ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1962.tb00226.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

Modified equipment is described for use in preparation of buffy coat‐poor red cell suspensions by the dextran sedimentation procedure. The chief advantages over previously available equipment are: (a) Minimized contamination hazard, (b) Administration of the final red cell suspension from the original blood container, thereby assuring maintenance of correct donor identification. Experience with the modified equipment in preparation of 70 units of buffy coat‐poor red cells for transfusion is described. Experience with transfusion of 297 units of buffy coat‐poor red cell suspensions at Barnes Hospital during the past five years is briefly rev

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1962.tb00227.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

Patients who have been sensitized to leukocytes by transfusions and pregnancy are subject to “pyrogenic” reactions of varying severity following the administration of whole blood. Present methods for preparing buffy coat‐poor blood utilize differential centrifugation or sedimentation in the presence of dextran. They are time consuming and not uniformly dependable.The procedure described removes the granulocytes from heparinized whole blood by passing it over a column of Nylon fibers in ten to 15 minutes. Only 5 per cent of the donation is retained in the column and the white blood counts of the final product are 500‐3,100/cu. mm. with 95 to 100 per cent lymphocytes. Cr51survival studies suggest that these preparations withstand storage satisfactorily for 14 to 21 days.Our experience with the transfusion of 82 units of this product and the relative importance of lymphocytes and granulocytes in leukoagglutination are di

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1962.tb00228.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1962.tb00229.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

The findings in 18 cases of individuals with Rho‐positive blood who had antibodies in their serum resembling anti‐Rhoin specificity are briefly summarized. One interesting finding is the relatively high frequency of such individuals among Negroes, especially with the blood type Rhdoand Rhabdo. The significance of these findings is discus

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1962.tb00230.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

In a survey of 18,365 bloods for the incidence of the Dufactor, in a population comprised approximately of 72 per cent Caucasoids, 15 per cent Negroids, 10 per cent Mexicans and 3 per cent Orientals, 78 Dubloods were found of which seven were type ccDuee. Of the latter, five came from Negroids and two from Caucasoids; the frequency among the two populations approximates respectively one in 6,000 and one in 500. In the general population of this region it is one in 2,500.The degree of reactivity of the type ccDuee found in this study was that of the “low grade” variety. However, from the remaining 71 “low grade” Dubloods, substantially weaker examples were found among type CcDuee and ccDuEe. The indirect antiglobulin and ficinized cell method readily detected type ccDuee; however, among the weaker variants, the antiglobulin method gave the more consistent results.It is concluded, therefore, that type ccDuee does occur in this area with a frequency that is considered significant and that its detection rests on the antiglobulin or other equally sensitive

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1962.tb00231.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

A series of experiments to assess the influence of several variables in a direct tube test for the Dufactor and a set of conditions that give optimal results are reported. The most important of the latter is the maintenance of a high protein content. The reagent should have a 25 per cent albumin concentration; its titer should approximate 1/128 and it should be specific, particularly for the various types of weak “low grade” Du. The applicator stick method for transferring the unknown cells from clotted or whole blood reduces the dilution of reagent that occurs when one drop of a light cell suspension in serum is used. Centrifugation at the relatively high R.C.F. of 1,200 is the other salient point of the test; following this, the test is completed by the addition of one drop of saline and a direct reading.A scheme is proposed for the application of the direct Dutube test, in conjunction with an anti‐CDE reagent, to typing donor bloods. It permits, in one direct test, the division of D negative bloods into a group consisting exclusively of type ccddee and a small group which includes all combinations of D negative bloods having factors C, Duand E. The phenotypes of the latter bloods can then be readily established in conjunction with routine methods and the direct Dutube test.Using the direct Dutube and the indirect antiglobulin methods in parallel tests of 28 Dupositive bloods, the former was the equal in accuracy and sensitivity of the antiglobulin test. The conditions which lead to false positive reaction with the tube test also gave false positive reactions with the antiglobulin test. With cells of very “low grade” type CcDuee and/or ccDuEe, false negative reactions were encountered with both methods. However, 13 examples of low grade type ccDuee bloods were studied; all gave relatively strong positive reactions with both methods.These studies indicate that the direct Dutube method is deserving of trial under the conditions of a routine blood typing program, particularly as experienced in the collection of large numbers of donor bloods. They also suggest that the test will be found the equal of the indirect antiglobulin test both in sensitivity and accuracy at a significant reduction in technician time and

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1962.tb00232.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

Whole human blood collected in heparin and glucose was incubated for up to 48 hours. Glycolysis proceeded, giving rise to lactic acid formation, and in consequence thepH decreased continuously. Blood ATP levels also decreased as glycolysis slowed since glycolysis is the only energy source in the red cells of whole human blood. The decrease in glycolytic rate was shown to be entirely dependent upon the increasing acidity and not on the lactateper se, since added neutral lactate had no effect on the rate of glycolyis or the ATP level whereas added lactic acid had a profound effect on both glycolysis and ATP level.

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1962.tb00233.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

摘要:

A white individual of European origin has been found whose erythrocytes, though they are diagnosed as M by a variety of absorbed rabbit sera, give a strong reaction with apparently N‐specificBauhinialectin. For reasons given in the text, the authors feel that this is probably not due to the presence in the erythrocytes of the individual in question of a variant of the N antigen, but of another antigen not closely related to N. The anti‐N ofVicia gramineadoes not agglutinate the cells of this individ

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1962.tb00234.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY

ISSN:0041-1132    

DOI:10.1111/j.1537-2995.1962.tb00235.x

出版商:Blackwell Publishing Ltd    

年代:1962

数据来源: WILEY