摘要:

Newly prepared platelet concentrates (PC) and platelet poor plasma (PPP) stored at either room temperature (RT) or 4 C were analyzed for ammonia content initially, and after storage at 24, 48, and 72 hours. These studies demonstrate that ammonia rapidly accumulates in PC stored at RT. This accumulation appears to be temperature dependent, as evidenced by the observed differences in ammonia accumulation in PC stored at 4 C and RT. Whether ammonia acts as a toxic metabolite in the inhibition of platelet function and the development of the “storage lesion,” which occurs in PC stored at RT, needs further clarification. Additional studies are also necessary to establish the clinical efficacy of transfusing ammonia‐containing platelet concentrates into premature neonates with immature functioning livers, and perhaps into adults with severe liver di

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1981.tb05673.x

出版商:Blackwell Science Ltd    

年代:1981

数据来源: WILEY

摘要:

The effects on platelet function of intermittent‐flow centrifugation pheresis were measured employing platelets collected from ten donors by combined platelet‐leukapheresis with hydroxyethyl starch (LP) and from ten by plateletpheresis (PP) by similar techniques except without starch. Greater numbers of platelets were produced by LP than by PP. Aggregation of platelets collected by both LP and PP was normal (did not differ from prepheresis baseline) to collagen and to 10−5M adenosine diphosphate (ADP). Slight impairment to aggregation with 2 × 10−6M ADP and 5.5 × 10−5M epinephrine occurred with both techniques. These abnormalities, however, were significantly less severe in platelets collected by LP. Platelet morphology by electron microscopy was nearly normal, although glycogen granules were absent in LP platelets. Thus, hydroxyethyl starch, at doses currently used during a single LP, does not enhance abnormalities of platelet aggregation over those expected to result from PP alone. Actually, LP platelets function betterin vitrothan those collected by PP. Large numbers of platelets can be harvested by LP, and their use as a component that is comparable to PP platelets may mean improved efficiency and reduced costs for phere

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1981.tb05674.x

出版商:Blackwell Science Ltd    

年代:1981

数据来源: WILEY

摘要:

The preparation of leukocyte‐poor cell masses (LPCM) by saline washing with the IBM 2291 Blood Cell Processor was investigated. Units to be washed were preferentially selected from short‐dated units to reduce outdating. LPCM had a mean of 0.34 × 109WBC (90% WBC removal) with 87.8 per cent RBC recovery. At least 50 per cent of the LPCM prepared used blood that was salvaged from typically discarded units. Febrile transfusion reactions were prevented in alloimmunized patients with this product. Cell washing with the IBM Blood Cell Processor can prepare LPCM from blood of any storage date to meet American Association of Blood Banks (AABB) stand

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1981.tb05675.x

出版商:Blackwell Science Ltd    

年代:1981

数据来源: WILEY

摘要:

Two current problems in blood transfusion services are the widespread lack of information on this subject among practicing physicians, house staff, and medical students, and the dearth of broadly trained, full‐ time professionals in the field. Our most important and urgent responsibility is to train physicians who seek full‐time careers in any aspect of blood transfusion services, blood center management, hospital transfusion services, research, or combination thereof. Successful training programs require sufficient space, personnel, and funds. In addition, blood centers have a responsibility to educate practicing physicians and house staff by formal teaching sessions, or informally when problems arise. Medical school curricula usually contain little on blood banking; exposure to some basic immunohematology or a visit to a blood center will help sensitize students to the availability of blood. Hospital administrators, regional medical society officers, and corporate medical directors, informed of the blood centers'activities, can help improve relationships between center, hospital, and commun

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1981.tb05676.x

出版商:Blackwell Science Ltd    

年代:1981

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1981.tb05678.x

出版商:Blackwell Science Ltd    

年代:1981

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1981.tb05680.x

出版商:Blackwell Science Ltd    

年代:1981

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1981.tb05681.x

出版商:Blackwell Science Ltd    

年代:1981

数据来源: WILEY