| 31. |
Call forConcepts and InnovationsContributions |
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Neurosurgery,
Volume 45,
Issue 4,
1999,
Page 944-944
&NA;,
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ISSN:0148-396X
出版商:OVID
年代:1999
数据来源: OVID
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| 32. |
Announcements |
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Neurosurgery,
Volume 45,
Issue 4,
1999,
Page 949-949
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ISSN:0148-396X
出版商:OVID
年代:1999
数据来源: OVID
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| 33. |
Preservation of the Olfactory Tract in Bifrontal Craniotomy for Various Lesions of the Anterior Cranial Fossa |
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Neurosurgery,
Volume 45,
Issue 4,
1999,
Page 955-955
Abolghassem,
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ISSN:0148-396X
出版商:OVID
年代:1999
数据来源: OVID
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| 34. |
Emergency Magnetic Resonance Imaging of Cervical Spinal Cord Injuries: Clinical Correlation and Prognosis |
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Neurosurgery,
Volume 45,
Issue 4,
1999,
Page 956-956
Douglas,
Quint Nathan,
Selden Stephen,
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ISSN:0148-396X
出版商:OVID
年代:1999
数据来源: OVID
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| 35. |
Endoscopic Third Ventriculostomy: Outcome Analysis of 100 Consecutive Procedures |
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Neurosurgery,
Volume 45,
Issue 4,
1999,
Page 957-957
Nikolai,
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ISSN:0148-396X
出版商:OVID
年代:1999
数据来源: OVID
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| 36. |
Treatment of an Intracranial Aneurysm Using a New Three-Dimensional-Shape Guglielmi Detachable Coil: Technical Case Report |
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Neurosurgery,
Volume 45,
Issue 4,
1999,
Page 959-959
Adel,
Malek Randall,
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ISSN:0148-396X
出版商:OVID
年代:1999
数据来源: OVID
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| 37. |
With Appreciation |
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Neurosurgery,
Volume 45,
Issue 4,
1999,
Page 961-961
&NA;,
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ISSN:0148-396X
出版商:OVID
年代:1999
数据来源: OVID
|
| 38. |
Retroviral Vector-Mediated Transfer and Expression of Human Tissue Plasminogen Activator cDNA in Bovine Brain Endothelial Cells |
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Neurosurgery,
Volume 45,
Issue 4,
1999,
Page 962-962
Hong Yu,
Ying Wang,
Darwin Eton,
Monique Stins,
Liang Wang,
Michael Apuzzo,
Fred Weaver,
J. McComb,
Martin Weiss,
Berislav Zlokovic,
&NA; &NA;,
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摘要:
OBJECTIVESGene transfer of thrombolytic enzymes to vascular endothelial cells may influence the kinetics of intravascular thrombosis. This study defines the potential for gene transfer of tissue plasminogen activator (tPA) into bovine brain endothelial cells (BBEC).METHODSThe retroviral vectors derived from murine leukemia virus (MuLV) were used to transfer human tPA cDNA to BBEC. The tPA activity, tPA antigen and tPA inhibitor 1 (PAI-1) antigen were determined in the supernatant of transduced (BBEC/tPA) cell cultures by an immunoassay.RESULTSThe tPA antigen and enzymatic activity in cell culture supernatants of BBEC/tPA transduced cells were 75 ng/ml and 14 IU/ml after 4 days, that was 25 and 28-fold higher compared to the respective values in control cells. The PAI-1 antigen was not affected by tPA cDNA transfer. The Western blot assay of cell lysates confirmed that the majority of tPA in BBEC/tPA transduced cells was in the form of free tPA. While the maximal transduction efficiency of BBEC with an amphotropic MuLV vector was about 15%, a MuLV pseudotyped with vesicular stomatitis virus G glycoprotein envelope achieved high > 90% maximal transduction efficiency.CONCLUSIONSThe fibrinolytic activity of brain endothelial cells can be enhanced by transferring human tPA cDNA. These findings provide an initial step in implementation of future studies that investigate the use of this technology as an adjunctive treatment for cerebrovascular disease.
ISSN:0148-396X
出版商:OVID
年代:1999
数据来源: OVID
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| 39. |
Departments: Calendar of Events |
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Neurosurgery,
Volume 45,
Issue 4,
1999,
Page 971-971
&NA;,
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ISSN:0148-396X
出版商:OVID
年代:1999
数据来源: OVID
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| 40. |
Your Help to Preserve Neurosurgical History |
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Neurosurgery,
Volume 45,
Issue 4,
1999,
Page 974-974
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ISSN:0148-396X
出版商:OVID
年代:1999
数据来源: OVID
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