ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17377196349.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

A rapid, fast, and accurate method of determining bacterial contamination in blood units is needed to provide a safe unit of frozen‐ reconstituted blood which can be stored at 4 C after thawing for more than the 24 hours currently allowed by law. The Bactec instrument seems to provide this method. It detects most of the organisms which have been reported in contamineted blood, and does this in a short enough period of time that any organisms introduced into the blood unit during the sampling process will not grow sufficiently to contaminate the unit while the culturing process is going o

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17377196350.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

The survival in vivo of 51Cr‐labeled autologous human platelets, the serotonin uptake velocity, and the response to hypotonic stress were determined both before and after cryopreservation. Changes in platelet survival corresponded to changes in serotonin uptake velocity and response to hypotonic stress. No correlation was observed between the values of the survivla in vivo, the serotonin uptake velocity, and the response to hypotonic stress within the separate suspensions of fresh and cryopreserved platelets. There was no indication that hypercoagulability was induced by infusion of small amounts of cryopreserved platelet

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17377196351.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17377196352.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

The plateletpheresis program of the M.D. Anderson Hospital was established in 1963. Approximately 20,000 units of platelet concentrates are produced per year. Credit for the nonreplacement fee generates the greatest number of donors. No organized recruitment campaign exists. Each donor becomes a recruiter for the program. Also involved in donor recruitment are all laboratory personnel, as well as Donor Room exployees.

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17377196353.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

Ninety Australian college students, both donors and nondonors, were asked why they felt other people did not give blood. Donors tended to stress fears, possible ill‐effects, and the inconvenience of giving blood. Nondonors tended to dismiss most reasons, although more than half of them agreed with Fear of Being Pricked with a Neddle, Never Been Asked to Give, and Inconvenience such as Loss of Tim

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17377196354.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17377196355.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

Experiments were performed to measure the stability of opsonins during storage of whole blood collected in CPD solution and stored at 4 C. Three units were collected, and aliquots removed and recalcified on days 0, 3, 7, 14, 21, and 28. Reconstituted serum was immediately frozen and stored at −70 C. The ability to opsonize Escherichia coli 075 and Pseudomonas aeruginosa immunotype 1, both of which require activation and utilization of the alternative pathway of complement for opsonization, were performed simultaneously for each organism and for each unit. Results showed that there was a slight but insignificant reduction in opsonic strength during storage for up to 28 days. The results indicate that standard collection and storage conditions using CPD as the anticoagulant retains functional activity of the alternative pathway. C3 measured by radial immunodiffusion using antisera against the B antigenic determinant showed that C3 had not been converted during storag

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17377196356.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

A technique is described which allows testing for HBsAg by hemagglutination inhibition in capillary tubes. The test is rapid, and compares favorably in sensitivity to radioimmunoassay. The equipment is simple, and its design eliminates the risk of infection from the HBsAg reagents.

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17377196357.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY

摘要:

In a pilot study the optimal concentration of inosine for 2,3‐DPG maintenance in a CPD‐adenine (0.25 mM) preservative was confirmed to be at lease 10 mM. In these experiments, 2,3‐DPG maintenance was nearly normal for six weeks of storage in CPD‐adenine‐inosine (10 mM) preservative with or without methylene blue. The control preservative lacking inosine showed a statistically significant decrease in 2,3‐DPG concentrations after the 3rd week. Finally, 2,3‐DPG levels were significantly better maintained in CPD‐adenine preservatives that contained 15 mM concentrations of inosine, whether methylene blue was present or not (10(‐6)M), compared to CPD‐adenine‐inosine preservatives that contained 5 mM inosine, with or without methylene blue. The methylene blue effect, while it can be demonstrated in most experiments to help the red blood cell maintain 2,3‐DPG during prolonged blood storage, is judges to be a slight value. However, inosine is of great value in maintaining 2,3‐DPG for prolonged (five to

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1977.17377196358.x

出版商:Blackwell Science Ltd    

年代:1977

数据来源: WILEY