case it was associated in mixed infection with a picorna-like virus termed Berkeley bee picornavirus (BBPV). No evidence for replicative dependence, serological relationship, or genome relatedness by complementary DNA hybridization was obtained between ABV and BBP

ISSN:0300-5526    

DOI:10.1159/000149606

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Infection of Acholeplasma laidlawii cells by the temperate enveloped mycoplasma virus L2 results in production of three morphological forms of progeny L2 virus: L2-I, L2-II, and L2-III. These morphological forms can be separated by velocity sedimentation and agarose gel electrophoresis. The latter technique was used to size the quasi-spherical particles: L2-I is 74 nm, L2-II is 88 nm, and L2-III is 132 nm in diameter. The protein composition of the three L2 forms is the same, although there are differences in protein stoichiometric ratios. L2-I, L2-II, and L2-III have the same 11.8 kilobase pair superhelical DNA genome. However, UV inactivation studies and restriction frequency measurements indicate that L2-I and L2-III each contain 1 genome copy, while L2-II contains 2–3 genome copie

ISSN:0300-5526    

DOI:10.1159/000149607

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

60 hexon-specific mouse hybridoma clones were selected by using crystallized hexon capsomers of human adenovirus type 1 as immunizing and selecting antigen. The reactivities of the monoclonal antibodies were tested with purified hexon preparations from 10 human adenovirus types by enzyme-linked immunosorbent assay and passive hemagglutination techniques. The reactivity patterns delineated a total of 41 adenovirus-type-1-related determinants present on a number of heterologous hexons in different interspecies combinations. The pattern of interspecies specificities did not coincide with the current grouping of adenoviruses into subgenera.

ISSN:0300-5526    

DOI:10.1159/000149608

出版商:S. Karger AG    

年代:1985

数据来源: Karger

摘要:

Groups of 6–39 monoclonal antibodies identifying 3–18 distinct epitopes on the nucleoprotein (NP), polymerase (P), hemagglutinin (H; equivalent in canine distemper and rinderpest viruses), and fusion (F) components of measles and canine distemper viruses were characterized in immunofluorescence tests with fixed Vero cell cultures infected with measles, canine distemper and rinderpest viruses. The majority of NP-specific monoclonal antibodies reacted with all three viruses, but one-third of the antibodies only reacted with the homologous virus. A few antibodies detected epitopes uniquely shared between either measles and rinderpest viruses or canine distemper and rinderpest viruses. Of the P-specific antibodies, two-thirds only reacted with the homologous virus, one antibody detected an epitope shared between canine distemper and rinderpest viruses, and the rest reacted with all three viruses. Also, the majority of antibodies against the H component were type-specific, but four antibodies reacted both with measles and rinderpest viruses. In contrast, the F component was antigenically highly conserved. 17 of 21 antibodies against this component reacted with all three viruses; one antibody reacted only with measles and rinderpest virus F components, and three antibodies reacted only with the homologous virus. No monoclonal antibody of any specificity selectively reacted with only measles and canine distemper viruses. Furthermore, the measles virus H component appeared to be more closely related to the equivalent rinderpest virus component than to the canine distemper virus component. Thus, it is proposed that rinderpest virus is the archevirus of the morbillivirus group from which canine distemper virus was first to evolve and, more recently (perhaps about 5,000 years ago), measles vi

ISSN:0300-5526    

DOI:10.1159/000149609

出版商:S. Karger AG    

年代:1985

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000149610

出版商:S. Karger AG    

年代:1985

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000149611

出版商:S. Karger AG    

年代:1985

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000149603

出版商:S. Karger AG    

年代:1985

数据来源: Karger