ISSN:0300-5526    

DOI:10.1159/000149666

出版商:S. Karger AG    

年代:1986

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000149667

出版商:S. Karger AG    

年代:1986

数据来源: Karger

摘要:

Herpes simplex virus type 1 (HSV-1) latency was established in human embryo lung (HEL) cells by treatment with (E)-5-(2’-bromovinyl)-2’-deoxyuridine and human leukocyte interferon and subsequent temperature increase (from 37° to 40.5°). This in vitro system was used to study reactivation after temperature shift-down to 37°. Four methods known to induce cell DNA synthesis, superinfection with human cytomegalovirus, increased serum concentration in the medium, wound production, and superinfection with simian virus 40 (SV40), accelerated activation and replication of HSV-1. In contrast, reduced serum concentration resulted in postponement of virus reactivation and lower yield. In control experiments with mock-infected HEL cells treated with inhibitors and maintained for prolonged periods of time at 40.5°, [3H]-thymidine incorporation was stimulated by increased serum concentration in the medium, wounding and SV40 superinfection, and hampered by reduced serum concentration in the medium. The data seem to indicate that cell DNA synthesis, the processes associated with it, or both, play a key role in the activation of latent HSV-1 and subsequent virus replication in this

ISSN:0300-5526    

DOI:10.1159/000149668

出版商:S. Karger AG    

年代:1986

数据来源: Karger

摘要:

Biopsies from human genital lesions in Japan (108 samples), including condyloma acuminata, squamous metaplasia, dysplasia, and cervical cancer, were screened for the presence of human papillomavirus (HPV) 6-, 11-, 16-, and 18-related DNAs by spot hybridization and Southern blot hybridization under stringent conditions. By spot hybridization, HPV 6/11-related DNA was found in 92.9% (13/14) of condyloma acuminata and in 6.7% (1/15) of cervical cancer biopsies. HPV 16/18-related DNA was found in 37.7% (5/14) of cervical cancer biopsies and was exclusively associated with invasive squamous cell cancer, 66.7% (8/12), and with cervical carcinoma in situ, 50% (9/18). Some sample DNAs were further characterized by restriction enzyme digestion followed by Southern blot analysis, and we confirmed the presence of HPV-specific DNA fragments and mixed infection with both HPV 6/11- and HPV 16/18-related HPVs in one lesion.

ISSN:0300-5526    

DOI:10.1159/000149669

出版商:S. Karger AG    

年代:1986

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000149670

出版商:S. Karger AG    

年代:1986

数据来源: Karger

摘要:

With the objective of generating DNA molecules that form stable stem-loop structures or cruciform structures in solution, we have altered the palindromic sequence at the Simian Virus 40 (SV40) replication origin. These alterations include: (i) deletion of 18 of the 27 base pairs (bp) in the 13-bp inverted repeat; (ii) deletion of 26 of the 27 bp; (iii) substitution of the entire 27 bp with a totally different 26-bp sequence containing a 13-bp inverted repeat; and (iv) substitution of the 27 bp with an 8-bp sequence containing a 4-bp inverted repeat. The DNA from these mutants was purified. Mutant DNAs were hybridized to wild-type SV40 DNA or to each other, and the heteroduplexes were purified. The predicted structures were verified by S] and restriction endonuclease digestion. The mutants – the heteroduplexes have not been tested – are capable of complementing tsA mutants and/or transforming mouse ce

ISSN:0300-5526    

DOI:10.1159/000149671

出版商:S. Karger AG    

年代:1986

数据来源: Karger

摘要:

The restriction endonuclease patterns of three Baculovirus isolates (Br, Vh, El) from Heliothis zea having different passages and production histories were compared. Digestion of the ds DNA genomes of the three isolates with EcoRl, Hindlll, and Xhol showed no major difference in the cleavage patterns, although submolar fragments were detected. One of the commercial isolates (Vh) was serially passaged 20 times through larvae of H. zea and a substitute host, H. virescens. EcoRl cleavage profiles of the ds DNA showed the absence of a band in the 3-megadalton region that was present in the original isolates. In addition, the cleavage pattern of the DNA from Vh passed in H. zea showed additional submolar fragments in the 15- and 6-megadalton regions. Differences also were observed in the Hindlll and XhoIrestriction patterns. No significant differences in virulence between the original isolate and passaged isolates were detected after 20 serial passages in larvae of either H. zea or H. virescens.

ISSN:0300-5526    

DOI:10.1159/000149672

出版商:S. Karger AG    

年代:1986

数据来源: Karger