摘要:

The immunogenic capsid protein (VPt), circa 30 kilodaltons (kd), of foot-and-mouth disease virus was examined for (i) its ability to induce neutralizing antibody in guinea pigs after chemical modifications and CNBr or tryptic cleavages and (ii) N-terminal amino acid sequence homology across three virus types. The immunogenicity of VPt was inactivated by glutaraldehyde treatment, carboxymethylation and maleylation or citraconylation. However, de-citraconylation restored part of the lost activity. Cleavage of type A12 VPt with CNBr produced an immunogenic peptide of circa 13 kd. A slightly larger (ca. 16 kd) immunogenic doublet, VPτa¾, was obtained by tryptic cleavage of VPt in the virion. Sequence homologies of circa 85% were found between the first 26 amino acids at the N-terminus of VP chains from virus types A12 strain 119 (A12), C3 Resende (C311) and Oi Brugge (Oib

ISSN:0300-5526    

DOI:10.1159/000149070

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

A comparative biochemical study of virus-induced DNA polymerases was made among the herpes group viruses: namely, herpes simplex virus (HSV) type 1 and type 2, human cytomegalovirus (HCMV) and varicella-zoster virus (VZV). Although these virus-induced enzymes shared some biochemical properties, they differed in several important aspects. All these virus-induced DNA polymerases could efficiently use poly(dC) · oligo(dG)i2-i8 and poly(dA) · oligo(dT)i2-i8 as template-primers. However, in phosphocellulose chromatography, HSV-1-and HSV-2-induced enzymes were eluted at the low concentration of 0.18–0.20 M NaCl and the counterparts of HCMV and VZV were eluted at 0.30–0.32 M. The former two enzymes were more sensitive to lower concentrations of phosphonoacetate and ethyl phosphonoacetate than the latter two enzymes. Moreover, the activity of HSV-1- and HSV-2-specified DNA polymerases was 5 times greater in the presence of 60 mM ammonium sulfate if poly(dA) · oligo(dT)i2-i8 was used as template-primer, while HCMV- and VZV-induced enzyme activities were only about twice as great under the same conditions. Furthermore, DNase activity was conspicuous in both HSV-1- and HSV-2-infected WI-38 cells, but was not detectable in HCMV-and VZV-infected cells. After storage for 1 year at 4°, the HSV-1-induced DNA polymerase was the most thermostable of the four viral

ISSN:0300-5526    

DOI:10.1159/000149071

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Cytomegalovirus was isolated from cell cultures derived from 3 of 16 surgical specimens of adenocarcinomas of the colon. Virus identification was accomplished through electron microscopical, cytochemical, and immunofluorescent procedures.

ISSN:0300-5526    

DOI:10.1159/000149072

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Virus could be rescued by cell fusion techniques from the H50, BRKSV (Bam I linear), 14B and 14B (1–4) cell lines, which contain the entire SV40 genome but have previously yielded negative results by this method. Virus could not be rescued from the T22 cell line, which was transformed by defective virus. The T-antigen-negative flat revertant cell line, 14B (1–4), converted to T-antigen-positive prior to rescue. Subculture and extensive monitoring for production of infectious virus appeared to be prerequisite for detecting virus res

ISSN:0300-5526    

DOI:10.1159/000149073

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Measles virions and cytoplasmic nucleocapsids were labeled with [3H]-amino acids and [32P]-orthophosphate and were purified from infected Vero cells. When analyzed by PAGE, the two capsid-associated polypeptides (VP2 – 69,000 daltons; VP3 – 60,000 daltons) were shown to be phosphorylated. Characterization of the phosphorylated polypeptides by acid hydrolysis and high-voltage paper electrophoresis showed that serine was the major phosphorylated amino acid, although lesser amounts of phosphothreonine were also present. The possible role of phosphorylation in the replication cycle of the virus is discus

ISSN:0300-5526    

DOI:10.1159/000149074

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Human embryo fibroblasts treated with diethylstilbestrol (DES) gave rise to slightly increased plaque production by cytomegalovirus (CMV); plaques were also slightly larger in treated cells when compared to those in untreated cultures. There was no significant enhancement of plaque production by herpes simplex virus (HSV) types 1 and 2 in the human cells or in primary rabbit kidney cells treated with DES. Growth analyses of HSV and CMV in DES-treated cells failed to reveal enhancement of virus production when compared to untreated cells. The frequency of biochemical transformation of mouse cells deficient in thymidine kinase (TK) to the TK+ phenotype by HSV was markedly enhanced when cells were pretreated with DES. TK+-transformed colonies arising from DES-treated cells were also larger than those derived from untreated cultures. These data reveal that DES increases susceptibility of human cell lines to CMV infection and enhances efficiency of biochemical transformation of mouse cells by HSV.

ISSN:0300-5526    

DOI:10.1159/000149075

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Five epithelioid cell lines were established from invasive cervical carcinoma tissue and were examined for herpes simplex virus (HSV) antigens or virus-induced changes. Immuno-fluorescence studies with antiserum to an HSV type 2 nonstructural polypeptide detected peri-nuclear antigens. In these cell lines, concentrations of a membrane fucopeptide that is regularly present in normal cells were reduced to levels similar to those previously observed in virus-transformed cells. One of the cell lines inoculated into athymic mice produced tumors.

ISSN:0300-5526    

DOI:10.1159/000149076

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

A micro solid phase radioimmunometric assay has been developed which measures type-specific antibody in human sera to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The test is performed by coating wells of microtiter plates with purified HSV-specified glycoproteins, VP123 and VP119, derived from HSV-1- and HSV-2-infected cells, respectively. The coated wells are then incubated with diluted unadsorbed sera and subsequently with radio-iodinated goat anti-human IgG reagent. Because these major viral envelope glycoproteins contain type-specific antigenic determinants, type-specific antibodies are preferentially detected in our assay.

ISSN:0300-5526    

DOI:10.1159/000149077

出版商:S. Karger AG    

年代:1979

数据来源: Karger

摘要:

Xenotropic murine type-C virus was induced after X-ray or UV light irradiation of K-Balb cells. The induction was markedly suppressed by postirradiation treatment of the cells with antipain, a protease inhibitor of microbial origin. Virus expression was unaffected when the cells were treated with halogenated pyrimidines.

ISSN:0300-5526    

DOI:10.1159/000149078

出版商:S. Karger AG    

年代:1979

数据来源: Karger

ISSN:0300-5526    

DOI:10.1159/000149079

出版商:S. Karger AG    

年代:1979

数据来源: Karger