ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1982.tb02000.x

出版商:Blackwell Science Ltd    

年代:1982

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1982.tb02001.x

出版商:Blackwell Science Ltd    

年代:1982

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1982.22182154214.x

出版商:Blackwell Science Ltd    

年代:1982

数据来源: WILEY

摘要:

The study was undertaken to assess the potential of a labeled C3d radioimmunoassay method as a standardization criterion for antiglobulin reagents containing anti‐C3d reactivity. Over an 18‐month interval, four different lots of broad‐spectrum antiglobulin reagents were purchased from each of seven United States manufacturers. Anti‐C3d antibody concentration and Ko in each lot were assayed initially and at four‐month intervals over 16 to 21 months storage at 4 degrees C. The results permitted 1) comparison of anti‐C3d antibody concentrations among different manufacturers, 2) comparison among different lots from the same manufacturer, and 3) assessment of stability of anti‐C3d during storage. In addition, serial dilutions of reagents in each manufacturer's diluent were compared for their anti‐C3d agglutinating properties by the spin‐antiglobulin method against red blood cells (RBC) coated by C3d in vitro and against in vivo C3d‐coated RBC from 16 patients. Most reagents were shown also to contain anti‐C3c, anti‐C4c and anti‐C4d reactivities when tested against suitably coated RBC. Anti‐ C3d antibody concentrations ranged from approximately 1 to 3.5 microgram/ml for 27 of the 28 reagents and were stable over 16 to 21 months of storage. Anti‐C3d agglutination titer scores showed a general correlation with anti‐C3d antibody concentrations when tested against in vitro C3d‐coated RBC; correlations were less good against C3d‐coated cells from patients. The radioimmunoassay provided reproducible objective measurements and appeared to have m

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1982.22182154220.x

出版商:Blackwell Science Ltd    

年代:1982

数据来源: WILEY

摘要:

Nine unexpected antibodies of unquestioned clinical significance were detected when the major crossmatch was performed on 31,320 pretransfusion blood samples from 8969 patients whose screening test for unexpected antibodies was nonreactive. Three of the antibodies retrospectively were found to manifest a positive screening test. Another antibody was not detected by the antibody screening test due to an error in preparation of the screening red blood cells. The overriding importance of the major crossmatch is the assurance of ABO compatibility between donor blood and recipient. Therefore, while this study does not resolve whether the antiglobulin phase of the procedure might be considered optional, the major crossmatch should not be eliminated.

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1982.22182154207.x

出版商:Blackwell Science Ltd    

年代:1982

数据来源: WILEY

摘要:

The effects of transfusion on the rheological properties of blood in sickle cell anemia were studied in 15 patients. Blood samples were obtained before and after transfusion of normal (hemoglobin AA) packed cells. Blood viscosity was determined with a coaxial cylinder viscometer over a wide range of shear rates. The index of oxygen dependence of blood viscosity (eta O2) was calculated as the ratio of viscosity values at low PO2 (20 mm Hg) and at high PO2 (100 mm Hg) levels. After transfusion, blood viscosity significantly increased as a result of an evaluation of hematocrit. Although transfusion of normal cells into sickle cell anemia patients results in an increased oxygen content of blood and a decreased oxygen dependence of blood viscosity, an elevation of hematocrit value beyond 35 per cent may cause a high viscosity state and outweigh the benefits of transfusion. Judicious monitoring of blood viscosity should serve as a guide for blood transfusion in these patients.

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1982.22182154208.x

出版商:Blackwell Science Ltd    

年代:1982

数据来源: WILEY

摘要:

To determine the effect of microaggregate blood filtration on patients with compromised pulmonary function, we divided 50 patients having elective coronary bypass surgery into two groups. One group received all blood transfusions via a 170‐micron standard filter (SF). The other group received blood through a 20‐micron microaggregate blood filter (MF). Patients were monitored pre‐ and postoperatively for changes in arterial blood gases and cardiopulmonary function. Mean transfusion volume was seven units (SF) and six units (MF). Preoperatively, patients in both groups showed abnormal lung function with elevated intrapulmonary shunting (Qs/Qt) and alveolar‐arterial gradients (A‐ aO2). Postoperatively, no significant differences (p greater than 0.05) were found between the groups for any of the tests of cardiopulmonary function evaluated. Posttransfusion, none of the patients in either group showed clinical signs of respiratory distress. We conclude that even for patients with some degree of pretransfusion pulmonary dysfunction, use of a microaggregate blood filter for six‐ to seven‐ unit transfusions does not provide significant cli

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1982.22182154209.x

出版商:Blackwell Science Ltd    

年代:1982

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1982.tb02007.x

出版商:Blackwell Science Ltd    

年代:1982

数据来源: WILEY

摘要:

Density distribution curves of red blood cells (RBC) from patients with a positive direct antiglobulin test (DAT) were compared to a standardized curve constructed from cell column measurements of centrifuged microcapillary tubes filled with RBC and phthalate ester mixtures encompassing a specific gravity range of 1.078 to 1.114. Shortened survival resulted in a loss of older RBC and a shift of the curve to the right over the high specific gravity ester range. Reticulocytosis resulted in a downward shift of the curve over the low specific gravity range. In patients with a positive DAT due to an autoantibody or drug, the density distribution curve was either normal or demonstrated evidence of shortened RBC survival. In patients with a positive DAT due to an alloantibody, however, evidence of shortened survival was not seen. The distribution of antibody on young and old RBC harvested from the appropriate microcapillary tubes depended upon the etiology of that antibody. In patients with a positive DAT due to to an alloantibody or drug, both the young and old RBC gave an equally reactive DAT, while in patients with a positive DAT due to an alloantibody the young cells were weakly reactive or nonreactive and the older cells were more strongly reactive. When used together, the position of the density distribution curve and the pattern of distribution of antibody coating on young and old RBC provide important diagnostic information about the etiology and clinical status in a patient with a positive DAT and allow for the recognition of an alloantibody and autoantibody when both are present in the same patient.

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1982.22182154210.x

出版商:Blackwell Science Ltd    

年代:1982

数据来源: WILEY

摘要:

Three examples of autoantibodies with Jka specificity are reported. They all react preferentially by low‐ionic strength salt (LISS) techniques, but only in the presence of parabens (butyl, ethyl, methyl, and propyl esters of p‐hydroxybenzoate) or certain other neutral aromatic compounds. They were detected through the use of commercially prepared LISS solution containing parabens as preservatives. While it is unlikely that these autoantibodies are of clinical significance, the parabens can now be added to the list of reagent preservatives that may give rise to anomalous serologic reacti

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1982.22182154211.x

出版商:Blackwell Science Ltd    

年代:1982

数据来源: WILEY