摘要:

The theme of this issue is the preservation of plasma clotting factors. Dr. Aggeler was invited to prepare an editorial on this subject. He became so engrossed that before he knew it his editorial had grown to the proportions of this review which is presented as an “editorial review.” (Edi

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00017.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

Small aliquots of plasma in 3.8 per cent trisodium citrate were stored at −15, −30 and −50 C. Lengthening of the recalcification time after 24 hours followed by progressive shortening for 10–14 days was observed at −15 and −50 C. At −30 C. no significant changes occurred until two months had elapsed. Assay of clotting factors VII and X, VIII and IX revealed essentially that the activity of each rose gradually over a span of 12–14 days after an initial decline at −15 C. and −50 C. At −30 C. factors VIII and IX were stable for at least two months. Factor V level declined gradually at −15 C. and −50 C. but no significant fall was observed at −30 C. The increase in the activity of factor VIII observed during storage at −15 and −50 C. was eliminated by prior adsorption with Celite which is believed to remove an activation product of Hageman factor and Plasma Thromboplastin Antecedent. There seemed to be somewhat greater instability of plasma stored in untreated glass containers than in cellulose nitrate or in siliconized glass. Storage temperatures below −40 C. and above −25 C. appear to be unfavor

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00018.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

Plasma stored at −50 C. or lower develops a resistance to heparin as demonstrated by progressive shortening of the thrombin time. Adsorption with Celite powder or barium sulfate before or after freezing eliminates this effect. Plasma kept at −30 C. did not develop resistance to heparin after seven d

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00019.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

The transfusion of fresh or fresh frozen plasma serves as a specific means to correct in part and temporarily the coagulation defect in hemophilia A, hemophilia B, hereditary hypoprothrombinemia vera, and congenital deficiencies of labile, stable and Stuart‐Prower factors (factors V, VII and X). The effect of a transfusion can usually be measured by simple tests such as the prothrombin time and the prothrombin consumption time. To correct thrombocytopenia by transfusion, platelet‐rich plasma should be given. Whole blood should only be used when the bleeding is massive and continuous or if the hemoglobin is very low. Transfusion of either whole blood or plasma into patients who have congenital or acquired anticoagulants in the blood, such as heparin or antithromboplastinogen (AHG or factor VIII), is ineffective and is likely to accentuate particularly the latter condit

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00020.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

A family with seven Vel negative (VeVe) members in three generations was found because the propositus had a hemolytic transfusion reaction. She had had four full‐term pregnancies and two miscarriages and one previously uneventful transfusion. The anti‐Vel antibody in her serum gave both hemolysis and agglutination. Study of the blood groups of the family shows that Veais not in the MNS system. Six of theVeVeindividuals were tested with anti‐Fyaand all were found to be Fy(a+). Some Ve(a+) bloods were noted to react more strongly with anti‐Veathan others. The explanation of van Loghem and van der Hart that the differences in the intensity of Ve(a+) reactions is due to subgroups, the stronger termed Ve(a1) and the weaker Ve(a2), is accepted by the

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00021.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

Examples of blood group antibodies were subjected to density gradient ultracentrifugation and anion‐cation chromatography on modified cellulose adsorbents and three fractions were collected. The first fraction owed its serologic activity to the presence ofγ2globulins with a 7S sedimentation constant; the principal serologically‐active component in fraction two wasγ1globulin of the 7S class; in most cases the serologically‐active component of the third fraction wasγ1globulin of the 19S class. Two anti‐A donors were found to have most of the serologic activity in fraction three before injection of blood group substance and a marked rise in fractions two and three after stimulation. In anti‐M, anti‐P and saline anti‐D sera the serologic activity associated with S19 globulins equalled or exceeded the activity with the S7 globulins. Group O serums were studied before injection of A and B substances and S7 activity was preponderant in 48 per cent whereas post‐immunization it was preponderant in 88 per cent. Fraction one activity was also preponderant in examples of anti‐K, anti‐Fya, anti‐s, anti‐Jkaand anti‐Good. Those antibodies which are most likely to cause erythroblastosis fetalis are those which are characterized by a predom

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00022.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00023.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

摘要:

Mild hemolytic disease developed in the second child of a woman who gave no history of transfusions. The antibody was identified as anti‐Jkb. The antiglobulin method gave the strongest reactions but the antibody also agglutinated test cells in saline at room temperature and at 37 C. No difficulty was encountered in determining the presence of the Jkbantigen in cord blood erythrocyte

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00024.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00025.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1961.tb00026.x

出版商:Blackwell Publishing Ltd    

年代:1961

数据来源: WILEY