摘要:

Seven serologic procedures were studied to determine their respective value in compatibility and screening tests. All seven were significantly improved by the use of 4 volumes of serum, rather than 1, with 1 volume of red cell suspension, and a low‐ionic antiglobin test (LIAGT) was distinctly superior to the other six procedures evaluated. In this test, during the incubation of serum and cells at 37 degrees for 20 minutes, ionic concentration was reduced 62 percent. However, after removal of all supernatant, the red cells were washed three times with an isotonic solution that provided 80 percent reduction in ionic concentration, and the washed cells were tested for their agglutinability with low‐ionic (80% ionic reduction) anti‐IgG antiglobin reagent. This modified LIAGT was usually more, and apparently never less, sensitive than a test described earlier and is expected to be associated with much less nonspecificity. The extreme sensitivity of LIAGT for many long‐term frozen stored alloantiserums is a retained property of the modified test and has been associated with IgG aggregation during

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27287150182.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1987.tb00917.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

A prospective double‐blind study compared a manual hexadimethrine bromide (Polybrene) antiglobulin antibody detection test (P‐AHG) and crossmatch with the albumin‐antiglobulin antibody detection test and saline‐antiglobulin crossmatch routinely used in our laboratory. A total of 10,084 pretransfusion blood samples from approximately 6000 patients were tested. The P‐AHG method detected 153 of 157 alloantibodies for which antigen‐negative, crossmatch‐compatible blood is routinely provided. All four antibodies not detected were anti‐K. The routine techniques detected 147 of the 157 alloantibodies. The P‐ AHG method detected only 36 percent of the alloantibodies for which crossmatch‐compatible blood is routinely provided without determination of the antigen status of the donor unit's red cells (e.g., anti‐Lea), whereas the routine method detected 91 percent of such antibodies. Eighty‐six percent of the 189 alloantibodies detected by the Polybrene technique were found before the addition of antiglobulin. The manual Polybrene test is a rapid and sensitive technique; it may be used without an antiglobulin phase as a routine crossmatch procedure when accompanied by a sensitive antibody detection test that includes antiglobulin and an additional test to e

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27287150183.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

The sensitivities of manual low‐ionic hexadimethrine bromide (Polybrene, LIP) and low‐ionic Polybrene indirect antiglobulin tests (LIPAT) were compared with those of a manual low‐ionic‐strength indirect antiglobulin test (LISS) by using a commercial Polybrene kit. One hundred antibodies were coded, titrated, and tested in parallel. LIP did not detect 36 antibodies: 31 anti‐K, two anti‐E, two anti‐Fya, and one anti‐Jka. LIPAT did not detect seven anti‐K, two anti‐E, and two anti‐Jka. The combination of LIP and LIPAT did not detect two anti‐ E that were reactive only in a two‐stage enzyme test and seven anti‐K that had titers of 2 or lower by LISS. LISS detected all antibodies except for the two enzyme‐reactive anti‐E. There were no significant differences in the titers of 63 percent of the antibodies studied. For 54 percent of the antibodies in the Kell system, LISS produced significantly higher titers; for 25 percent of antibodies in the Rh system, LIP did so. The poor sensitivity of the Polybrene kit for anti‐ K makes it unsuitable as a prim

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27287150184.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

Chemically modified anti‐D typing serum is considered to combine the reliability of saline IgM anti‐D with the convenience of slide and rapid tube anti‐D. This has led to its wide acceptance in modern blood bank practice. False‐positives are considered rare and are controlled by the ABO grouping. The extra step of an Rh control, which is necessary with IgG slide and rapid tube anti‐D serums, is thus eliminated. However, this report shows that adopting this practice can be misleading and dangerous. Two cases are reviewed in which the chemically modified anti‐D gave false‐positive results and the ABO typing did not act as an ade

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27287150185.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

This article describes the production of red cell alloantibodies in 13 of 150 patients after bone marrow transplantation. New alloantibodies appeared 12 days to 11 months after the transplantation. The specificities of these antibodies were anti‐N, ‐Jka, ‐E‐like, ‐Kell‐ like, ‐M, ‐Leb, ‐Hl, ‐H and ‐A1. The posttransplantation production of antibody could be due to either the transfusion of mature lymphocytes along with the marrow, the ability of the grafted immune system to produce alloantibodies, or the viable immunocompetent cells remaining despite high‐dose chem

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27287150186.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

Because graft‐versus‐host disease remains a major complication in allogeneic bone marrow transplantation, a number of techniques capable of removing mature T lymphocytes from bone marrow cells have been attempted. The authors describe a simple two‐step procedure using counterflow centrifugation elutriation (CCE) that eliminated 95 to 98 percent of the mature T lymphocytes and greater than 97 percent of the T lymphocyte colony‐forming units (CFU‐T) while concentrating the bone marrow myeloid colony‐forming cells. Viability was greater than 98 percent, and 72 to 98 percent of the total cells separated were recovered. Lymphocyte depletion was substantiated by both morphologic and phenotypic criteria using monoclonal antibodies to T lymphocytes, as well as by responsiveness in mixed‐lymphocyte cultures and to mitogens. In addition, this technique separated the lymphoid colony‐ forming cells from the larger myeloid colony‐forming cells. It was concluded that this simple two‐step CCE procedure can be used to separate T lymphocytes and CFU‐T from myeloid colony‐forming cells and offers a means of purging T lymphocytes from large numbers of marrow cells that may be required for human allogeneic bone

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27287150187.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

In order to determine whether the rise in blood circulating committed hematopoeitic progenitor cells (CFU‐GM) was sustained long enough after exercise to be a practical adjunct to increasing stem cell yields by hemapheresis, the authors evaluated two exercise protocols in 15 normal donors. Immediately after a brief period of intense exercise, there was a significant increase in the absolute number of blood CFU‐GM from 164 +/− 27 to 240 +/− 46 per ml. However, all values returned to baseline within 15 minutes. Prolonging the exercise did not enhance either the circulating CFU‐GM concentration or the duration of the rise. If blood hematopoietic stem cells respond to exercise similarly, such a transient increase would not appear to be of use in stem cell h

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27287150188.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1987.tb00924.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

It is desirable to minimize the contamination of plateletapheresis products by lymphocytes because of the role these cells may play in febrile nonhemolytic transfusion reactions, alloimmunization, platelet deterioration during storage and also to reduce donor lymphocyte loss. This study attempted to determine whether lymphocyte contamination could be reduced by rapidly depleting plasma from the separation chamber at selected intervals during plateletapheresis with a blood cell separator (CS‐3000, Fenwal). Donors who provided platelet components with more than 0.10 × 10(9) lymphocytes without rapid depletion underwent a second plateletapheresis procedure in which the rapid depletion technique was used. The plasma pump was reprogrammed to remove rapidly 12 ml of plasma from the separation chamber (pump speed 48 ml/min) immediately after the plasma pump reversal. All other collection techniques were identical for all procedures. Significantly fewer lymphocytes were collected in those procedures in which rapid depletion was used (mean, 0.187 × 10(9] than when it was not used (mean, 0.331 × 10(9](p = 0.001, n = 30). There was no effect on platelet yield, efficiency of platelet collection, processing time, or total collection time. This procedure provides a product that should be considered for routine

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27287150189.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY