ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1983.23684074263.x

出版商:Blackwell Science Ltd    

年代:1983

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1983.23684074264.x

出版商:Blackwell Science Ltd    

年代:1983

数据来源: WILEY

摘要:

In order to relate changes in protein‐bound and complex‐bound calcium to levels of citrate and secretion of parathyroid hormone during mild hypocalcemia, total, ultrafiltrable, and ionized calcium were measured in 13 apparently healthy donors during plateletapheresis. Carboxyl‐terminal parathyroid hormone was measured in seven of the donors, and both amino‐terminal and intact parathyroid hormone assays were done on one donor. Mean concentrations of total and ionized calcium decreased during most of the procedure, as did total protein, albumin, and phosphorus. Complexed calcium increased, which was attributed to the large increases in citrate levels. Protein‐bound calcium decreased more than other forms of calcium, even allowing for the decrease expected from hemodilution. The carboxyl‐terminal parathyroid hormone response varied among different donors, but on the average, decreased slightly before gradually rising during the citrate‐induced hypocalcemia. These findings suggest that calcium citrate does not affect the secretion of parathyroid hormone, and that at least a portion of protein‐bound calcium is readily exchangeable and possibly influenced

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1983.23684074265.x

出版商:Blackwell Science Ltd    

年代:1983

数据来源: WILEY

摘要:

The effect of cardiopulmonary bypass operations on the concentration of plasma fibronectin, IgG, and C3 was studied in 28 patients. During cardiopulmonary bypass operations, plasma IgG, and C3 decreased to about 45 percent of their preoperative concentrations, probably as a result of hemodilution and blood loss. On the average, plasma fibronectin decreased significantly more (p<0.002), to 32 percent of its preoperative concentration, probably as a result of hemodilution, blood loss, and consumption. We assumed that consumption of fibronectin occurred when its concentration decreased more than the concentrations of IgG and C3. Interestingly, consumption of fibronectin during bypass operations was not demonstrated in seven of the 28 patients who did not require blood products within the 24‐hour period after operation. We suggest that consumption, rather than dilution, of plasma fibronectin might be clinically important. A significant (p<0.002) negative correlation was observed between the length of the bypass and the concentration of plasma fibronectin during this 24‐hour period.We also studied the rate of regeneration of fibronectin, IgG, and C3 in 12 of these patients. The concentrations of fibronectin and C3 were normal by the fifth postoperative day. The rate of regeneration of IgG, however, was slower than that of fibronectin and

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1983.23684074266.x

出版商:Blackwell Science Ltd    

年代:1983

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1983.tb02130.x

出版商:Blackwell Science Ltd    

年代:1983

数据来源: WILEY

摘要:

Platelet concentrates (PC) are stored for up to 5 days at 22° C prior to infusion. Since considerable suspending plasma is infused with the platelets, we examined the integrity of plasma fibrinogen from stored PC. The concentration of fibrinogen after storage was normal. After purification, fibrinogen from stored PC had normal thrombin time and rate of polymerization of fibrin monomer, and after reduction, its Aα, Bβ, and y chains had normal mobility on sodium dodecyl sulfate polyacrylamide gel electrophoresis. Assays of plasma from stored PC for fibrinogen‐fibrin degradation products were negative. When125l‐fibrinogen was added to PC prior to storage and supernatant plasma was filtered in a sepharose 4B column after storage, radioactivity eluted in a single, symmetrical peak with no evidence for formation of low or high molecular weight material. These results make it unlikely that thrombin, plasmin, or other proteolytic activity is generated during storage. The levels of factors V and VIII fell to 40 to 65 percent of control values while the activities of factors IX, X, and XI did not change significantly during storage. We conclude that suspending plasma fibrinogen and other coagulation factors are remarkably stable during PC storage. They should be of value during the support of patients with massive hemo

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1983.23684074267.x

出版商:Blackwell Science Ltd    

年代:1983

数据来源: WILEY

摘要:

Dr. Croone told me, that at the meeting of Gresham College tonight, which it seems they now have every Wednesday, there was a pretty experiment of the blood of one dog let out till he died, into the body of another on one side, while all his own run out on the other side. The first died upon the place, and the other very well and likely to do well. This did give occasion to many pretty wishes, as of the blood of a Quaker to be let into an Archbishop, and such like; but, as Dr. Croone says, may, if it takes, be of mighty use to man's health, for the amending of bad blood by borrowing from a better body.

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1983.tb02132.x

出版商:Blackwell Science Ltd    

年代:1983

数据来源: WILEY

摘要:

Citrate‐phosphate‐dextrose‐adenine (CPDA‐1), containing 0.25 mMadenine (final concentration) and 25 percent more glucose than citrate‐phosphate‐dextrose (CPD), has extended the allowable storage time for red cells to 35 days. Studies were conducted to understand better the characteristics of stored CPDA‐1 red cells in relation to the properties of stored CPD red cells. Units with hematocrits near 80 percent showed the following: First, adenosine triphosphate (ATP) and total adenine nucleotide levels of red cells stored with CPDA‐1 remained essentially constant during the first 3 weeks of storage after which the levels decreased; with red cells stored with CPD, ATP, and adenine nucleotide, levels were decreased even after 1 week of storage. Second, the pattern of the fall in 2,3‐diphosphoglycerate was similar in red cells stored with CPD and CPDA‐1. Third, changes in plasma and red cell levels of sodium and potassium, and in plasma ammonia levels, were comparable in CPD and CPDA‐1 units; changes in cation levels were most pronounced during the initial 2 weeks of storage. Fourth, hemolysis was much greater in units stored in CPDA‐1 for 35 days than in units stored in CPD for 21 days. Fifth, residual glucose concentrations were adequate in units drawn in CPDA‐1 and stored for 35 days. We conclude that the changes in the biochemical characteristics of units of red cells stored with CPD and CPDA‐1 are similar in most instances with the notable exception of the better maintenance of adenosine triphosphate levels in r

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1983.23684074268.x

出版商:Blackwell Science Ltd    

年代:1983

数据来源: WILEY

摘要:

We performed a retrospective study of 167 women undergoing hysterectomy in a large tertiary‐care teaching hospital in order to assess intraoperative blood requirements and to study the decision‐making process that occurs when blood is transfused intraoperatively. Recommended preoperative blood orders for hysterectomy patients based on data from the study are provided. Although “type and screen” is commonly considered to be an adequate preoperative order for patients undergoing vaginal hysterectomy, our data indicate that 2 units may be a more appropriate order when the procedure is combined with pelvic floor repair. The preoperative hematocrit, the estimated intraoperative blood loss, and the duration of the hysterectomy were integral to the decision‐making process. If women undergoing hysterectomy were transfused intraoperatively only when their hematocrit actually fell to 30 percent, and not in anticipation of such an event, unnecessary transfusions would b

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1983.23684074269.x

出版商:Blackwell Science Ltd    

年代:1983

数据来源: WILEY

摘要:

A method for obtaining granulocytes for transfusion by direct aspiration of bone marrow from normal donors is described. The bone marrow is the major storage reserve for granulocytes with as many as 60 times more granulocytes than in the peripheral blood. With current methods of leukapheresis from peripheral blood, steroids, hydroxyethyl starch, and anticoagulants are given to the donor and his processed blood is returned. Bone marrow aspiration spares the donor such treatment. In addition, no costly machine is necessary and the whole procedure takes less than 2 hours. Due to minimum manipulation, cell viability is not impaired. Furthermore, some committed granulocyte stem cells, as well as nonmature granulocytes, are transfused. These cells have the potential to mature to efficient phagocytes in the patient's circulation. TRANSFUSION 1983;23:496–49

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1983.23684074270.x

出版商:Blackwell Science Ltd    

年代:1983

数据来源: WILEY