摘要:

Blood was collected into Acid Citrate Dextrose solution (ACD) from a group of 20 large, adult rhesus monkeys. In ten of the experiments, the blood was labeled immediately with radioisotopes and reinfused into the donor monkeys. The other ten blood units were stored at 4 C for 21 days, then tabled and infused into the donor monkeys.In vitrodeterminations were made immediately after collection and at the end of the storage period and compared with the results obtained in stored human blood.The ten fresh blood infusions had a mean survival of 101.3 per cent with an average half time of 10.4 days. The 21 (lay‐old bloods had a mean survival of 73.7 per cent (S.D. = 5.1%) with an average half time of 11.3 days. The average red blood cell survival of 123 human blood units stored 21 days and reinfused into their donors had an average red cell survival of 81.0 per cent (S.D. = 8.6%). The determinations in rhesus blood and human blood were comparable.These data strongly suggest thatin vivostudies done in the rhesus monkey may be substituted for those performed in man to evaluate red cell preservatio

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1973.tb05456.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

A statistical study of the incidence and specificity of multiple irregular blood group antibodies shows predominance of those within the Rh‐Hr, Kell, and Duffy systems. When multiple antibodies included those in the Lewis, Lutheran, MNS, P, or Kidd systems, the other antibodies were usually in the Rh‐Hr, Kell, and Duffy systems.The following categories of donors: 1a Group O Rh‐neg. (dce/dce) K‐neg. Fya‐neg. 1b Group O Rh‐neg. (dce/dce) K‐neg. Fyb‐neg. 2a Group O Rh‐pos. (R1R1‐DCe) K‐neg. Fya‐neg. 2b Group O Rh‐pos. (R1R1‐DCe/DCe) K‐neg. Fyb‐neg. 3a Group O Rh‐pos. (R2R2‐DcE7DcE) K‐neg. Fya‐neg. 3b Group O Rh‐pos. (R2R2‐DcE/DcE) K‐neg. Fyb‐neg. are further subtyped for Lea, Leb, P, Jka, Jkb, M, N, S, s, and Lua.Erythrocytes from these donors, frozen in glycerol, with Anti‐A, and Anti‐B antibodies removed during deglycerolization simplifies the problem of finding com

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1973.tb05457.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Severalin vitroplatelet function assays were examined. Two units of platelets from each of ten volunteer donors were obtained by plasmapheresis, stored three days, and examined by various functional parameters. The second unit of platelets from an individual had generally lower functional parameters than the first, suggesting a subtle physiological change in the donors' circulation between the time of drawing the first and second units. Forin vitrocomparisons, this difference was overcome by a pooling procedure. Platelet storage was examined at room temperature and at 4 C.

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1973.tb05458.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Papainized red blood cells and red blood cells sensitized with Rh antibodies were stored at 4 C in Alsever's solution containing inosine. The papainized cells were used for detection of Rh antibodies. The cells sensitized with anti‐D were used for the positive control of the antiglobulin reaction. When stored for no more than three weeks, the preserved cells gave reactions almost identical to those obtained with freshly prepared cell

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1973.tb05459.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

A 17‐year‐old boy developed paroxysmal cold hemoglobinuria (P.C.H.) following a respiratory infection due to Mycoplasma. The Donath‐Landsteiner test was positive; the specificity of the biphasic hemolysin was anti‐I. The cold agglutinin phase of the hemolysin had specificity compatible with Marsh's concept10of anti‐IFetaIand anti‐IDevelopedas shown by reactions with group O, I+, i adult and i cord cell, and serum inhibition studies with

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1973.tb05460.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

While investigating what appeared to be an albumin‐agglutinating antibody, it was discovered that two commercial albumins were heavily contaminated with gamma globuli

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1973.tb05461.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

Examples of both anti‐M and anti‐N have been reported in patients possessing these antigens; however, in only two instances has anti‐M been reported as an autoantibody. This paper describes two additional patients with auto anti‐M. In each case the anti‐M was shown to be an autoantibody by absorption and elution studies. Optimum reactivity was obtained at 4 C. Column chromatography and 2‐mercaptoethanol reduction indicated that the antibody was an IgM globulin. There was no evidence of autoimmune hemolysis in either of the

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1973.tb05462.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

The blood of a patient with infectious mononucleosis was investigated when a discrepancy was noted between cell and serum grouping. The red blood cells of the patient were reactive with a commercial anti‐B. Her serum strongly agglutinated group A and group B cells. Anti‐B from four other commercial sources and from ten normal group A donors did not agglutinate the patient's cells.The coloring material in the commercial anti‐B with which the patient's cells reacted is yellow #5 tartrazine. The patient's serum agglutinated normal group O cells on the addition of this dye. No other example of this factor has been found in routine blood grouping or in testing the serum from 30 further patients with infectious mononucl

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1973.tb05463.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

A case of hemolytic disease of the newborn caused by Jsasensitization is reported. The antibody, the sixth example of anti‐Jsa, was detected in the serum of the mother of a nine‐day‐old anemic infant who required supportive transf

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1973.tb05464.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY

摘要:

This brief communication shows how adoption of the Radio Immune Assay (RIA) test will increase outdating of blood and also increase the average age of blood used for transfusion. The results are based on the assumption that each test will take approximately one day and that a random draw issuing policy will be applied to both free and crossmatched blood.

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1973.tb05465.x

出版商:Blackwell Publishing Ltd    

年代:1973

数据来源: WILEY