ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1991.tb03201.x

出版商:Blackwell Science Ltd    

年代:1991

数据来源: WILEY

摘要:

Three third‐generation white cell (WBC)‐depletion filters based on polyester layers with decreasing pore size were investigated. In the coarse layers, unaggregated granulocytes, monocytes, and platelets and aggregates of these cells were captured in close contact with the fibers. This indicates that the depletion of granulocytes, monocytes, and platelets in the coarse layers of the filters is due in part to activation and adhesion with the formation of cell clusters on the fibers. In Filter I and Filter II platelets were not found in the fine layers, whereas in Filter III, 70 percent of the platelets were detected as unaggregated platelets on the fibers of the fine layers. More than 95 percent of the lymphocytes captured in the three filters occurred as single cells in the fine layers, and over 60 percent of these could be recovered. This suggests that the depletion of lymphocytes depended on trapping of the cells in the fiber network. All three filters captured HLA‐DR‐positive lymphocytes in the top layers, a finding that supports earlier reports that the transfusion of filtered red cell concentrates reduces HLA alloimmunization. More lymphocytes and granulocytes were found in the last layer of Filters II and III than of Filter I. Therefore, the risk of white cell leakage is probably lowest for Filter I. Red cells were found as red cell aggregates in the fine layers of Filters I and II, whereas almost no red cells were detected in Filter III. It was shown that the three filters studied were similar in their removal of WBCs, but differed in their mechanisms of

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1991.31191096184.x

出版商:Blackwell Science Ltd    

年代:1991

数据来源: WILEY

摘要:

Studies of 91 individuals in three families allowed a genetic‐linkage analysis of the gene governing the production of the low‐incidence red cell antigen Wra and provided evidence that Wra is not a member of the Scianna, Landsteiner‐Wiener, Chido/Rodgers, or XK blood group systems, and that the “WR” locus is excluded from autosomal sites or regions 1p34‐p22.1, 1p21‐q23, 1q32, 2p25, 3q21, 4q28‐q32, 6p24‐q12, 9q34.1‐ q34.2, 13q14.1‐q14.2, 14q24.3‐q32.1, 14q32.33, 16p13, 16q22.1, and 21q21‐q22.1. “WR” is also excluded from within specified genetic distances of chromosomes 8 (GPT), 18 (JK), 19 (C3), 20 (ADA), and 22 (P1) loci, which brings its exclusion to approximately 10 percent (320cM) of the total genetic map of the genome. The possibility that “WR” is pseudoautosoma

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1991.31191096185.x

出版商:Blackwell Science Ltd    

年代:1991

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1991.tb03204.x

出版商:Blackwell Science Ltd    

年代:1991

数据来源: WILEY

摘要:

Pediatric blood transfusion practice in a tertiary‐care pediatric hospital was evaluated retrospectively by using the technique of criteria mapping. A total of 630 transfusion episodes involving red cell concentrates, frozen plasma (plasma frozen within 24 hours of collection), platelet concentrates, and albumin were reviewed: 243 (86.2%) were reviewed only by a technical assistant, and 87 (13.8%) required additional physician review. Of these, 138 were red cell concentrate transfusions: 79.7 percent of that group were considered appropriate, 11.6 percent of unknown benefit/risk ratio, 5.8 percent inappropriate, and 2.9 percent impossible to evaluate. Some 246 frozen plasma transfusions were reviewed: 42.3 percent were considered appropriate, 32.5 percent of unknown benefit/risk ratio, 17.5 percent inappropriate, and 7.7 percent impossible to evaluate. A total of 139 platelet concentrate transfusions were reviewed: 64.7 percent were considered appropriate, 16.5 percent of unknown benefit/risk ratio, 10.1 percent inappropriate, and 8.6 percent impossible to evaluate. Some 107 albumin transfusions were reviewed: 90.6 percent were considered appropriate, 1.9 percent inappropriate, and 7.5 percent impossible to evaluate. The criteria maps developed for this study were easy for the technical assistant to use, and areas of appropriate and inappropriate pediatric transfusion practice were clearly identifie

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1991.31191096186.x

出版商:Blackwell Science Ltd    

年代:1991

数据来源: WILEY

摘要:

Five women in the second or early third trimester of pregnancy underwent prophylactic partial red cell exchange by automated erythrocytapheresis for sickle cell disease. The purpose of this pilot study was to characterize the hematologic, hemodynamic, and metabolic changes associated with this procedure. Pretransfusion hematocrit increased 49 percent by 3 to 5 days after the transfusion. Paired observations indicate a 14‐percent drop in maternal oxygen consumption. There was a slight tendency for reduced posttransfusion cardiac output that resulted primarily from changes in maternal heart rate and not from changes in stroke volume. Left ventricular contractility also tended to decrease after transfusion. Neither fetal bradycardia nor alteration in the umbilical circulation by Doppler ultrasound was observed during the transfusion procedures. Despite substantial hematologic alterations, this procedure is associated with small or negligible changes in maternal hemodynamic and metabolic function for minimally symptomatic pregnant patients with sickle cell diseas

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1991.31191096187.x

出版商:Blackwell Science Ltd    

年代:1991

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1991.tb03207.x

出版商:Blackwell Science Ltd    

年代:1991

数据来源: WILEY

摘要:

A family is described in which the mother made anti‐Rh32 as a result of pregnancy; her second liveborn child had hemolytic disease of the newborn and required an exchange transfusion. In investigating the family, it was found that the father's Rn gene did not make rhi and that his second Rn gene made normal amounts of c and e but a reduced amount of f. In the two children of the couple, who inherited a normal r or Ro from their mother, the paternally derived RN encoded an amount of rhi that could be detected in direct typing tests. In the father, lack of production of rhi by RN may have represented a suppressive effect of the ce(f) gene in trans to Rn or the presence of an unlinked suppressor of Rh that might also have been responsible for the reduced production of f by his r or Ro gene. The two children in this family are the first persons in whom RN has been shown to make rh

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1991.31191096188.x

出版商:Blackwell Science Ltd    

年代:1991

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1991.31191096189.x

出版商:Blackwell Science Ltd    

年代:1991

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1991.31191096190.x

出版商:Blackwell Science Ltd    

年代:1991

数据来源: WILEY