摘要:

Summary:We examined 1,541 consecutive serum samples from 707 children with suspected food intolerance and 32 with treated celiac disease (CD) for IgG and IgA antibody reactivities to antigens from gluten, egg, and cow's milk by an enzyme-linked immunosorbent assay (ELISA). Samples from 72 patients showed increased IgA and/or IgG reactivity to gluten antigens; four were known CD patients not complying with a gluten-free diet, 13 were suspected CD patients challenged with gluten, and 30 most likely had CD as suggested by small intestinal villous atrophy and histological and/or clinical improvement on a gluten-free diet. The remainder with increased antigluten activity had other disorders that might have affected mucosal permeability. Nevertheless, the median IgA reactivity to gluten was significantly higher in the CD group, and the probability for CD increased from 25 to 100% when this reactivity was above 2.4 optical density (OD) units in our ELISA. Sixteen CD patients (but none of those without CD) had IgA reactivity to gluten higher than 2.4 OD units. We conclude that ELISA determinations of levels of serum antibodies reacting to dietary antigens is a valuable adjunct in the diagnosis of CD in children.

ISSN:0277-2116    

出版商:OVID    

年代:1990

数据来源: OVID

摘要:

Summary:Conflicting reports in the literature regarding the sensitivity and specificity of the acetylcholinesterase (AChE) stain in establishing or excluding the diagnosis of Hirschsprung's disease (HD) prompted this review of 497 rectal biopsies performed on 455 children. Using hematoxylin and eosin (H&E) to stain formalin-fixed, paraffin-embedded tissue sections is our preferred method of identifying ganglion cells. In this series, however, there were eight children with HD, and nine without HD in whom the AChE-stained portion of the sample provided invaluable diagnostic information not obtained from the concomitant, formalin-fixed, H&E-stained portion of the sample. The AChE stain also provided at least suggestive evidence of HD in some of the anal or anorectal biopsy specimens.

ISSN:0277-2116    

出版商:OVID    

年代:1990

数据来源: OVID

摘要:

Summary:Protein (P), fat (F), and carbohydrate (C) concentration in expressed human bank milk was determined by infrared analysis of 2,554 samples from 224 mothers. The mean contents of P, F, C, and energy (E, calculated from P, F, and C) were 9.0 g/L, 39.0 g/L, 71.9 g/L, and 696 kcal/L, respectively. There was a large variation in the concentration of energy-yielding macronutrients. The contents of P. F, C, and E in the samples with the highest values (97.5 percentile) were 2.3-, 4.8-, 1.2-, and 2.3-fold, respectively, above the contents in the samples with the lowest values (2.5 percentile). The P content decreased exponentially during the 1st 8 months, followed by an increase during the following months. The F content decreased during the 1st 4 months, followed by an almost linear increase. The possible influence of different maternal characteristics on the macronutrient content of the milk was examined. The main results were as follows: the P and F contents increased slightly with increasing body mass index of the mother, the P content decreased with increasing amounts of milk delivered to the milk bank, and the F content was higher in mothers delivering large amounts of milk. By selecting incoming milk with a high P content, we have developed a “high-protein” milk with a P content of about 12 g/L (true protein) and an E content of about 725 kcal/L. Thus, by continuous monitoring of macronutrient content in human bank milk it is possible to develop a “high-protein” milk with sufficient P and E content to cover the needs of preterm infants with very low birth weights (< 1,500 g).

ISSN:0277-2116    

出版商:OVID    

年代:1990

数据来源: OVID

摘要:

Summary:An infant formula based on high protein rice flour, with added lysine and threonine, was evaluated in recovering malnourished infants. Acceptability, tolerance, and the digestibility of most major nutrients (energy 93.9 ± 0.7%, fat 96.9 ± 0.3%, carbohydrate 96.8 ± 0.6% of intake) were excellent. Digestibility of protein, as with all rice products, was moderately low (80.1 ± 3.3%), but its high biological value made N retention equal to that from casein. Plasma-free amino acids were consistent with the low essential/total amino acids ratio found in cereals, with slower absorption, and with the possibility that leucine was the first-limiting amino acid. Absorptions of Ca, Mg, PO4, and Zn were considered satisfactory. Energy costs of weight gain and the estimated percentage N in the weight gained during 14 days were comparable to those attained with the highest quality cow's milk-derived formulas in children of similar ages and nutritional status.

ISSN:0277-2116    

出版商:OVID    

年代:1990

数据来源: OVID

摘要:

Summary:Oxygen-derived free radicals are an important component of gastrointestinal injury in necrotizing en-terocolitis (NEC). To assess the effect of a 72-h fast on the ability of neonatal small bowel to metabolize free radicals, the activity of superoxide dismutase (SOD), catalase, and the glutathione cycle were quantitated in mucosal scrapings from proximal and distal small bowel of fed and fasted neonatal rabbits. Hexose monophosphate shunt activity, quantitated in enterocytes from fed and fasted neonatal rabbits, was significantly less, p < 0.01, in fasted animals. SOD activity was lower in distal small bowel from fasted animals than fed. The two mechanisms available to metabolize H2O2, catalase and the glutathione cycle, were significantly lower in both proximal and distal small bowel from fasted animals than in those from fed animals. To determine if fasting caused decreased enzyme activity at the level of gene expression, gastrointestinal tract DNA, RNA and protein, and specific mRNA levels for catalase, glutathione peroxidase (GPx), and SOD were quantitated. DNA, total RNA, and mRNA for SOD were lower in mucosal scrapings from fasted animals. However, mRNA for catalase, and GPx were not lower in fasted animals. Thus, a 72-h fast in neonatal rabbits causes a regional-specific decrease in SOD activity, which may be explained by decreased transcription. Changes in transcription do not completely account for regulation of catalase and glutathione cycle enzymes.

ISSN:0277-2116    

出版商:OVID    

年代:1990

数据来源: OVID

摘要:

Summary:The movement of water and transport of Na+and Cl by mid-small intestine (M-SI) of rotavirus-infected neonatal mice was investigated by an in vitro perfusion technique. The concentrations of Na+, K+, and Cl in the luminal contents of upper, middle, and lower small intestine and colon of infected mice were determined by flame photometry (Na+, K+) and an ion selective microelectrode (Cl). In M-SI, maximal disturbance of water transport occurred at 72 h postinfection (PI): Infected tissue exhibited net water secretion. Water transport was also impaired at 144 h PI. Net secretion of Cl occurred at 72 h PI, with some evidence of a second phase of reduced magnitude at 120–144 h PI. The magnitude and statistical significance of changes in Na+transport were both less than those for Cl, but the pattern of change was similar to that for Cl. Luminal concentrations of Na+were elevated between 48 and 144 h PI in the small intestine; this was particularly so in distal regions. Luminal Cl concentration was maximally elevated to a considerable degree at 72 h PI and remained high at 96 h PI throughout the small intestine; thereafter, Cl concentration returned to near normal. K+concentration was unchanged in the small intestinal lumen; in the colon, however, K+concentrations were depressed 72–168 h PI. In the light of previous data from this laboratory, the present data are interpreted as evidence for a secretory component in rotavirus-induced diarrhea.

ISSN:0277-2116    

出版商:OVID    

年代:1990

数据来源: OVID

摘要:

Summary:Vitamin E deficiency is a common consequence of chronic cholestatic liver disorders. Inasmuch as vitamin E content of cellular membranes alters membrane properties such as fluidity and molecular order, we postulated that vitamin E status could affect hepatocyte transport processes dependent on membrane integrity. Hepatocytes were isolated from rats maintained on diets containing deficient, sufficient, or excess vitamin E. Cell viability and oxygen consumption were maintained in all groups of hepatocytes. Hepatocyte uptake of taurocholic acid and ouabain and Na+, K+-ATPase activity estimated by rubidium-86 influx did not differ with vitamin E status. Vitamin-E-deficient hepatocytes had increased generation of lipid peroxide products. We conclude that deficient or excess vitamin E status had little effect on selected transport processes in normal hepatocytes.

ISSN:0277-2116    

出版商:OVID    

年代:1990

数据来源: OVID

摘要:

Summary:A 4-year-old boy and a 14-year-old girl referred for a cholestatic syndrome were found to have a unifocal stricture of the extrahepatic bile duet. Both radiological (endoseopic-retrograde-choledocopancreatography) and histological findings were suggestive of primary sclerosing cholangitis. There was no past history of chronic inflammatory bowel disease nor any other disorder. Serum autoimmune markers were negative. Complete remission was observed after surgical drainage, and both patients remained symptom-free after a follow-up of 12 months and 8 years, respectively. Such unifocal form of the disease may have a better prognosis than the plurifocal disease. The absence of associated disorders and autoimmunity may also be an indicator of better prognosis in these patients.

ISSN:0277-2116    

出版商:OVID    

年代:1990

数据来源: OVID

摘要:

Summary:Heterotopic gastric mucosa (HGM) may be found anywhere in the gastrointestinal tract, most often in a Meckel's diverticulum. Approximately 30 cases of HGM, located in the small bowel beyond the ligament of Treitz and not associated with a Meckel's diverticulum, have been reported. They were most often revealed by intestinal intussusception, occasionally by perforation of an intestinal ulcer or intestinal bleeding. We report a 4-year-old boy who had three attacks of acute intestinal intussusception over a 5-month period resulting in surgery. Both physical examination and barium examination of the small bowel and large intestine were found to be normal between attacks. Peroperative palpation of the small bowel was normal during the three laparotomies. During the third operation, he underwent an intraoperative endoscopy (IOE), which revealed a polypoid mass 2 cm in diameter and 0.5 cm in height, 40 cm proximal to the ileocaecal valve. Histologic examination showed HGM with fundic glands, and chief and parietal cells. This case emphasizes the interest of IOE, the main indications of which are the localization of unknown sites of gastrointestinal bleeding and the search for hamartomatous polyps of the Peutz-Jeghers syndrome for polypectomy and/or segmental resection.

ISSN:0277-2116    

出版商:OVID    

年代:1990

数据来源: OVID

ISSN:0277-2116    

出版商:OVID    

年代:1990

数据来源: OVID