ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00030.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

A few of the factors responsible for the preservation of the characteristics of fresh blood during storage have been studied, with particular reference to the provision of donor blood for open heart surgery. It has been indicated that present data leave us no reason to think that platelet viability and the level of labile clotting factors in stored blood will be any different whether the blood is collected by gravity or with the aid of vacuum. Changes in bloods stored at 4C. for a 21 day period were investigated with ACD, heparin and Edglugate‐Mg as the anticoagulants. The studies on heparinized blood suggest that after two or three days, the fall in platelets and the rise in plasma potassium and hemoglobin are so much greater than occur in ACD that storage for longer periods than that will probably never be safe. On the other hand,in vivostudies indicate that 24‐hour‐old heparinized blood is just as safe to use as four‐hour‐old blood. Edglugate‐Mg was developed in an effort to get around the limited storage period of heparinized blood. Results reported here show that plasma potassium levels rise very rapidly in this type of blood. Platelets are not well‐preservedin vitro, and Factor V levels are apparently low soon aft

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00031.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

The bloods of six healthy young male students were stored anaerobically in the cold and at intervals the stroma‐free red cell hemolysates were analyzed in a cacodylate buffer atpH 6.5 and .05M concentration. From these data, a control range was established.Results are presented which show the effect of anaerobic storage on the B/2 values of red cell hemolysates of the bloods of 23 patients with a variety of diseases. In 21 cases, the B/2 values decreased rapidly or showed an initial increase followed by a decrease. Attempts to relate the type of storage curve to specific diseases were unsuccessful. It is concluded that water‐soluble protein components of the red cells may be altered by dise

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00032.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

When mixtures of stained and unstained human red cells of different antigenic composition are mixed with homologous non‐saline agglutinating antisera in a small Petri dish, two distinctly different clumps of cells are formed. This observation suggests that the agglutinating phase of the reaction is specific in accord with the Marrack hypothesis and further implies that these antibodies are not univalent but are bi‐ or polyvalent. As a result of formation of a single cell clump, application of this technic has led to the discovery of a serum containing a heretofore unrecognized anti‐CD ant

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00033.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

The plasmacrit (PCT) is a modification of the VDRL slide test for syphilis in which plasma instead of serum is used as the source of reagin. The plasma inhibitory component is neutralized by using 10 per cent choline chloride as the diluent in the preparation of the VDRL antigen suspension. We performed the PCT and VDRL simultaneously on the plasma and serum of 2,458 individuals. There was complete agreement between the two tests in 99.4 per cent of the total number tested. Different results were obtained with 15 specimens and with only one exception, these results indicated a greater sensitivity for the PCT. We believe that the PCT may be satisfactorily employed as a screening test in a clinical laboratory or blood bank.—.A. Balow

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00034.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

The effect of molarity of NaCl, KC1, LiCl, tris(hydroxymethyl)aminomethane acetate, CaCl2, MgCl2, Na2SO4, K2SO4) and MgSO4solutions on the agglutination of human erythrocytes by isohemagglutinins of the ABO series has been studied, as has that ofpH in “tris acetate” buffer. In all cases the maximum agglutination titer occurred at the isotonic or a slightly hypertonic concentration and was generally the same as that determined by diluting the given cells and serum with isotonic saline. Moreover, all salts exerted quantitatively similar effects on agglutination, the titer decreasing regularly as the molarity increased from 0.1544‐308 to 1.0. Apparent variations in the effects of different classes of salts were induced by expressing salt concentration as ionic strength or normality rather than molarity.In tris acetate buffers of ionic strengths 0.154 (isotonic) and 0.308, the titer was constant in thepH range 5.5 to 7.5–8.0 and decreased at either

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00035.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

Analysis was made of isoantibody in the serum of Mrs. Peltz, who is of the very rare Kell type K—,k—,Kp(a—b—). Successive adsorptions and elutions were done using three bloods selected in such a way that the eluate finally obtained could contain no previously known blood‐group antibody. It agglutinated all of 525 bloods chosen at random, and failed to agglutinate only very rare bloods that were of highly unusual K

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00036.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00037.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

In control experiments small volumes of incompatible, chromium51tagged red cells were given to recipients. Several days later the studies were repeated 30 minutes after the intravenous administration of prednisolone. Prednisolone was found completely ineffective in prolonging the survival of small amounts of red cells in the presence of incompatible antibodies of the ABO and Rh systems.

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00038.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

For the past four years, the method presented here for the detection of leukoagglutinins has been successfully integrated into the routine procedures of a community blood bank. Patients' sera are tested against leukocytes from 12 Group O donors. White cell suspensions are prepared from defibrinated blood using 4 per cent polyvinylpyrrolidone (PVP) as a sedimenting agent. Tests have been performed on 276 patients, among whom 89 (32 per cent of the total) were found to possess leukoagglutinins. Of the 89 individuals in whose sera leukoagglutinins were demonstrated, 85 had a reported history of febrile transfusion reactions, frequently accompanied by chills, occasionally with urticaria. These 85 patients made up 57 per cent of 150 cases who had been reported as experiencing nonhemolytic febrile reactions shown by several workers to occur in patients with leukoagglutinins.Also described are methods used for the preparation of leukocyte‐poor units requested for patients whose sera have been shown to contain leukoagglutinin

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00039.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY