ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1979.19279160277.x

出版商:Blackwell Science Ltd    

年代:1979

数据来源: WILEY

摘要:

Two automatic apparatuses utilizing a dual wavelength spectrophotometry method were developed to perform oxygen dissociation curves on microsamples of blood. This method provides a complete printout of an oxygen dissociation curve in 15 to 20 minutes and the P50values obtained agree closely with those obtained by classical methods. This technique was used as part of a routine clinical evaluation in assessing oxygen delivery in anemic patients. These apparatuses allowed identification of patients with well‐compensated anemia, patients with no compensatory decrease in oxygen affinity, and several patients with a paradoxical increase in oxygen affinity. Knowledge of the oxygen‐releasing capacity of the blood permits a more thorough evaluation of oxygen transport in anemic patients than the hemoglobin level and a more precise assessment of the need for transfusion therapy. The ready availability of an oxygen dissociation curve by this method is feasible for routine clinical evaluation and permits more judicious use of blood produ

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1979.19279160278.x

出版商:Blackwell Science Ltd    

年代:1979

数据来源: WILEY

摘要:

Platelets eluted from nylon fiber filters after filtration leukapheresis have been studied. The platelet yield from 61 routine donations was 1.25 ± 0.18 × 10,11(mean ± SEM) corresponding to 1.78 × 1010per 500 ml blood processed. Filtered platelets labeled with radiochromate demonstrated reduced recoveryin vivo15 minutes after infusion (38.5 ± 1.7%) when compared to the control value (68.5 ± 6.8%, p<0.001). The survival of those platelets remaining in the circulation after 15 minutes did not however differ from the control value. ADP (10 μM, 100 μM, 1 mM), adrenaline (100 μM) and collagen (7.25 mg/ml) addedin vitroinduced less aggregation of filtered platelets than normal control platelets and electron microscopy revealed structural abnormalities. It is concluded that recipients of granulocyte transfusions obtained by filtration leukapheresis are unlikely to be benefited by the platelets contained in these tran

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1979.19279160279.x

出版商:Blackwell Science Ltd    

年代:1979

数据来源: WILEY

摘要:

Human granulocytes were isolated from 120 ml of whole blood by a modified counterflow centrifugation‐elutriation (CCE) technique. Overall recovery of isolated granulocytes averaged 2.82 ± 0.25 × 108cells or 77 per cent yield from whole blood with 96 per cent granulocyte purity and 4 per cent mononuclear leukocytes. The granulocyte fraction was assayedin vitroto determine chemotactic response, stimulated oxygen consumption in the presence of latex beads, bactericidal capacity, and enzyme activities. Cellular integrity was determined by scanning and transmission electron microscopy as well as by cell volume analysis. The data suggest that granulocytes isolated by CCE suffered no discernible loss of function or morphologic damage. The granulocytes are free of platelets and most mononuclear leukocytes and erythrocytes, and have not been exposed to sedimenting agents or surface adhesive agents such as dextran, nylon or glass w

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1979.19279160280.x

出版商:Blackwell Science Ltd    

年代:1979

数据来源: WILEY

摘要:

A retrospective study of the serum of 104 patients treated at Chelsea Naval Hospital between 1969 and 1972 was done. The donor blood products were not tested for the hepatitis B antigen before transfusion. The incidence of hepatitis B antigen following transfusion was about 2.8 per cent. The incidence of antibody to HBsAg prior to transfusion was 16 per cent, and about 27 per cent of the patients developed antibody to HBsAg following transfusion. The incidence of antibody to cytomegalovirus was about 22 per cent before transfusion, and 22 per cent of the patients developed complement fixing antibody against cytomegalovirus after transfusion. Since the patients received a variety of blood products it was not possible to determine retrospectively which product, if any, produced the lowest incidence of hepatitis B antigen and transmission of cytomegalovirus.

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1979.19279160281.x

出版商:Blackwell Science Ltd    

年代:1979

数据来源: WILEY

摘要:

Plasma DEHP concentrations were measured weekly in whole blood and red cell concentrates (RCC) during 21 days of storage in standard CPD within PL‐130 blood bags. In addition, DEHP and MEHP accumulation patterns were investigated in blood stored for 42 days in modified CPD with adenine within PL‐146 and BB‐69 storage containers. Total per‐unit plasma DEHP of RCC units was 49 to 71 per cent of the total in plasma of whole blood units (PL‐130). From 28 to 42 days, mean DEHP levels were 12 to 19 per cent higher in whole blood stored in PL‐146 than in BB‐69. Although MEHP was not found in any blood bag plastic, MEHP accumulated in plasma during whole blood storage. MEHP concentrations were 2.8 to 3.8 times higher in plasma stored in BB‐69 than in PL‐146. It is postulated that MEHP arises from hydrolysis of DEHP by plasma lipase, even in frozen plasma samples, and that the rate of this reaction is influenced by blood bag plastic surfac

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1979.19279160282.x

出版商:Blackwell Science Ltd    

年代:1979

数据来源: WILEY

摘要:

Patients with myeloproliferative thrombocythemia have usually been managed with chemotherapy. Generally, removal of platelets from such patients has been performed intermittently to lower the platelet mass rapidly when neurologic or hemorrhagic complications were manifest or concurrently with the initiation of chemotherapy. A platelet count of 1,000,000/μl or less was maintained by the use of repeated plateletpheresis over a period of three months in a patient with essential thrombocythemia, who had frequent syncopal episodes. A second patient with secondary thrombocytosis, who sustained two cerebrovascular accidents, underwent intermittent plateletpheresis over a 22‐day period until her underlying disorder improved. It is concluded from this experience, combined with that of others, that plateletpheresis is not only a life‐saving procedure in an acute episode but may also be used to maintain a lowered platelet count over a prolonged period in selected patients with primary thrombocythemia, in whom chemotherapy cannot be undertaken, and in symptomatic patients with secondary thrombocyth

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1979.19279160283.x

出版商:Blackwell Science Ltd    

年代:1979

数据来源: WILEY

摘要:

With the increased complexity of blood component therapy, it is important to be able to modify physician behavior with reliable educational programs. A standardized educational program on the use of red blood cells and whole blood was tested in 22 hospitals in a regional blood program using medical audit and computer monitoring to evaluate effectiveness. Most hospitals were eager to take advantage of the education program but were unwilling to use the audit‐education cycle. At the same time, computer monitoring of individual hospital ordering and transfusion practices demonstrated an increased utilization of red blood cells in 64 per cent of hospitals with an overall improvement of 8 per cent. The improved use of red blood cells was appreciated within the month of the program and then sustained for six to twelve months at the new leve

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1979.19279160284.x

出版商:Blackwell Science Ltd    

年代:1979

数据来源: WILEY

摘要:

Storage of human erythrocytes in CPO produced a lesion of the erythrocyte membrane manifested by abnormal endocytosis in resealed ghosts and in intact erythrocytes. Endocytosis produced by resealing Ca, Mg, and ATP into ghosts was impaired by five weeks of storage and this defect was promptly reversed by the prior regeneration of ATP in the stored erythrocytes. Drug‐induced endocytosis was studied in intact stored erythrocytes. Vinblastine endocytosis was not affected by storage. Chlorpromazine endocytosis was variably but never completely inhibited by storage, and restoration of ATP occasionally resulted in complete restoration of chlorpromazine endocytosis to base line values. However, primaquine endocytosis was usually totally inhibited after three to four weeks of storage at a time when residual ATP levels were 30 to 50 per cent of base line values. Restoration of ATP levels to at least base line values did not completely restore primaquine endocytosis to control values. Study of primaquine endocytosis provides an opportunity for defining an erythrocyte membrane storage lesio

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1979.19279160285.x

出版商:Blackwell Science Ltd    

年代:1979

数据来源: WILEY

摘要:

A Japanese family with two siblings of phenotype i is presented. Both had a past history of surgical treatment for congenita] cataract. In Japan, 18 individuals of phenotype i, including our case, have been found in ten unrelated families. Seventeen of them had congenital cataract. Cataract was not found in any of the 45 phenotype I members in these families. It is briefly discussed why these two linked and quite rare genes were found in combination only in Japanese persons.

ISSN:0041-1132    

DOI:10.1046/j.1537-2995.1979.19279160286.x

出版商:Blackwell Science Ltd    

年代:1979

数据来源: WILEY