ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27187121448.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

A thrombocytopenic,leukopenic patient with multiple myeloma who was given 7 units of platelets died 6 days later from complications ofSalmonellaheidelberg septicemia. A platelet donor who was asymptomatic at the time of donation had group BSalmonellaon stool culture. His clinical history and the results of serologic studies and stool culture were consistent with a mildSalmonellagastroenteritis 5 days before donation. Antibiotic sensitivity patterns and plasmid profiles indicated that the organism (S.heidelberg) isolated from the donor's stool was identical to that isolated from the patient's blood and from the platelet bags. It is believed that low‐grade, asymptomatic bacteremia in the donor was the source of infection in the recipient. Food and Drug Administration records contain reports of six septic deaths due to platelet transfusions since 1979, compared with none in the preceding 4 years. Increased use of platelet products and the standard practice of storage at room temperature may contribute to the risk of sepsis after platelet transfusion, particularly in immunocompromised patient

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27187121466.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1987.tb04013.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

Platelet concentrates stored for 7 days in 50 ml of plasma in both thin film and enlarged variations of the standard 5‐day CLX plastic bags were evaluated for pH maintenance and in vivo viability by two laboratories working independently.51Cr‐labeled platelets were reinfused into normal volunteers at the end of storage and recovery and half‐life calculated. The pH was maintained well;<10 percent of units fell below 6.0 at 7 days. Mean 7‐day recovery for both laboratories was 43.6 ± 11.6 percent in the thin‐film bag and 45.4 ± 8.52 percent in the enlarged bag, compared with 43.6 ± 8.8 percent at 5 days in the 5‐day plastic licensed bag. After 7 days storage the half‐ life was 3.6 ± 0.9 days in the thin‐film bag and 3.7 ± 0.6 days in the enlarged bag, compared with 3.6 ± 0.5 days in the previously licensed CLX plastic bag after 5 days. Thus, platelet viability was maintained well at 7 days of storage in both of the container variations that allowed i

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27187121476.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1987.tb04015.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

A paired prospective study was performed to compare the in vitro storage characteristics and in vivo kinetics of platelets stored in granulocyte‐platelet concentrates prepared by apheresis with platelets prepared from whole blood. Platelet and granulocyte‐platelet concentrates were collected from five healthy volunteer autologous donors and stored for 16 to 18 hours at 20 to 24°C with and without agitation, respectively. After storage, pH, platelet count, percent release of β‐thromboglobulin, morphologic score, and percent osmotic recovery were measured. In addition, the granulocyte‐platelet concentrates were assayed for total leukocyte count, release of lysozyme, and by several in vitro tests of granulocyte function. The platelets in both products were labeled with 111In oxine and infused into the donors. The pH of both products was above 6.0 at the end of storage. The units stored as platelet concentrates compared with those stored as granulocyte‐platelet concentrates showed a higher percent release of β‐thromboglobulin, 18.4 ± 4.0 percent versus 5.9 ± 3.2 percent (mean ± SD), but significantly better morphologic scores, 676 ± 21 versus 525 ± 56, and better osmotic recovery scores, 72 ± 10 percent versus 40 ± 7 percent, respectively (all p<0.05). The platelet concentrates (compared with the granulocyte‐ platelet product) had significantly better in vivo recovery, 49.5 ± 15.8 percent versus 38.9 ± 11.5 percent, and survival, 6.1 ± 1.3 days versus 2.4 ± 0.4 days, respectively (p<0.05). After 16 to 18 hours of storage at 20 to 24°C the platelets in granulocyte‐platelet concentrate show impaired in vitro storage characteristics as well as significantly decreased in vivo recovery and survival especially if agitated during storage, compared with age‐matched ra

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27187121449.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

A sensitive and specific radioimmunoassay was used to determine whether human platelets possess antigens of the MNSs blood group. Mouse monoclonal IgG anti‐M and anti‐N were purified by Staphylococcus protein A chromatography, labeled with125I, and incubated with platelet pellets from donors of various MN phenotypes. Human IgG anti‐ M, ‐S, and ‐s were purified by absorption‐elution, incubated with platelet pellets from donors of different MNSs phenotypes, washed, and incubated with125I‐labeled mouse monoclonal anti‐human IgG. In both assays, the platelet pellets were centrifuged through phthalate ester oils and the radioactivity in the pellets was counted. Dose‐response curves and ligand bound per cell indicated no significant difference in the binding of mouse or human anti‐M and anti‐N to platelets from donors of the MM, MN, or NN phenotype or of human anti‐S and anti‐s to platelets from donors of the Ss or ss phenotype. Contrary to many previous studies, our data indicate that the MNSs antigens are not expressed on the circulating human platelet. Therefore, antibodies to these antigens probably do not play a role in refractorines

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27187121464.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1987.tb04018.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

Colony‐forming units‐granulocyte/monocyte (CFU‐GM) harvested from normal donors as a byproduct of plateletapheresis can be cryopreserved successfully with 10 percent dimethyl sulfoxide with the use of standard protocols that have been developed for freezing bone marrow. Short‐term storage of CFU‐GM in polypropylene vials in the liquid phase of liquid nitrogen yielded a recovery rate of 88 ± 5 percent (mean ± SEM). Significant loss of committed progenitor cells was not detected until 1 year after freezing (65 ± 5%). A comparison of CFU‐GM recovery with the polyolefin bag technique (107 ± 13%) was not statistically different from that obtained with polypropylene vials (78 ± 7%). Although the freezing bags are more expensive and prone to fracture than the vials, they are easier to handle, store, and thaw in the laboratory, and reinfusion to the patient is more convenient. Cryopreservation of CFU‐GM harvested from peripheral blood appears feasible in either of tw

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27187121465.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY

摘要:

A 20‐year‐old man with chronic granulomatous disease (CGD) and who was receiving granulocyte transfusions for a refractory liver abscess was studied to compare the kinetics of111In‐labeled granulocytes with those of two functional granulocyte assays, nitroblue tetrazolium reduction and chemiluminescence. Transfused granulocytes were eliminated in both rapid and slow phases. Peak recovery was noted in the first sample, which was obtained 10 minutes after transfusion for each assay. The elimination kinetics were similar over 24 hours. These results confirm the value of using111In‐labeled granulocytes as a marker of transfused granulocytes. These data also confirm that the oxidative metabolic function of granulocytes prepared by continuous‐ flow leukapheresis remains intact while in the recipient's circulation. The response of the patient adds support for the use of granulocyte transfusions in certain patients

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1987.27187121467.x

出版商:Blackwell Science Ltd    

年代:1987

数据来源: WILEY